स्वच्छ भारत भविष्य के लिए परिदृश्य

स्वच्छ भारत भविष्य के लिए परिदृश्य अिखल भारतीय राजभाषा वैज्ञानिक संगोष्ठ

ऊष्णीत गृह के बारे में जानें (Know Your Warming Planet-ClimEd Series:1B)

This instructional material "Know Your Warming Planet" has been developed as a part of the Belmont funded project titled "Global Understanding and Learning for Local solutions: Reducing Vulnerability of marine dependent coastal communities" as a means to create awareness and impart climate change knowledge across the target populace

जलवायु परिवर्तन से निपटने के लिए परिवार - ClimEd Series - (Households in combating climate change- ClimEd Series:5B)

Earth’s climate is always changing. In the past, it has gone through warmer and cooler periods which last for thousands of years. The changing climate can be due to natural and anthropogenic activities. Natural causes include changes in earth's orbit, sunspot activity; ocean changes and volcanic eruptions. Recently earth’s climate has been warming alarmingly which is mainly due to human activities like burning of coal, oil and natural gas which can lead to severe impacts across the globe

अधिगम एंव जलवायु परिवर्तन से सामना (Learning & Coping Climate Change-ClimEd Series:2B)

The GULLS project funded by the Belmont Forum addresses coastal vulnerability issues,- specifically the challenges that arise in food security and sustaining coastal livelihoods as a result of global warming and increasing human coastal populations. It seeks to identify adaptation options and strategies for enhancing coastal resilience at the local level and in doing so will contribute to capacity building and local empowerment. The goal of the project is to provide insight to coastal communities to adapt to climate change through trans-disciplinary approach

Iron-dependent mutualism between Chlorella sorokiniana and Ralstonia pickettii forms the basis for a sustainable bioremediation system

Phototrophic communities of autotrophic microalgae and heterotrophic bacteria perform complex tasks of nutrient acquisition and tackling environmental stress but remain underexplored as a basis for the bioremediation of emerging pollutants. In industrial monoculture designs, poor iron uptake by microalgae limits their productivity and biotechnological efficacy. Iron supplementation is expensive and ineffective because iron remains insoluble in an aqueous medium and is biologically unavailable. However, microalgae develop complex interkingdom associations with siderophore-producing bacteria that help solubilize iron and increase its bioavailability. Using dye degradation as a model, we combined environmental isolations and synthetic ecology as a workflow to design a simplified microbial community based on iron and carbon exchange. We established a mutualism between the previously non-associated alga Chlorella sorokiniana and siderophore-producing bacterium Ralstonia pickettii. Siderophore-mediated increase in iron bioavailability alleviated Fe stress for algae and increased the reductive iron uptake mechanism and bioremediation potential. In exchange, C. sorokiniana produced galactose, glucose, and mannose as major extracellular monosaccharides, supporting bacterial growth. We propose that extracellular iron reduction by ferrireductase is crucial for azoreductase-mediated dye degradation in microalgae. These results demonstrate that iron bioavailability, often overlooked in cultivation, governs microalgal growth, enzymatic processes, and bioremediation potential. Our results suggest that phototrophic communities with an active association for iron and carbon exchange have the potential to overcome challenges associated with micronutrient availability, while scaling up bioremediation designs

Protein signatures linking history of miscarriages and metabolic syndrome: a proteomic study among North Indian women

Background Metabolic syndrome (MeS), a constellation of metabolic adversities, and history of miscarriage make women at a higher risk for cardiovascular diseases (CVDs). However, molecular evidence indicating a link between the two phenotypes (history of miscarriage and MeS) among women would offer an opportunity to predict the risk factor for CVDs at an early stage. Thus, the present retrospective study attempts to identify the proteins signatures (if any) to understand the connection between the history of miscarriage and MeS. Methods Age-matched 80 pre-menopausal women who were not on any medical intervention or drugs were recruited from a Mendelian population of the same gene pool. Recruited women were classified into four groups—(a) Group A—absolute cases with history of miscarriage and MeS, (b) Group B—absolute controls without any history of miscarriage and MeS, (c) Group C—cases with MeS but lack any history of miscarriage, (d) Group D—cases with history of miscarriage but lack MeS. Differentially expressed proteins in plasma samples of women from four groups were identified using 2-D gel electrophoresis and mass spectrometry. Results Three case groups (A, C, and D) showed 18 differentially expressed proteins. Nearly 60% of proteins (11/18) were commonly dysregulated in Group C (only with MeS) and Group D (only with miscarriage history). Nearly 40% of proteins (7/18) were commonly dysregulated in the three case groups (Groups A, C, and D), indicating a shared pathophysiology. Four proteins were exclusive but shared by case groups C and D indicating the independent routes for CVDs through MeS or miscarriages. In absolute cases, transthyretin (TTR) showed exclusive upregulation, which was further validated by Western blotting and ELISA. Networking analyses showed the strong association of TTR with haptoglobin, transferrin and ApoA1 hinting toward a cross-talk among these proteins which could be a cause or an effect of TTR upregulation. Conclusion The study provides evidence for molecular link between the history of miscarriage and MeS through a putative role of TTR. However, longitudinal follow-up studies with larger sample size would further help to demonstrate the significance of TTR and other targeted proteins in risk stratification and the onset of CVDs

‘It Takes Two Hands to Clap’: How Gaddi Shepherds in the Indian Himalayas Negotiate Access to Grazing

This article examines the effects of state intervention on the workings of informal institutions that coordinate the communal use and management of natural resources. Specifically it focuses on the case of the nomadic Gaddi shepherds and official attempts to regulate their access to grazing pastures in the Indian Himalayas. It is often predicted that the increased presence of the modern state critically undermines locally appropriate and community-based resource management arrangements. Drawing on the work of Pauline Peters and Francis Cleaver, I identify key instances of socially embedded ‘common’ management institutions and explain the evolution of these arrangements through dynamic interactions between individuals, communities and the agents of the state. Through describing the ‘living space’ of Gaddi shepherds across the annual cycle of nomadic migration with their flocks I explore the ways in which they have been able to creatively reinterpret external interventions, and suggest how contemporary arrangements for accessing pasture at different moments of the annual cycle involve complex combinations of the formal and the informal, the ‘traditional’ and the ‘modern’

Diversity in a promiscuous group of rhizobia from three Sesbania spp. colonizing ecologically distinct habitats of the sem-arid Delhi region

Sesbania-rhizobia associations have immense significance in soil amelioration programs for diverse habitats. Diversity in symbiotic properties, LPS profiles, Sym plasmid and rhizobiophage sensitivity of 28 root-and stem-nodulating bacterial isolates of three Sesbania species ( S. sesban, S. aegyptica and S. rostrata) inhabiting six ecologically distinct sites of semi-arid Delhi region was analyzed. The isolates were highly promiscuous among the symbiotic partners ( Sesbania spp.). The root nodules formed by all the isolates were morphologically similar but they differed in their symbiotic efficiency and effectiveness. 16S rDNA sequence analyses revealed that root nodule isolates of sesbanias belong to diverse rhizobial taxa ( Sinorhizobium saheli, S. meliloti, Rhizobium huautlense) whereas stem-nodule isolates were strictly Azorhizobium caulinodans. Sinorhizobium spp. seem to dominate as microsymbiont partner of Sesbania in the Delhi region. The genetic diversity revealed by cluster analyses based on NPC-PCR reflects sorting of isolates across the ecological gradient. Parallel diversity was also observed in the grouping based on LPS profiles and sym plasmid (NPC-PCR). Segregation of different rhizobial taxa into distinct types/clusters based on LPS and NPC-PCR analyses suggest its significance in the circumscription of the taxa. However, subtypes and subclusters showed their sorting across the ecological gradients. Sesbania rhizobia showed extremely high specificity to rhizobiophages. Enormous diversity in LPS profiles and high specificity of rhizobiophages might be the result of environmental selection pressures operating in ecologically distinct habitats. The ability of sesbanias to enter into effective symbioses with different rhizobial taxa and colonize diverse habitats with various biotic and abiotic stresses appears to contribute to its wide ecological amplitude

Variations in Outer-membrane characteristics of two stem-nodulating bacteria of Sesbania rostrata and its role in tolerance towards diverse stress

Outer-membrane characteristics may determine the survivability of rhizobia under diverse abiotic and biotic stresses. Therefore, the role of lipopolysaccharides (LPS) and membrane proteins of two stem-nodulating bacteria of Sesbania rostrata (Azorhizobium caulinodans ORS571 and Rhizobium sp. WE7) in determining tolerance towards abiotic and biotic stresses (hydrophobics and phages) was investigated. Outer-membrane characteristics (LPS and membrane–protein profiles) of ORS571, WE7 and thirteen standard strains were distinct. ORS571 and WE7 also showed susceptibility towards morphologically distinct phages, i.e., ACSR16 (short-tailed) and WESR29 (long-tailed), respectively. ORS571 and WE7 were tolerant to hydrophobic compounds (triton X-100, rifampicin, crystal violet and deoxycholate). To ascertain the role of outer membrane characteristics in stress tolerance, phage-resistant transconjugant mutants of ORS571 (ORS571-M8 and ORS571-M20) and WE7 (WE7-M9) were developed. LPS- and membrane–protein profiles of mutants differed from that of respective wild types (ORS571 and WE7). In in vitro assay, phages got adsorbed onto purified LPS-membrane protein fractions of wild types. Phages did not adsorb onto membrane fraction of mutants and standard strains. Mutant with reduced expression of LPS (ORS571-M20 and WE7-M9) showed reduced tolerance towards hydrophobics. However, the tolerance was unaffected in mutant (ORS571-M8) where expression of LPS was not reduced but pattern was different. The tolerance level of mutants towards hydrophobics varied with the expression of LPS, whereas the specificity towards phages is correlated with the specific LPS pattern