Detection of clinically relevant yeast in blood: evaluation of methods for RNA extraction and amplification

Objectives: To evaluate different methods of extraction andpurification of RNA from yeast in blood samples to be used inmolecular-based diagnosis. Methods: Different combinations ofcommercially available kits were tested with blood samplesartificially inoculated with yeast cultures. Results: Qiagen kitsperformed better when compared to other commercial kits bothfor RNA extraction and one-step RT-PCR. Conclusions: Kits using ahot-start approach are better; the choice of kits has an importantimpact on the final result of a test

The use of a novel amplification tool for molecular diagnosis of challenging samples

Objective: To obtain adequate amounts of starting material to beused in molecular diagnosis. Methods: Isothermal ramificationamplification. Results: Water samples where Legionella spp wasdiluted and small amount of human DNA from HIV infected patientswere submitted to a new amplification technology (GenomiPhi®kit). Those samples that were previously negative upon PCRbecame positive after the treatment. Conclusion: The newtechnology allows for molecular assays to be performed in samplesof reduced volumes or low concentration

Mycobacterium haemophilum: Emerging or Underdiagnosed in Brazil?

Fleury Ctr Diagnost Med, Microbiol Sect, BR-04344070 Sao Paulo, BrazilUniv Fed Sao Paulo, Sao Paulo, BrazilOswaldo Cruz Hosp, Sao Paulo, BrazilAlbert Einstein Hosp, Sao Paulo, BrazilSirio Libanes Hosp, Sao Paulo, BrazilServidores Estado Hosp, Rio De Janeiro, BrazilAlianca Hosp, Salvador, BA, BrazilLamina Lab, Rio De Janeiro, BrazilInst Oswaldo Cruz, BR-20001 Rio De Janeiro, BrazilUniv Fed Sao Paulo, Sao Paulo, BrazilWeb of Scienc