KYOTO: A System for Mining, Structuring, and Distributing Knowledge Across Languages and Cultures

We outline work performed within the framework of a current EC project. The goal is to construct a language-independent information system for a specific domain (environment/ecology/biodiversity) anchored in a language-independent ontology that is linked to wordnets in seven languages. For each language, information extraction and identification of lexicalized concepts with ontological entries is carried out by text miners (?Kybots?). The mapping of language-specific lexemes to the ontology allows for crosslinguistic identification and translation of equivalent terms. The infrastructure developed within this project enables long-range knowledge sharing and transfer across many languages and cultures, addressing the need for global and uniform transition of knowledge beyond the specific domains addressed here

Renal function after unilateral nephrectomy for Wilms' tumour:The influence of radiation therapy

The effect of therapy on renal function after unilateral nephrectomy for Wilms' tumour was studied. In the second year following unilateral nephrectomy, glomerular filtration rate (GFR) and effective renal plasma flow (ERPF) were estimated simultaneously by measuring I-125-iothalamate clearance and I-131-hippurate clearance. Of 41 evaluable patients, 29 received chemotherapy as sole treatment modality following nephrectomy (group 1); 12 patients additionally received radiation therapy to a field that included the remaining kidney (group 2). Results were expressed as standard deviation scores (z-scores). In group 1, mean z-score for GFR was -0.27 (94.6% of normal) and in group 2 mean z-score was -1.51 (72.7% of normal for two kidneys) (P = 0.022, Mann-Whitney U-test). Mean z-score for ERPF was -0.09 (97.0%) in group 1 and -1.53 (73.8%) in group 2 (P = 0.039). It was concluded that the combination of chemotherapy and radiation therapy, in contrast to chemotherapy alone, negatively affects the ability of the remaining kidney to adjust its function after the loss of its counterpart

Higher high density lipoprotein cholesterol associated with moderate alcohol consumption is not related to altered plasma lecithin: cholesterol acyltransferase and lipid transfer protein activity levels

Lecithin:cholesterol acyltransferase (LCAT), cholesteryl ester transfer protein (CETP) and phospholipid transfer protein (PLTP) are important factors involved in HDL metabolism. Altered plasma activity levels of these factors could play a role in the increase in high density lipoprotein (HDL) cholesterol associated with moderate alcohol consumption. We measured plasma LCAT, CETP and PLTP activities with exogenous substrate assays, as well as lipoproteins and HDL lipids in 6 alcohol-abstaining men, 18 matched men who used less than or equal to 1 and 18 men who used >1 alcohol-containing drinks per day. Plasma cholesterol and triglycerides were similar in the three groups. HDL total cholesterol, HDL cholesteryl ester, HDL free cholesterol and HDL triglycerides were higher in the alcohol drinkers compared to the abstainers (all P <0.05). No differences in plasma LCAT, CETP and PLTP activity levels were observed between the three groups. Analysis of covariance also demonstrated that the use of alcohol was associated with higher HDL cholesterol (P <0.04), whereas plasma LCAT, CETP and PLTP activity levels were not related to alcohol consumption. Furthermore, HDL cholesteryl ester was positively associated with; LCAT activity (P <0.001), PLTP activity (P <0.01) and alcohol intake (P <0.04) and negatively with plasma triglycerides (P <0.001) and CETP activity (P <0.03), indicating that alcohol influenced HDL cholesteryl ester independently from these biochemical parameters. The higher HDL cholesterol associated with moderate alcohol consumption is, therefore, unlikely to be caused by an effect on plasma LCAT, CETP or PLTP activity levels. Copyright (C) 1997 Elsevier Science B.V

Supplementary Material for: C-terminal PEGylation improves SAAP-148 peptide's immunomodulatory activities

Synthetic antibacterial and anti-biofilm peptide (SAAP)-148 was developed to combat bacterial infections not effectively treatable with current antibiotics. SAAP-148 is highly effective against antimicrobial-resistant (AMR) bacteria without inducing resistance, however challenges for further development of SAAP-148 include its cytotoxicity and short circulation half-life. To circumvent these drawbacks a library of SAAP-148 linked to polyethylene glycol (PEG) groups of various lengths was screened for in vitro antibacterial activity and hemolytic activity. Results indicated that PEGylated SAAP-148 variants combine antibacterial activities with reduced hemolysis compared to SAAP-148. Interestingly, pro-inflammatory immunomodulatory activities of SAAP-148 were enhanced upon C-terminal PEGylation, with SAAP-148-PEG27 showing most effect. SAAP-148-PEG27 enhanced SAAP-148’s capacity to chemoattract human neutrophils and was able to more efficiently (re)direct M-CSF-induced monocyte-macrophage differentiation towards type 1 macrophages compared to SAAP-148. Furthermore, dendritic cells with a stronger mature expression profile were produced if monocytes were exposed to SAAP-148-PEG27 during monocyte-immature dendritic cell differentiation in comparison to SAAP-148. Parameters that influenced the immunomodulatory activities of the peptide-PEG conjugate include i) the length of the PEG-group, ii) the position of PEG conjugation, and iii) the peptide sequence. Together, these results indicate that SAAP-148-PEG27 is highly effective in redirecting monocyte-macrophage differentiation towards a proinflammatory phenotype and promoting monocyte-mature dendritic cells development. Therefore, SAAP-148-PEG27 may be a promising agent to modulate inadequate immune responses in case of tumors and chronically infected wounds

KYOTO: A system for mining, structuring, and distributing knowledge across languages and cultures

We outline work to be carried out within the framework of an impending EC project. The goal is to construct a language-independent information system for a specific domain (environment/ecology) anchored in a language-independent ontology that is linked to WordNets in several languages. For each language, information extraction and identification of lexicalized concepts with ontological entries will be done by text miners ("Kybots"). The mapping of language-specific lexemes to the ontology allows for crosslinguistic identification and translation of equivalent terms. The infrastructure developed within this project will enable long-range knowledge sharing and transfer to many languages and cultures, addressing the need for global and uniform transition of knowledge beyond the domain of ecology and environment addressed here