ESA/ELGRA Gravity-Related Research Summer School: an introduction to microgravity and hypergravity research for university students

The European Low Gravity Research Association (ELGRA) and the European Space Agency (ESA) co-organise since 2016 a Summer School on gravity-related research in the frame of ESA Academy’s Training and Learning Programme. This Summer School is organised every year, in June, at the ESA Education Training Centre located in ESA’s European Space Security and Education Centre (ESEC), Belgium. The Summer School explains the fundamentals of performing research at different gravity levels and offers an overview of current research activity under microgravity and hypergravity conditions in life and physical sciences. Over four and a half intensive days, up to 30 Bachelor and Master students from ESA Member States, Canada and Slovenia, attend stimulating lectures, and work within small groups to devise project ideas for prospective experiments. Gravity-related research is introduced to these future scientists and engineers by experienced professionals from across the European space and research sector. These trainers are ELGRA members and ESA experts, freely sharing their experience and know-how with the students, including their day-to-day work and research experience in biology, human physiology, physics and engineering. Each year the programme incorporates new elements to enhance the experience for the students based on their feedback. 104 university students and 43 different experts have already participated in this Summer School.The Summer School is a jointly funded initiative from the European Space Agency (ESA) and the European Low Gravity Research Association (ELGRA).Peer ReviewedPostprint (author's final draft

ESA Academy activities during COVID-19

The ESA Academy is the ESA Education Office’s overarching programme for university students. The Academy’s portfolio consists of both ‘hands-on’ activities, and a Training and Learning Programme. Conventionally both of these elements involve a significant number of in person events, for example training sessions, workshops and test and launch campaigns. The educational nature and practical aspects of such events has traditionally necessitated in person participation. Additionally, most of the Academy’s ‘hands-on’ programmes revolve around student teams designing, building, testing and operating an experiment or spacecraft, activities which rely on the availability and delivery of commercial components, and access to manufacturing, testing and launch facilities, and laboratories. In March 2020, as the COVID-19 pandemic, and associated restrictions, began to take hold in Europe, nearly all the ESA Academy programmes were affected. Despite the challenges, the Academy continued to deliver activities, and the student teams participating in the Academy’s programmes continued to achieve major milestones, including launching experiments to the ISS, CubeSat testing and launch and execution of micro- and hyper-gravity experiments. This paper explores the challenges faced during COVID-19 and how both the programmes and the students participating in the programmes adapted to meet their educational, scientific, and technical goals. Furthermore, the longer-term adaptation of some of these changes into the future execution of the programmes is discusse

The Combination of Particle Irradiation With the Hedgehog Inhibitor GANT61 Differently Modulates the Radiosensitivity and Migration of Cancer Cells Compared to X-Ray Irradiation

Due to the advantages of charged particles compared to conventional radiotherapy, a vast increase is noted in the use of particle therapy in the clinic. These advantages include an improved dose deposition and increased biological effectiveness. Metastasis is still an important cause of mortality in cancer patients and evidence has shown that conventional radiotherapy can increase the formation of metastasizing cells. An important pathway involved in the process of metastasis is the Hedgehog (Hh) signaling pathway. Recent studies have demonstrated that activation of the Hh pathway, in response to X-rays, can lead to radioresistance and increased migratory, and invasive capabilities of cancer cells. Here, we investigated the effect of X-rays, protons, and carbon ions on cell survival, migration, and Hh pathway gene expression in prostate cancer (PC3) and medulloblastoma (DAOY) cell lines. In addition, the potential modulation of cell survival and migration by the Hh pathway inhibitor GANT61 was investigated. We found that in both cell lines, carbon ions were more effective in decreasing cell survival and migration as well as inducing more significant alterations in the Hh pathway genes compared to X-rays or protons. In addition, we show here for the first time that the Hh inhibitor GANT61 is able to sensitize DAOY medulloblastoma cells to particle radiation (proton and carbon ion) but not to conventional X-rays. This important finding demonstrates that the results of combination treatment strategies with X-ray radiotherapy cannot be automatically extrapolated to particle therapy and should be investigated separately. In conclusion, combining GANT61 with particle radiation could offer a benefit for specific cancer types with regard to cancer cell survival

Gravity-related immunological changes in human whole blood cultured under simulated microgravity using an in vitro cytokine release assay

Although immune dysfunction by space conditions has been reported postflight, as well as during ground-based experiments, the cause(s) and nature of the immunological changes are not completely understood. Microgravity has been suggested as one of the factors responsible for the observed immune dysregulation. The goal of this study was to assess immune changes in simulated microgravity (s-G) using an in vitro cytokine release assay. The effect of s-G provided by the desktop random positioning machine on cell-mediated immunity was examined by analyzing interleukin 2 (IL-2), interferon- (IFN-), tumor necrosis factor alpha (TNF-), and interleukin 10 (IL-10), in response to immune cell stimulation in whole blood samples (n=10). Stimuli used were bacterial recall antigens, pokeweed mitogen (PWM), lipopolysaccharide (LPS), or heat-killed Listeria monocytogenes (HKLM). S-G caused an overall inhibition of the IL-2 and IFN- responses to recall antigen and mitogen stimulation. More specifically, s-G most strongly influenced the levels of all four cytokines elicited by bacterial recall antigen stimulation. In contrast, HKLM-induced TNF- secretion was elevated. The average concentrations of TNF- in response to PWM and LPS and IL-10 release stimulated by PWM, LPS, and HKLM were not significantly altered by s-G. However, a variable response between individual subjects could be observed. In conclusion, our results demonstrate that the in vitro cytokine release assay can detect gravity-related immune alterations. Furthermore, the use of multiple stimuli and the associated changes in cytokine secretion has the potential to reveal information on the underlying mechanisms affected by s-G

The Combination of Particle Irradiation With the Hedgehog Inhibitor GANT61 Differently Modulates the Radiosensitivity and Migration of Cancer Cells Compared to X-Ray Irradiation

Due to the advantages of charged particles compared to conventional radiotherapy, a vast increase is noted in the use of particle therapy in the clinic. These advantages include an improved dose deposition and increased biological effectiveness. Metastasis is still an important cause of mortality in cancer patients and evidence has shown that conventional radiotherapy can increase the formation of metastasizing cells. An important pathway involved in the process of metastasis is the Hedgehog (Hh) signaling pathway. Recent studies have demonstrated that activation of the Hh pathway, in response to X-rays, can lead to radioresistance and increased migratory, and invasive capabilities of cancer cells. Here, we investigated the effect of X-rays, protons, and carbon ions on cell survival, migration, and Hh pathway gene expression in prostate cancer (PC3) and medulloblastoma (DAOY) cell lines. In addition, the potential modulation of cell survival and migration by the Hh pathway inhibitor GANT61 was investigated. We found that in both cell lines, carbon ions were more effective in decreasing cell survival and migration as well as inducing more significant alterations in the Hh pathway genes compared to X-rays or protons. In addition, we show here for the first time that the Hh inhibitor GANT61 is able to sensitize DAOY medulloblastoma cells to particle radiation (proton and carbon ion) but not to conventional X-rays. This important finding demonstrates that the results of combination treatment strategies with X-ray radiotherapy cannot be automatically extrapolated to particle therapy and should be investigated separately. In conclusion, combining GANT61 with particle radiation could offer a benefit for specific cancer types with regard to cancer cell survival.status: publishe