Experimental study on the development of a micro-drilling cycle using ultrashort laser pulses

Microholes for the production of high precision devices were obtained by ultrashort pulsed laser machining of martensitic stainless steels. A micro-drilling cycle based on the sequence of a drilling through phase, an enlargement and finishing phase is proposed in order to solve the trade-off between process time and quality of the ablated surfaces without making use of complex design of experiments. The three phases were studied taking into account the evolution of the microhole shape as a function of the main process parameters (number of passes per phase, incidence angle and radius of the beam trajectory respect to the hole's axis). Experiments testified that the drilling strategy was able to produce cylindrical holes with diameter of 180±2 μm on a 350 μm thick plate in total absence of burrs and debris within a drilling time of 3.75 s. Repeatability tests showed a process capability of nearly 99%. SEM inspection of the inner surface of the microholes showed the presence of elongated and periodic ripples whose size and inclination can be controlled adjusting the incidence angle of the beam over the tapered surface before the ultimate finishing phase

Mapping metals at high redshift with far-infrared lines

Cosmic metal enrichment is one of the key physical processes regulating galaxy formation and the evolution of the intergalactic medium (IGM). However, determining the metal content of the most distant galaxies has proven so far almost impossible; also, absorption line experiments at $z\sim6$ become increasingly difficult because of instrumental limitations and the paucity of background quasars. With the advent of ALMA, far-infrared emission lines provide a novel tool to study early metal enrichment. Among these, the [CII] line at 157.74 $\mu$m is the most luminous line emitted by the interstellar medium of galaxies. It can also resonant scatter CMB photons inducing characteristic intensity fluctuations ($\Delta I/I_{CMB}$) near the peak of the CMB spectrum, thus allowing to probe the low-density IGM. We compute both [CII] galaxy emission and metal-induced CMB fluctuations at $z\sim 6$ by using Adaptive Mesh Refinement cosmological hydrodynamical simulations and produce mock observations to be directly compared with ALMA BAND6 data ($\nu_{obs}\sim 272$ GHz). The [CII] line flux is correlated with $M_{UV}$ as $\log(F_{peak}/\mu{\rm Jy})=-27.205-2.253\,M_{UV}-0.038\,M_{UV}^2$. Such relation is in very good agreement with recent ALMA observations (e.g. Maiolino et al. 2015; Capak et al. 2015) of $M_{UV}<-20$ galaxies. We predict that a $M_{UV}=-19$ ($M_{UV}=-18$) galaxy can be detected at $4\sigma$ in $\simeq40$ (2000) hours, respectively. CMB resonant scattering can produce $\simeq\pm 0.1\,\mu$Jy/beam emission/absorptions features that are very challenging to be detected with current facilities. The best strategy to detect these signals consists in the stacking of deep ALMA observations pointing fields with known $M_{UV}\simeq-19$ galaxies. This would allow to simultaneously detect both [CII] emission from galactic reionization sources and CMB fluctuations produced by $z\sim6$ metals.Comment: 13 pages, 6 figure

Ochrobactrum sp. MPV1 from a dump of roasted pyrites can be exploited as bacterial catalyst for the biogenesis of selenium and tellurium nanoparticles

Background: Bacteria have developed different mechanisms for the transformation of metalloid oxyanions to non-toxic chemical forms. A number of bacterial isolates so far obtained in axenic culture has shown the ability to bioreduce selenite and tellurite to the elemental state in different conditions along with the formation of nanoparticles-both inside and outside the cells-characterized by a variety of morphological features. This reductive process can be considered of major importance for two reasons: firstly, toxic and soluble (i.e. bioavailable) compounds such as selenite and tellurite are converted to a less toxic chemical forms (i.e. zero valent state); secondly, chalcogen nanoparticles have attracted great interest due to their photoelectric and semiconducting properties. In addition, their exploitation as antimicrobial agents is currently becoming an area of intensive research in medical sciences. Results: In the present study, the bacterial strain Ochrobactrum sp. MPV1, isolated from a dump of roasted arsenopyrites as residues of a formerly sulfuric acid production near Scarlino (Tuscany, Italy) was analyzed for its capability of efficaciously bioreducing the chalcogen oxyanions selenite (SeO32-) and tellurite (TeO32-) to their respective elemental forms (Se0 and Te0) in aerobic conditions, with generation of Se- and Te-nanoparticles (Se- and TeNPs). The isolate could bioconvert 2 mM SeO32- and 0.5 mM TeO32- to the corresponding Se0 and Te0 in 48 and 120 h, respectively. The intracellular accumulation of nanomaterials was demonstrated through electron microscopy. Moreover, several analyses were performed to shed light on the mechanisms involved in SeO32- and TeO32- bioreduction to their elemental states. Results obtained suggested that these oxyanions are bioconverted through two different mechanisms in Ochrobactrum sp. MPV1. Glutathione (GSH) seemed to play a key role in SeO32- bioreduction, while TeO32- bioconversion could be ascribed to the catalytic activity of intracellular NADH-dependent oxidoreductases. The organic coating surrounding biogenic Se- and TeNPs was also characterized through Fourier-transform infrared spectroscopy. This analysis revealed interesting differences among the NPs produced by Ochrobactrum sp. MPV1 and suggested a possible different role of phospholipids and proteins in both biosynthesis and stabilization of such chalcogen-NPs. Conclusions: In conclusion, Ochrobactrum sp. MPV1 has demonstrated to be an ideal candidate for the bioconversion of toxic oxyanions such as selenite and tellurite to their respective elemental forms, producing intracellular Se- and TeNPs possibly exploitable in biomedical and industrial applications.[Figure not available: see fulltext.

Antimicrobial activity of biogenically produced spherical Se-nanomaterials embedded in organic material against Pseudomonas aeruginosa and Staphylococcus aureus strains on hydroxyapatite-coated surfaces

In an effort to prevent the formation of pathogenic biofilms on hydroxyapatite (HA)-based clinical devices and surfaces, we present a study evaluating the antimicrobial efficacy of Spherical biogenic Se-Nanostructures Embedded in Organic material (Bio Se-NEMO-S) produced by Bacillus mycoides SelTE01 in comparison with two different chemical selenium nanoparticle (SeNP) classes. These nanomaterials have been studied as potential antimicrobials for eradication of established HA-grown biofilms, for preventing biofilm formation on HA-coated surfaces and for inhibition of planktonic cell growth of Pseudomonas aeruginosa NCTC 12934 and Staphylococcus aureus ATCC 25923. Bio Se-NEMO resulted more efficacious than those chemically produced in all tested scenarios. Bio Se-NEMO produced by B.&nbsp;mycoides SelTE01 after 6 or 24&nbsp;h of Na 2 SeO 3 exposure show the same effective antibiofilm activity towards both P.&nbsp;aeruginosa and S.&nbsp;aureus strains at 0.078&nbsp;mg&nbsp;ml −1 (Bio Se-NEMO 6 ) and 0.3125&nbsp;mg&nbsp;ml −1 (Bio Se-NEMO 24 ). Meanwhile, chemically synthesized SeNPs at the highest tested concentration (2.5&nbsp;mg&nbsp;ml −1 ) have moderate antimicrobial activity. The confocal laser scanning micrographs demonstrate that the majority of the P.&nbsp;aeruginosa and S.&nbsp;aureus cells exposed to biogenic SeNPs within the biofilm are killed or eradicated. Bio Se-NEMO therefore displayed good antimicrobial activity towards HA-grown biofilms and planktonic cells, becoming possible candidates as new antimicrobials

INEFICÁCIA DAS PENAS NOS CRIMES DE TRÂNSITO

O presente trabalho tem como objetivo apresentar uma análise da ineficácia das penas nos crimes de trânsito. Para sua realização foram feitas pesquisas documentais, em livros, monografias, artigos acadêmicos e sites. Demonstra-se a necessidade de um novo entendimento sobre a educação de Trânsito, difundindo a ideia social sobre qual é o objetivo da pena em relação aos crimes de Trânsito, e a maior eficácia das penas alternativas para estes delitos. Adotando-se o Princípio da Mínima Intervenção com a aplicação de penas alternativas em vez de penas de restrição de liberdade nos conflitos de trânsito, haveria mínima contribuição desse tipo de crime no aumento da população carcerária e a diminuição da reincidência desses crimes no futuro. O Sistema Penal atual não contribui para ressocialização dos infratores, muito pelo contrário. É preciso um trabalho coerente, uma educação eficaz, mudanças de pensamentos, eficácia nas leis. Só depois disso podemos pensar em criminalizar todos os atos humanos impensados. Educar ao invés de punir ainda é a melhor opção

The assembly of "normal" galaxies at z=7 probed by ALMA

We report new deep ALMA observations aimed at investigating the [CII]158um line and continuum emission in three spectroscopically confirmed Lyman Break Galaxies at 6.8<z<7.1, i.e. well within the re-ionization epoch. With Star Formation Rates of SFR ~ 5-15 Msun/yr these systems are much more representative of the high-z galaxy population than other systems targeted in the past by millimeter observations. For the galaxy with the deepest observation we detect [CII] emission at redshift z=7.107, fully consistent with the Lyalpha redshift, but spatially offset by 0.7" (4 kpc) from the optical emission. At the location of the optical emission, tracing both the Lyalpha line and the far-UV continuum, no [CII] emission is detected in any of the three galaxies, with 3sigma upper limits significantly lower than the [CII] emission observed in lower reshift galaxies. These results suggest that molecular clouds in the central parts of primordial galaxies are rapidly disrupted by stellar feedback. As a result, [CII] emission mostly arises from more external accreting/satellite clumps of neutral gas. These findings are in agreement with recent models of galaxy formation. Thermal far-infrared continuum is not detected in any of the three galaxies. However, the upper limits on the infrared-to-UV emission ratio do not exceed those derived in metal- and dust-poor galaxies.Comment: 15 pages, 9 figures, MNRAS in press, replaced with accepted versio

ALMA suggests outflows in z ~ 5.5 galaxies

We present the first attempt to detect outflows from galaxies approaching the Epoch of Reionization (EoR) using a sample of 9 star-forming ($\rm SFR=31\pm 20~M_{\odot}~yr^{-1}$) $z\sim 5.5$ galaxies for which the [CII]158$\mu$m line has been previously obtained with ALMA. We first fit each line with a Gaussian function and compute the residuals by subtracting the best fitting model from the data. We combine the residuals of all sample galaxies and find that the total signal is characterised by a flux excess of $\sim 0.5$ mJy extended over $\sim 1000$ km~s$^{-1}$. Although we cannot exclude that part of this signal is due to emission from faint satellite galaxies, we show that the most probable explanation for the detected flux excess is the presence of broad wings in the [CII] lines, signatures of starburst-driven outflows. We infer an average outflow rate of $\rm \dot{M}=54\pm23~ M_{\odot}~yr^{-1}$, providing a loading factor $\eta=\rm \dot{M}/SFR=1.7\pm1.3$ in agreement with observed local starbursts. Our interpretation is consistent with outcomes from zoomed hydro-simulations of {\it Dahlia}, a $z\sim 6$ galaxy ($\rm SFR\sim 100~\rm M_{\odot}~yr^{-1}$) whose feedback-regulated star formation results into an outflow rate $\rm \dot{M}\sim 30~ M_{\odot}~yr^{-1}$. The quality of the ALMA data is not sufficient for a detailed analysis of the [CII] line profile in individual galaxies. Nevertheless, our results suggest that starburst-driven outflows are in place in the EoR and provide useful indications for future ALMA campaigns. Deeper observations of the [CII] line in this sample are required to better characterise feedback at high-$z$ and to understand the role of outflows in shaping early galaxy formation

The impact of chemistry on the structure of high-z galaxies

To improve our understanding of high-z galaxies we study the impact of H$_{2}$ chemistry on their evolution, morphology and observed properties. We compare two zoom-in high-resolution (30 pc) simulations of prototypical $M_{\star}\sim 10^{10} {\rm M}_{\odot}$ galaxies at $z=6$. The first, "Dahlia", adopts an equilibrium model for H$_{2}$ formation, while the second, "Alth{\ae}a", features an improved non-equilibrium chemistry network. The star formation rate (SFR) of the two galaxies is similar (within 50\%), and increases with time reaching values close to 100 ${\rm M}_{\odot}/\rm yr$ at $z=6$. They both have SFR-stellar mass relation consistent with observations, and a specific SFR of $\simeq 5\, {\rm Gyr}^{-1}$. The main differences arise in the gas properties. The non-equilibrium chemistry determines the H$\rightarrow$ H$_{2}$~transition to occur at densities $> 300\,{cm}^{-3}$, i.e. about 10 times larger than predicted by the equilibrium model used for Dahlia. As a result, Alth{\ae}a features a more clumpy and fragmented morphology, in turn making SN feedback more effective. Also, because of the lower density and weaker feedback, Dahlia sits $3\sigma$ away from the Schmidt-Kennicutt relation; Alth{\ae}a, instead nicely agrees with observations. The different gas properties result in widely different observables. Alth{\ae}a outshines Dahlia by a factor of 7 (15) in [CII]~$157.74\,\mu{\rm m}$ (H$_{2}$~$17.03\,\mu{\rm m}$) line emission. Yet, Alth{\ae}a is under-luminous with respect to the locally observed [CII]-SFR relation. Whether this relation does not apply at high-z or the line luminosity is reduced by CMB and metallicity effects remains as an open question

The interstellar medium of high-redshift galaxies: Gathering clues from C III] and [C II] lines

Context. A tight relation between [C II] 158 μm line luminosity and the star formation rate (SFR) has been observed for local galaxies. At high redshift (z &gt; 5), galaxies instead deviate downwards from the local Σ[C II] − ΣSFR relation. This deviation might be caused by different interstellar medium (ISM) properties in galaxies at early epochs. Aims. To test this hypothesis, we combined the [C II] and SFR data with C III] 1909 Å line observations and our physical models. We additionally investigated how ISM properties, such as burstiness, κs, total gas density, n, and metallicity, Z, affect the deviation from the Σ[C II] − ΣSFR relation in these sources. Methods. We present the VLT/X-shooter observations targeting the C III] λ1909 line emission in three galaxies at 5.5 &lt; z &lt; 7.0. We include archival X-shooter data of two other sources at 5.5 &lt; z &lt; 7.0 and the VLT/MUSE archival data of six galaxies at z ∼ 2. We extend our sample of galaxies with eleven star-forming systems at 6 &lt; z &lt; 7.5, with either C III] or [C II] detection reported in the literature. Results. We detected C III] λλ1907, 1909 line emission in HZ10 and we derived the intrinsic, integrated flux of the C III] λ1909 line. We constrained the ISM properties for our sample of galaxies, κs, n, and Z, by applying our physically motivated model based on the MCMC algorithm. For the most part, high-z star-forming galaxies show subsolar metallicities. The majority of the sources have log(κs) ≳ 1, that is, they overshoot the Kennicutt–Schmidt (KS) relation by about one order of magnitude. Conclusions. Our findings suggest that the whole KS relation might be shifted upwards at early times. Furthermore, all the high-z galaxies of our sample lie below the Σ[C II] − ΣSFR local relation. The total gas density, n, shows the strongest correlation with the deviation from the local Σ[C II] − ΣSFR relation, namely, low-density high-z systems have lower [C II] surface brightness, in agreement with theoretical models

Untargeted Metabolomics Investigation on Selenite Reduction to Elemental Selenium by Bacillus mycoides SeITE01

Bacillus mycoides SeITE01 is an environmental isolate that transforms the oxyanion selenite ((Formula presented.)) into the less bioavailable elemental selenium (Se0) forming biogenic selenium nanoparticles (Bio-SeNPs). In the present study, the reduction of sodium selenite (Na2SeO3) by SeITE01 strain and the effect of (Formula presented.) exposure on the bacterial cells was examined through untargeted metabolomics. A time-course approach was used to monitor both cell pellet and cell free spent medium (referred as intracellular and extracellular, respectively) metabolites in SeITE01 cells treated or not with (Formula presented.). The results show substantial biochemical changes in SeITE01 cells when exposed to (Formula presented.). The initial uptake of (Formula presented.) by SeITE01 cells (3h after inoculation) shows both an increase in intracellular levels of 4-hydroxybenzoate and indole-3-acetic acid, and an extracellular accumulation of guanosine, which are metabolites involved in general stress response adapting strategies. Proactive and defensive mechanisms against (Formula presented.) are observed between the end of lag (12h) and beginning of exponential (18h) phases. Glutathione and N-acetyl-L-cysteine are thiol compounds that would be mainly involved in Painter-type reaction for the reduction and detoxification of (Formula presented.) to Se0. In these growth stages, thiol metabolites perform a dual role, both acting against the toxic and harmful presence of the oxyanion and as substrate or reducing sources to scavenge ROS production. Moreover, detection of the amino acids L-threonine and ornithine suggests changes in membrane lipids. Starting from stationary phase (24 and 48h), metabolites related to the formation and release of SeNPs in the extracellular environment begin to be observed. 5-hydroxyindole acetate, D-[+]-glucosamine, 4-methyl-2-oxo pentanoic acid, and ethanolamine phosphate may represent signaling strategies following SeNPs release from the cytoplasmic compartment, with consequent damage to SeITE01 cell membranes. This is also accompanied by intracellular accumulation of trans-4-hydroxyproline and L-proline, which likely represent osmoprotectant activity. The identification of these metabolites suggests the activation of signaling strategies that would protect the bacterial cells from (Formula presented.) toxicity while it is converting into SeNPs