Ecological Impacts of Nitrogen Deposition, Drought and Nonnative Plant Invasion on Coastal Sage Scrub of the Santa Monica Mountains

Multiple drivers of global environmental change increasingly threaten native ecosystems, including atmospheric pollution and resulting changes in climate and nutrient cycling, and the globalization of species. These factors may also have complex and interactive ecological effects. Nitrogen (N) deposition, the input of reactive N from the atmosphere to the earth’s surface, is increasing dramatically worldwide due to anthropogenic air pollution, with the potential to negatively impact terrestrial plant diversity. Elevated N deposition may also interact with other drivers of environmental change, for example by promoting the invasion of nonnative plant species, or increasing plant susceptibility to drought or other secondary stressors. Perhaps nowhere in the U.S. is this of more immediate environmental concern than in southern California, which is a global hotspot of biodiversity and one of the most air-polluted and populous parts of the country. High levels of N deposition have been implicated in the widespread conversion of coastal sage scrub (CSS) to annual grasslands dominated by nonnative grasses and forbs. The Santa Monica Mountains National Recreation Area of southern California protects a substantial area of remaining CSS, but due to the park’s proximity to the City of Los Angeles, stands of CSS nearest urban areas may be subject to high levels of N deposition. The state of California is also in the midst of a record-breaking drought, beginning in 2011, and this may exacerbate the negative impacts of N deposition and nonnative plant species. The objective of this work is to explore the effects of N deposition, drought and nonnative plant invasion on CSS of the Santa Monica Mountains at multiple ecologically relevant scales. I explored relationships of atmospheric N pollution and N deposition with native plant richness and cover of nonnative species at the landscape level, finding N deposition reduces richness of native herbaceous species and is associated with higher nonnative cover. I also investigated the impact of multiple realistic levels of N addition on CSS in a field fertilization experiment on the low end of the N deposition gradient during a period that coincided with the California drought. Through this experiment, I demonstrated increased N availability may reduce water-use efficiency and drought tolerance of native shrubs, resulting in increased dieback, while concomitantly favoring nonnative annual species. Finally, I explored the role of the soil microbial community in mediating impacts of these factors on native and nonnative plant species, finding that N-impacted soil communities may provide less protection against drought in native shrub seedlings and increase growth of invasive plant species. Collectively, these results illustrate the significant ecological threat of increased N deposition on the severely threated CSS of southern California, and potential interactions with other drivers of global change such as extreme drought, and nonnative plant invasion

Ecological Impacts of Nitrogen Deposition, Drought and Nonnative Plant Invasion on Coastal Sage Scrub of the Santa Monica Mountains

Multiple drivers of global environmental change increasingly threaten native ecosystems, including atmospheric pollution and resulting changes in climate and nutrient cycling, and the globalization of species. These factors may also have complex and interactive ecological effects. Nitrogen (N) deposition, the input of reactive N from the atmosphere to the earth’s surface, is increasing dramatically worldwide due to anthropogenic air pollution, with the potential to negatively impact terrestrial plant diversity. Elevated N deposition may also interact with other drivers of environmental change, for example by promoting the invasion of nonnative plant species, or increasing plant susceptibility to drought or other secondary stressors. Perhaps nowhere in the U.S. is this of more immediate environmental concern than in southern California, which is a global hotspot of biodiversity and one of the most air-polluted and populous parts of the country. High levels of N deposition have been implicated in the widespread conversion of coastal sage scrub (CSS) to annual grasslands dominated by nonnative grasses and forbs. The Santa Monica Mountains National Recreation Area of southern California protects a substantial area of remaining CSS, but due to the park’s proximity to the City of Los Angeles, stands of CSS nearest urban areas may be subject to high levels of N deposition. The state of California is also in the midst of a record-breaking drought, beginning in 2011, and this may exacerbate the negative impacts of N deposition and nonnative plant species. The objective of this work is to explore the effects of N deposition, drought and nonnative plant invasion on CSS of the Santa Monica Mountains at multiple ecologically relevant scales. I explored relationships of atmospheric N pollution and N deposition with native plant richness and cover of nonnative species at the landscape level, finding N deposition reduces richness of native herbaceous species and is associated with higher nonnative cover. I also investigated the impact of multiple realistic levels of N addition on CSS in a field fertilization experiment on the low end of the N deposition gradient during a period that coincided with the California drought. Through this experiment, I demonstrated increased N availability may reduce water-use efficiency and drought tolerance of native shrubs, resulting in increased dieback, while concomitantly favoring nonnative annual species. Finally, I explored the role of the soil microbial community in mediating impacts of these factors on native and nonnative plant species, finding that N-impacted soil communities may provide less protection against drought in native shrub seedlings and increase growth of invasive plant species. Collectively, these results illustrate the significant ecological threat of increased N deposition on the severely threated CSS of southern California, and potential interactions with other drivers of global change such as extreme drought, and nonnative plant invasion

Body-composition changes in the Comprehensive Assessment of Long-term Effects of Reducing Intake of Energy (CALERIE)-2 study: A 2-y randomized controlled trial of calorie restriction in nonobese humans

Background: Calorie restriction (CR) retards aging and increases longevity in many animal models. However, it is unclear whether CR can be implemented in humans without adverse effects on body composition.Objective: We evaluated the effect of a 2-y CR regimen on body composition including the influence of sex and body mass index (BMI; in kg/m2) among participants enrolled in CALERIE-2 (Comprehensive Assessment of Long-term Effects of Reducing Intake of Energy), a multicenter, randomized controlled trial.Design: Participants were 218 nonobese (BMI: 21.9-28.0) adults aged 21-51 y who were randomly assigned to 25% CR (CR, n = 143) or ad libitum control (AL, n = 75) in a 2:1 ratio. Measures at baseline and 12 and 24 mo included body weight, waist circumference, fat mass (FM), fat-free mass (FFM), and appendicular mass by dual-energy X-ray absorptiometry; activity-related energy expenditure (AREE) by doubly labeled water; and dietary protein intake by self-report. Values are expressed as means ± SDs.Results: The CR group achieved 11.9% ± 0.7% CR over 2-y and had significant decreases in weight (-7.6 ± 0.3 compared with 0.4 ± 0.5 kg), waist circumference (-6.2 ± 0.4 compared with 0.9 ± 0.5 cm), FM (-5.4 ± 0.3 compared with 0.5 ± 0.4 kg), and FFM (-2.0 ± 0.2 compared with -0.0 ± 0.2 kg) at 24 mo relative to the AL group (all between-group P < 0.001). Moreover, FFM as a percentage of body weight at 24 mo was higher, and percentage of FM was lower in the CR group than in the AL. AREE, but not protein intake, predicted preservation of FFM during CR (P < 0.01). Men in the CR group lost significantly more trunk fat (P = 0.03) and FFM expressed as a percentage of weight loss (P < 0.001) than women in the CR group.Conclusions: Two years of CR had broadly favorable effects on both whole-body and regional adiposity that could facilitate health span in humans. The decrements in FFM were commensurate with the reduced body mass; although men in the CR group lost more FFM than the women did, the percentage of FFM in the men in the CR group was higher than at baseline. CALERIE was registered at clinicaltrials.gov as NCT00427193