Cultural landscape: Towards the design of a nocturnal lightscape

The current debate on the cultural landscape is focused on an inclusive definition. It proposes active enhancement practices and local planning tools that are also dedicated to the study of scenic-perceptive components and visual values. However, the current indications are limited to the definition of day images of sites, and are not based on investigations of the corresponding nocturnal images. The legislation and the recommendations in force in the field of lighting supply performance requirements, related to street and urban lighting, do not provide any indications regarding territorial contexts or widespread heritage sites. This paper presents a critical analysis of the lighting condition of different territorial contexts, places of widespread heritage, in particular with reference to the Italian context. The present situation has been investigated through a qualitative analysis, based on a comparison between day and night images, and a quantitative analysis, developed through a measurement campaign on representative case studies of the recurrent territorial context. In most cases, an absence of a specific lighting design strategy, aimed at defining a suitable nocturnal perception of the cultural landscape, has emerged from the collected data. The aim of the work has been to underline the importance of the study of nocturnal images and to show the possibility of developing an analysis and design methodology for these contexts. This approach should be based on a systemic vision, which should allow not only single monuments to be valorised, but also the places and landscapes that characterise a territory from a morphological and cultural point of view

La provincia de Córdoba en un escenario de generación distribuida bajo un sistema de feed in tariff

En primer lugar se contextualiza el concepto de Generación Distribuida desarrollando cuatro aspectos que lo configuran: las Energías Renovables No Convencionales como fuente primaria, la Generación Distribuida y su impacto general, las Energías Renovables No Convencionales en un mercado eléctrico competitivo y los sistemas de comercialización o intercambio de energía en un esquema de Generación Distribuida. Posteriormente se configura un escenario básico evolutivo para la provincia de Córdoba, se determinan valores de Feed In Tariff y se analiza su eventual impacto en las tarifas de usuarios finales. Se efectúa un análisis de sensibilidad de las tarifas de energía y de los costos de instalación sobre la Feed In tariff.First The Distributed Generation concept is contextualized developing four aspects that make it up: Non-Conventional Renewable Energies as a primary source; Distributed Generation and their overall impact; Non-Conventional Renewable Energies in a competitive electricity market and marketing systems or energy exchange in DG scheme. Later an evolutionary basic scenario for the province of Córdoba is set, values FIT rates are determined and analyzed their possible impact on the tariff of end users. A sensitivity analysis of energy tariffs and installation costs on the Feed in Tariff is made.Tema 7: Uso eficiente y racional de la energía. Políticas y economía energética.Facultad de Arquitectura y Urbanism

La provincia de Córdoba en un escenario de generación distribuida bajo un sistema de feed in tariff

En primer lugar se contextualiza el concepto de Generación Distribuida desarrollando cuatro aspectos que lo configuran: las Energías Renovables No Convencionales como fuente primaria, la Generación Distribuida y su impacto general, las Energías Renovables No Convencionales en un mercado eléctrico competitivo y los sistemas de comercialización o intercambio de energía en un esquema de Generación Distribuida. Posteriormente se configura un escenario básico evolutivo para la provincia de Córdoba, se determinan valores de Feed In Tariff y se analiza su eventual impacto en las tarifas de usuarios finales. Se efectúa un análisis de sensibilidad de las tarifas de energía y de los costos de instalación sobre la Feed In tariff.First The Distributed Generation concept is contextualized developing four aspects that make it up: Non-Conventional Renewable Energies as a primary source; Distributed Generation and their overall impact; Non-Conventional Renewable Energies in a competitive electricity market and marketing systems or energy exchange in DG scheme. Later an evolutionary basic scenario for the province of Córdoba is set, values FIT rates are determined and analyzed their possible impact on the tariff of end users. A sensitivity analysis of energy tariffs and installation costs on the Feed in Tariff is made.Tema 7: Uso eficiente y racional de la energía. Políticas y economía energética.Facultad de Arquitectura y Urbanism

La provincia de Córdoba en un escenario de generación distribuida bajo un sistema de feed in tariff

En primer lugar se contextualiza el concepto de Generación Distribuida desarrollando cuatro aspectos que lo configuran: las Energías Renovables No Convencionales como fuente primaria, la Generación Distribuida y su impacto general, las Energías Renovables No Convencionales en un mercado eléctrico competitivo y los sistemas de comercialización o intercambio de energía en un esquema de Generación Distribuida. Posteriormente se configura un escenario básico evolutivo para la provincia de Córdoba, se determinan valores de Feed In Tariff y se analiza su eventual impacto en las tarifas de usuarios finales. Se efectúa un análisis de sensibilidad de las tarifas de energía y de los costos de instalación sobre la Feed In tariff.First The Distributed Generation concept is contextualized developing four aspects that make it up: Non-Conventional Renewable Energies as a primary source; Distributed Generation and their overall impact; Non-Conventional Renewable Energies in a competitive electricity market and marketing systems or energy exchange in DG scheme. Later an evolutionary basic scenario for the province of Córdoba is set, values FIT rates are determined and analyzed their possible impact on the tariff of end users. A sensitivity analysis of energy tariffs and installation costs on the Feed in Tariff is made.Tema 7: Uso eficiente y racional de la energía. Políticas y economía energética.Facultad de Arquitectura y Urbanism

Adsorption of peroxidase from Raphanus sativus L onto alginateguar gum matrix: Kinetic, equilibrium and thermodynamic analysis

This work explores the kinetics, equilibrium and thermodynamics of peroxidase adsorption onto spherical guar gumalginate matrices. The effect of contact time, solution pH, initial protein concentration and temperature was studied in batch experiments. The results show that peroxidase adsorption increased with rising contact time and initial enzyme concentration, and was higher at pH 4.0. The kinetic processes can be predicted by both the pseudo-first-order rate kinetics and the pseudo-second-order rate kinetics. Equilibrium adsorption data were analyzed with different isotherm models. The experimental data fitted to the Freundlich model in agreement with the low energy activation, demonstrating the presence of a high physical and unspecific interaction between the enzyme and the matrix.The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: FonCyT, Projects PICT 2013–271 – Argentina Innovator 2020 and Biotechnologies to Valorize the regional food Biodiversity in Latin America – Marie Curie Actions – IRSES Project number 611493 – European Community.info:eu-repo/semantics/publishedVersio

Inadequate BiP availability defines endoplasmic reticulum stress.

How endoplasmic reticulum (ER) stress leads to cytotoxicity is ill-defined. Previously we showed that HeLa cells readjust homeostasis upon proteostatically driven ER stress, triggered by inducible bulk expression of secretory immunoglobulin M heavy chain (μs) thanks to the unfolded protein response (UPR; Bakunts et al., 2017). Here we show that conditions that prevent that an excess of the ER resident chaperone (and UPR target gene) BiP over µs is restored lead to µs-driven proteotoxicity, i.e. abrogation of HRD1-mediated ER-associated degradation (ERAD), or of the UPR, in particular the ATF6α branch. Such conditions are tolerated instead upon removal of the BiP-sequestering first constant domain (CH1) from µs. Thus, our data define proteostatic ER stress to be a specific consequence of inadequate BiP availability, which both the UPR and ERAD redeem

Cell Cycle-Dependent Microtubule-Based Dynamic Transport of Cytoplasmic Dynein in Mammalian Cells

BACKGROUND:Cytoplasmic dynein complex is a large multi-subunit microtubule (MT)-associated molecular motor involved in various cellular functions including organelle positioning, vesicle transport and cell division. However, regulatory mechanism of the cell-cycle dependent distribution of dynein has not fully been understood. METHODOLOGY/PRINCIPAL FINDINGS:Here we report live-cell imaging of cytoplasmic dynein in HeLa cells, by expressing multifunctional green fluorescent protein (mfGFP)-tagged 74-kDa intermediate chain (IC74). IC74-mfGFP was successfully incorporated into functional dynein complex. In interphase, dynein moved bi-directionally along with MTs, which might carry cargos such as transport vesicles. A substantial fraction of dynein moved toward cell periphery together with EB1, a member of MT plus end-tracking proteins (+TIPs), suggesting +TIPs-mediated transport of dynein. In late-interphase and prophase, dynein was localized at the centrosomes and the radial MT array. In prometaphase and metaphase, dynein was localized at spindle MTs where it frequently moved from spindle poles toward chromosomes or cell cortex. +TIPs may be involved in the transport of spindle dyneins. Possible kinetochore and cortical dyneins were also observed. CONCLUSIONS AND SIGNIFICANCE:These findings suggest that cytoplasmic dynein is transported to the site of action in preparation for the following cellular events, primarily by the MT-based transport. The MT-based transport may have greater advantage than simple diffusion of soluble dynein in rapid and efficient transport of the limited concentration of the protein