Figure 1

This is the alignment of the sequences used for the MLSA analysis, these sequences are generated by concatenating 11 housekeeping genes as described in the publication. the and the tree alignment were performed using MEGA 6 software

Figure 4

This file contains the alignments and trees generated using MEGA6. the trees are presented in the figure 4 in the publication

La Petite presse : journal quotidien... / [rédacteur en chef : Balathier Bragelonne]

21 février 18681868/02/21 (A3,N673)

Bacterial QS signal producers or biosensors, and plasmids.

<p>Gm^r and Tet^r indicate resistance to gentamicin and tetracycline, respectively.</p><p>NAHSL, N-acyl homoserine lactones; AI-2, Autoinducer-2; HHQ, 4-hydroxy-2-heptylquinoline; PQS, Pseudomonas quinolone signal.</p

Kinetics of IAA-like production measured in the supernatant of potato soft-rot <i>Dickeya</i> spp.

<p>Indolic compounds were quantified by a colorimetric method with Salkowski's reagent. Bacterial growth was monitored by measuring optical density (OD) at 580 nm. For each point, at least 3 independent cultures in M9 minimal medium supplemented with l-tryptophan (500 µg/ml) were analyzed, with standard deviation shown.</p

Virulence traits and origin of potato soft-rot pathogens.

*<p>The potential of each strain to induce a tuber soft-rot was evaluated in potato host plant seven days after infection by intra-medulla injection.</p>#<p>The potential of each strain to induce a hypersensitive response (HR) was evaluated in tobacco non host plant 24 hours after leaves infiltration.</p><p>CFBP, Collection Française de Bactéries Phytopathogènes, Institut National de la Recherche Agronomique, Angers, France.</p

Characterization of signaling molecules produced by potato soft-rot pathogens.

*<p>Production of different N-acyl homoserine lactones (NAHSL) was determined by thin-layer chromatography (TLC) and HPLC-MS/MS. For quantification, NAHSL were extracted from PGA minimal medium culture at late exponential phase (optimal production).</p>#<p>Autoinducer-2 (AI-2) activity was determined using biosensor <i>V. harveyi</i> BB170.</p>°<p>Production of 4-hydroxy-2-heptylquinoline (HHQ) and Pseudomonas quinolone signal (PQS) were determined by TLC.</p>†<p>Production of indole-3-acetic acid (IAA), indole-3-propionic acid (IPA), indole-3-butyric acid (IBA) and kynurenic acid (KA) were determined by HPLC-UV. For quantification, indolic compounds were extracted from M9 minimal medium supplemented with l-tryptophan culture at stationary growth phase (optimal production).</p>§<p>Production of γ-amino butyric acid (GABA) was determined by ELISA test.(+) positive detection by the biosensor; (−) not detected or below threshold.</p

Kinetics of <i>metK</i>, <i>luxI</i>, <i>luxS</i> and <i>iaaM</i> gene expression involved in AI-1, AI-2 and IAA production by <i>D. chrysanthemi</i> strain CFBP 2048^T.

<p>AI-1 and AI-2 biosynthetic pathways are described in the legend to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0035176#pone-0035176-g003" target="_blank">Fig. 3</a>. A third signal, the indole-3-acetic acid (IAA) is synthesized by the indole-3-acetamine (IAM) pathway, in which l-tryptophan is first converted to IAM by the key enzyme of this pathway, the IaaM tryptophan-2-monooxygenase. IAM is then converted to IAA by IaaM hydrolase <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0035176#pone.0035176-Yang1" target="_blank">[36]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0035176#pone.0035176-Spaepen1" target="_blank">[54]</a>. Abundances of <i>metK</i> (checkered bars), <i>luxI</i> (white bars), <i>luxS</i> (black bars) and <i>iaaM</i> (grey bars) mRNAs were determined by RT-PCR experiments on RNA extracts from cells grown in PGA minimal medium (<b>A</b>) or M9 minimal medium supplemented with l-tryptophan (500 µg/ml) (<b>B</b>) and harvested at mid-exponential phase (<b>1</b>), late exponential phase (<b>2</b>), during the transition from exponential to stationary phases (<b>3</b>) or early stationary growth phase (<b>4</b>) followed by electrophoresis on 1% (m/v) agarose gels. Results were expressed as a ratio: synthase transcripts vs. 16S transcripts. The corresponding kinetics of AI-1 and AI-2 activities and IAA production in the supernatant of potato soft-rot <i>Dickeya</i> strain are shown at the top.</p