Expression systems for industrial Gram-positive bacteria with low guanine and cytosine content

Recent years have seen an increase in the development of gene expression systems for industrial Gram-positive bacteria with low guanine and cytosine content that belong to the genera Bacillus, Clostridium, Lactococcus, Lactobacillus, Staphylococcus and Streptococcus. In particular, considerable advances have been made in the construction of inducible gene expression systems based on the capacity of these bacteria to utilize specific sugars or to secrete autoinducing peptides that are involved in quorum sensing. These controlled expression systems allow for present and future exploitation of these bacteria as cell factories in medical, agricultural, and food biotechnology.

Extracting the top-quark running mass using ttˉt\bar{t}+1-jet events produced at the Large Hadron Collider

We present the calculation of the next-to-leading order QCD corrections for top-quark pair production in association with an additional jet at hadron colliders, using the modified minimal subtraction scheme to renormalize the top-quark mass. The results are compared to measurements at the Large Hadron Collider run I. In particular, we determine the top-quark running mass from a fit of the theoretical results presented here to the LHC data

Statistical analysis of time-resolved emission from ensembles of semiconductor quantum dots: Interpretation of exponential decay models

We present a statistical analysis of time-resolved spontaneous emission decay curves from ensembles of emitters, such as semiconductor quantum dots, with the aim of interpreting ubiquitous non-single-exponential decay. Contrary to what is widely assumed, the density of excited emitters and the intensity in an emission decay curve are not proportional, but the density is a time integral of the intensity. The integral relation is crucial to correctly interpret non-single-exponential decay. We derive the proper normalization for both a discrete and a continuous distribution of rates, where every decay component is multiplied by its radiative decay rate. A central result of our paper is the derivation of the emission decay curve when both radiative and nonradiative decays are independently distributed. In this case, the well-known emission quantum efficiency can no longer be expressed by a single number, but is also distributed. We derive a practical description of non-single-exponential emission decay curves in terms of a single distribution of decay rates; the resulting distribution is identified as the distribution of total decay rates weighted with the radiative rates. We apply our analysis to recent examples of colloidal quantum dot emission in suspensions and in photonic crystals, and we find that this important class of emitters is well described by a log-normal distribution of decay rates with a narrow and a broad distribution, respectively. Finally, we briefly discuss the Kohlrausch stretched-exponential model, and find that its normalization is ill defined for emitters with a realistic quantum efficiency of less than 100%.\ud \u

Limits on Lorentz violation from charged-pion decay

Charged-pion decay offers many opportunities to study Lorentz violation. Using an effective field theory approach, we study Lorentz violation in the lepton, W-boson, and quark sectors and derive the differential pion-decay rate, including muon polarization. Using coordinate redefinitions we are able to relate the first-generation quark sector, in which no bounds were previously reported, to the lepton and W-boson sector. This facilitates a tractable calculation, enabling us to place bounds on the level of $10^{-4}$ on first-generation quark parameters. Our expression for the pion-decay rate can be used to constrain Lorentz violation in future experiments.Comment: 12 pages, 1 figure, Accepted for publication in Phys. Rev.

Theory of electron-hole asymmetry in doped {\em CuO2_2} planes

The magnetic phase diagrams, and other physical characteristics, of the hole- doped {\em La$_{2-x}$Sr$_x$CuO$_4$} and electron-doped {\em Nd$_{2-x}$Ce$_x$ CuO$_4$} high-temperature superconductors are profoundly different. Starting with the $t-t^{\prime}-J$ model, the spin distortions and the spatial distri- bution of carriers for the multiply-doped systems will be related to the diffe- rent ground states' single-hole quasiparticles. The low doping limit of the hole-doped material corresponds to $\vec k = (\pi/2,\pi/2)$ quasiparticles, states that generate so-called Shraiman-Siggia long-ranged dipolar spin distor- tions via backflow. We propose that for the electron-doped materials the single- hole ground state corresponds to $\vec k = (\pi,0)$ quasiparticles; we show that the spin distortions generated by such carriers are short-ranged. Then, we demonstrate the effect of this single-carrier difference in many-carrier ground states via exact diagonalization results by evaluating $S(\vec q)$ for up to 4 carriers in small clusters. Also, the different single-carrier quasiparticles generate important differences in the spatial distributions: for the hole-doped material the quasiparticles tend to stay far apart from one another, whereas for the electron-doped material we find tendencies consistent with the clustering of carriers, and possibly of low-energy fluctuations into an electronic phase separated state. Lastly, we propose the extrapolation of an approach based on the $t-t^{\prime}-J$ model to the hole-doped 123 system.Comment: 27 pages, revtex 3.0, 6 Postscript Figures; to be published in Phys. Rev. B, Nov. 1, 199

Modulating active sites in MOFs for improved Lewis acid or base catalysis

International audienc

Optimal control of light propagation through multiple-scattering media in the presence of noise

We study the control of coherent light propagation through multiple-scattering media in the presence of measurement noise. In our experiments, we use a two-step optimization procedure to find the optimal incident wavefront. We conclude that the degree of optimal control of coherent light propagation through a multiple-scattering medium is only determined by the number of photoelectrons detected per single speckle spot. The prediction of our model agrees well with the experimental results. Our results offer opportunities for imaging applications through scattering media such as biological tissue in the shot noise limit

3D spatially-resolved optical energy density enhanced by wavefront shaping

We study the three-dimensional (3D) spatially-resolved distribution of the energy density of light in a 3D scattering medium upon the excitation of open transmission channels. The open transmission channels are excited by spatially shaping the incident optical wavefronts. To probe the local energy density, we excite isolated fluorescent nanospheres distributed inside the medium. From the spatial fluorescent intensity pattern we obtain the position of each nanosphere, while the total fluorescent intensity gauges the energy density. Our 3D spatially-resolved measurements reveal that the local energy density versus depth (z) is enhanced up to 26X at the back surface of the medium, while it strongly depends on the transverse (x; y) position. We successfully interpret our results with a newly developed 3D model that considers the time-reversed diffusion starting from a point source at the back surface. Our results are relevant for white LEDs, random lasers, solar cells, and biomedical optics