16 research outputs found
Quantitative subproteomic analysis of germinating related changes in the scutellum oil bodies of Zea mays
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Use of CACTA mobile genetic elements for revision of phylogenetic relationships in Brassica rapa L. species
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Integration of retrotransposons-based markers in a linkage map of barley
A deeper understanding of random markers is important if they are to be employed for a range of
objectives. The sequence specific amplified polymorphism (S-SAP) technique is a powerful genetic analysis
tool which exploits the high copy number of retrotransposon long terminal repeats (LTRs) in the plant
genome. The distribution and inheritance of S-SAP bands in the barley genome was studied using the
Steptoe · Morex (S · M) double haploid (DH) population. Six S-SAP primer combinations generated 98
polymorphic bands, and map positions were assigned to all but one band. Eight putative co-dominant loci
were detected, representing 16 of the mapped markers. Thus at least 81 of the mapped S-SAP loci were
dominant. The markers were distributed along all of the seven chromosomes and a tendency to cluster was
observed. The distribution of S-SAP markers over the barley genome concurred with the knowledge of
the high copy number of retrotransposons in plants. This experiment has demonstrated the potential for the
S-SAP technique to be applied in a range of analyses such as genetic fingerprinting, marker assisted
breeding, biodiversity assessment and phylogenetic analyses