26 research outputs found
Current perspectives for engineering antimicrobial nanostructured materials
Pathogenic microorganisms are becoming a global health issue. Bacterial adhesion and growth on an implant surface form biofilms, endangering the fate of biomaterial in the body. Local infection from the infected implant increases patient mortality. Antibiotic-resistant bacteria have necessitated the development of new antibiotic generations. Nanotechnology is a growing field of science that has the potential to create new antibacterial materials. This concise review focuses on several new emerging antimicrobial areas: nanostructured surfaces/nanoparticles, polymer conformations, and two-dimensional antibacterial nanomaterials. Traditional antimicrobial drugs can be triggered by smart stimuli like the environments (pH, moisture, etc.) or physical stimulation like magnetic field and light. A special focus is devoted to the most recent advances in liquid metal particles that can be activated by external stimuli. Conformations of antibacterial polymers have also caught researcher interest owing to their unique bactericidal processes. The review concludes with the authorsâ vision for the future directions of the field
NatureâInspired Biomimetic Surfaces for Controlling Bacterial Attachment and Biofilm Development
Abstract The use of antibacterial and antifouling materials is widely being investigated to combat the increasing risk associated with bacterial infections and the evolution of drugâresistant bacteria. Efficient antibacterial materials can be fabricated by mimicking the topography found on the surface of natural antibacterial materials. Natural materials such as the wings of cicadas and dragonflies have evolved to use the structural features on their surface to attain bactericidal properties. The nanopillars/nanospikes present on these natural materials physically damage the bacterial cells that settle on the nanostructures resulting in cell lysis and death. This article reviews the role of nanostructures found on the surface of some of these natural antibacterial and antifouling materials such as lotus leaf, cicadas and dragonflies wings, shark skin, and rose petals. These natural structures provide guidelines for the design of synthetic bioâinspired materials. This review article also presents some novel fabrication techniques used to produce biomimetic microâ and nanoâstructures on synthetic material surfaces. The role of size, shape, aspect ratio, and spacing between the micro/nanoâstructures on the bactericidal properties is also discussed. Finally, the review is finished with the author's view on the future of the field
Antibacterial Longevity of a Novel Gallium Liquid Metal/Hydroxyapatite Composite Coating Fabricated by Plasma Spray
Hydroxyapatite (HAp)-coated metallic implants are known for their excellent bioactivity and osteoconductivity. However, infections associated with the microstructure of the HAp coatings may lead to implant failures as well as increased morbidity and mortality. This work addresses the concerns about infections by developing novel composite coatings of HAp and gallium liquid metal (GaLM) using atmospheric plasma spray (APS) as the coating technique. Five weight percent Ga was mixed into a commercially supplied HAp powder using an orbital shaker; then, the HAp-Ga particle feedstock was coated onto Ti6Al4V substrates using the APS technique. The X-ray diffraction results indicated that Ga did not form any Ga-related phases in either the HAp-Ga powder or the respective coating. The GaLM filled the pores of the HAp coating presented both on the top surface and within the coating, especially at voids and cracks, to prevent failures of the coating at these locations. The wettability of the surface was changed from hydrophobic for the HAp coating to hydrophilic for the HAp-Ga composite coating. Finally, the HAp-Ga coating presented excellent antibacterial efficacies against both initial attachments and established biofilms generated from methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa after 18 h and 7 days of incubation in comparison to the control HAp coating. This study shows that GaLM improves the antibacterial properties of HAp-based coatings without sacrificing the beneficial properties of conventional HAp coatings. Thus, the HAp-Ga APS coating is a viable candidate for antibacterial coatings
Biomimetic Bacterium-like Particles Loaded with Aggregation-Induced Emission Photosensitizers as Plasma Coatings for Implant-Associated Infections
Developing novel antibacterial strategies has become
an urgent
requisite to overcome the increasing pervasiveness of antimicrobial-resistant
bacteria and the advent of biofilms. Aggregation-induced emission-based
photosensitizers (AIE PSs) are promising candidates due to their unique
photodynamic and photothermal properties. Bioengineering structure-inherent
AIE PSs for developing thin film coatings is still an unexplored area
in the field of nanoscience. We have adopted a synergistic approach
combining plasma technology and AIE PS-based photodynamic therapy
to develop coatings that can eradicate bacterial infections. Here,
we loaded AIE PSs within biomimetic bacterium-like particles derived
from a probiotic strain, Lactobacillus fermentum. These hybrid conjugates are then immobilized on polyoxazoline-coated
substrates to develop a bioinspired coating to fight against implant-associated
infections. These coatings could selectively kill Gram-positive and
Gram-negative bacteria, but not damage mammalian cells. The mechanistic
studies revealed that the coatings can generate reactive oxygen species
that can rupture the bacterial cell membranes. The mRNA gene expression
of proinflammatory cytokines confirmed that they can modulate infection-related
immune responses. Thus, this nature-inspired design has opened a new
avenue for the fabrication of a next-generation antibacterial coating
to reduce infections and associated burdens
Interactions between Liquid Metal Droplets and Bacterial, Fungal, and Mammalian Cells
Liquid metals (LMs) have emerged as novel materials for biomedical applications. Here, the interactions taking place between cells and LMs are reported, presenting a unique opportunity to explore and understand the LM-biological interface. Several high-resolution imaging techniques are used to characterize the interaction between droplets of gallium LM and bacterial, fungal, and mammalian cells. Adhesive interactions between cells and LM droplets are observed, causing deformation of the LM droplet surface, resulting in surface wrinkling and in some cases, breakage of the native oxide layer present on the LM droplet surface. In many instances, the cell wall deforms to intimately contact the LM droplets. Single-cell force spectroscopy is performed to quantify the adhesion forces between cells and LM and characterize the nature of the adhesion. It is proposed that the flexible nature of the cell enables multiple adhesion sites with the LM droplets, imparting tensile forces on the LM droplet surface, which results in surface wrinkling on the LM droplets due to their liquid nature. Molecular dynamics simulations also suggest that flexible biomolecules on the cell surface can disrupt the Ga2O3 layer formed at the LM droplet surface. This study reveals a unique biointerfacial interaction and provides insights into the mechanisms involved
The photocatalytic decomposition of chloroform by tetrachloroaurate(III)
Near-UV irradiation of solutions of (Bu4N)AuCl4 in aerated ethanol-stabilized chloroform causes the continuous decomposition of chloroform, as evidenced by the production of many equivalents of HCl and peroxides. At the outset of irradiation, most of the AuCl4 â is reduced to AuCl2 â, but the reduction stops and is reversed. The same experiments done in ethanol-free chloroform cause chloroform decomposition only until the irreversible reduction of the gold is complete. In deoxygenated ethanol-free chloroform, irreversible reduction to AuCl2 â is accompanied by the formation of HCl and CCl4, while the main decomposition products in deoxygenated ethanol-stabilized chloroform are HCl and C2Cl6. It is proposed that, in ethanol-free chloroform, photoreduction of AuCl4 â begins with the concerted elimination of HCl from an association complex of CHCl3 with AuCl4 â, and that ethanol suppresses{CHCl3â
AuClâ4}{CHCl3â
AuCl4â} complex formation, leaving a slower radical process to carry out the photoreduction of AuCl4 â in ethanol-stabilized chloroform. In the presence of oxygen, the radical process causes a build-up of CCl3OOH, which reoxidizes AuCl2 â to AuCl4 â and allows the photodecomposition of CHCl3 to continue indefinitely