101 research outputs found
Establishing cost-effectiveness of genetic targeting of cancer therapies\ud
The clinical benefit of a new genomic instrument, the 70-gene signature\ud
for breast cancer patients, is being evaluated in a randomised clinical\ud
trial. The early, controlled implementation process is supported by a\ud
Constructive Technology Assessment to help decision-making in an\ud
uncertain time of developmen
The promise of microarrays in the management and treatment of breast cancer
Breast cancer is the most common malignancy afflicting women from Western cultures. Developments in breast cancer molecular and cellular biology research have brought us closer to understanding the genetic basis of this disease. Recent advances in microarray technology hold the promise of further increasing our understanding of the complexity and heterogeneity of this disease, and providing new avenues for the prognostication and prediction of breast cancer outcomes. These new technologies have some limitations and have yet to be incorporated into clinical use, for both the diagnosis and treatment of women with breast cancer. The most recent application of microarray genomic technologies to studying breast cancer is the focus of this review
Microarrays and breast cancer clinical studies: forgetting what we have not yet learnt
This review takes a sceptical view of the impact of breast cancer studies that have used microarrays to identify predictors of clinical outcome. In addition to discussing general pitfalls of microarray experiments, we also critically review the key breast cancer studies to highlight methodological problems in cohort selection, statistical analysis, validation of results and reporting of raw data. We conclude that the optimum use of microarrays in clinical studies requires further optimisation and standardisation of methodology and reporting, together with improvements in clinical study design
Microarrays de DNA en el cáncer oral
Uno de los principales objetivos en la investigación sobre el
cáncer en la actualidad es el estudio de marcadores que puedan
predecir el pronóstico o la respuesta al tratamiento de forma
individual. El número de genes implicados en los distintos pasos
de la carcinogénesis oral aumenta a medida que se investiga
sobre el tema. Los microarrays de DNA permiten el análisis
simultáneo de la expresión de cientos de genes de un tejido en
un solo experimento. El formato paralelo del ensayo permite el
estudio de diferencias en la expresión genética entre células
normales y enfermas, puesto que la actividad de cada gen en el
microarray puede ser comparada en dos poblaciones celulares
distintas. El objetivo de este trabajo es hacer una breve revisión
de los estudios realizados por diversos autores que han intentado
identificar genes relacionados con el cáncer oral, asà como
clasificarlo en subgrupos según los patrones de expresión
genética; lo que permitirá una precoz detección, mejor diagnóstico
y pronóstico del cáncer oral.One of the principal aims of modern cancer research is to identify
markers allowing individual prediction of prognosis or response
to treatment. In this connection, the number of genes thought
to be involved in the different stages of different types of oral
cancer increases apace. DNA microarrays allow simultaneous
evaluation of the expression of hundreds of genes in a single
assay. The parallel format of microassay slides is designed to
allow rapid comparison of gene expression between two
samples, for example tumor cells and healthy cells. This article
reviews studies that have aimed to identify genes related to oral
cancer, and to classify these genes into groups that are commonly
co-expressed. These studies suggest that DNA microarrays are
set to become routine tools in the detection, diagnosis,
characterization and treatment of oral cancers
Identification of Novel Variants of Metadherin in Breast Cancer
Metadherin (MTDH, also known as AEG-1, and Lyric) has been demonstrated to play a potential role in several significant aspects of tumor progression. It has been reported that overexpression of MTDH is associated with progression of disease and poorer prognosis in breast cancer. However, there are no studies to date assessing variants of the MTDH gene and their potential relationship with breast cancer susceptibility. Thus, we investigated all variants of the MTDH gene and explored the association of the variants with breast cancer development. Our cohort consisted of full-length gene sequencing of 108 breast cancer cases and 100 healthy controls; variants were detected in 11 breast cancer cases and 13 controls. Among the variants detected, 9 novel variants were discovered and 2 were found to be associated with the susceptibility of breast cancer. However, additional studies need to be conducted in larger sample sizes to validate these findings and to further investigate whether these variants are prognostic in breast cancer patients
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