ANALYSIS OF METHODS TO INCREASE BRAND LOYALTY AMONG RUSSIAN TELEVISION CHANNELS

Based on the study of marketing activities of Russian TV channels of entertainment and educational segments the modern methods of attracting viewers to television brands have been analyzed. The objects of the study are seven well-known public broadcasters, whose business has established more than twenty-five significant and cost-effective ways to influence the loyalty of the audience. In combination with negative factors indirectly affecting the implementation of loyalty programs, taking into account the existing methods of other industries, a minimum independent evaluation of the effectiveness of loyalty, which can serve as an idea for new marketing developments, and will contribute to the development of scientific research in this economical area, has been suggested

Pharmacological evaluation polysaccharide complex flowers tansy

From flowers tansy extracted polysaccharide complex. Installed its qualitative and quantitative composition, have developed a technique standardizing the content of reducing sugars. By thin layer chromatography and high pressure liquid chromatography after acid hydrolysis installed monosaccharide composition: glucose, xylose, arabinose, galactose and mannose. It is proved that the polysaccharide has a high content of uronic acid, which allows it to include the class of pectin. The investigation of gastroprotective activity of polysaccharide in the prophylactic administration at model destruction of the gastric mucosa to indomethacin. Introduction polysaccharide prevents various types of erosive and ulcerative destruction. According to anti-ulcer activity of the drug is superior to ranitidine and comparable to omeprazol

How Nursing Affects Medicare’s Outcome-Based Hospital Payments

Improving value is one of the central aims of recent and ongoing health care reform. In our last LDI/INQRI Brief, we reviewed the evidence of the role of nurses in increasing the value of health care. In this companion brief, we dig deeper into the three reimbursement strategies that Medicare uses to align hospital financial incentives with quality of care, and we calculate the potential effects of nursing-sensitive quality indicators on hospital payments

Phraseological-Paroemic Description of the French Political Elite

В статье рассматривается модель формирования речевого имиджа современной политической элиты Французской Республики через анализ интерпретативной составляющей политического дискурса.The article examines the phenomenon of political malapropisms - statements of politicians which have come to the attention of the media and the public opinion. Such malapropisms, interpreted as phraseological-parоemic units of political discourse, constitute, in the author's view, the subject of political phraseology and paroemiology. The article provides their functional typological and characterological description in accordance with the principle of communicative relevance, comism and adequacy of speech deontology

Increasing the Value of Health Care: The Role of Nurses

Increasing health care value has become a central objective of payment policies, insurance design and purchasing, and patient and provider decision-making. The word “value” appeared in the title of seven sections of the Affordable Care Act (ACA), and earlier this year CMS set a goal of having 50 percent of reimbursement based on value. This brief reviews nurses’ contribution to value, highlighting evidence published by researchers in the Interdisciplinary Nursing Quality Research Initiative (INQRI), an 8-year program funded by the Robert Wood Johnson Foundation. It also looks at interventions designed to address outcomes targeted by ACA- or Medicare-related payment policies

Ecogeographic study of peanut accessions from the VIR collection

Background. It has been shown that southern oilseeds, including peanuts, can be successfully grown in the south of the Russian Federation under the conditions of Krasnodar Territory and Astrakhan Province. Currently, only two peanut cultivars are included in the State Register of the Russian Federation: ‘Otradokubansky’ and ‘Astrakhansky 5’. Development of raw material for obtaining new peanut cultivars adaptable to the growing conditions in Krasnodar Territory and Astrakhan Province is a relevant task.Materials and methods. We examined 57 peanut accessions of various origins from the VIR collection. The study of peanut accessions for agronomic characters was carried out in two ecogeographic sites located in Krasnodar Territory and Astrakhan Province. Cv. ‘Otradokubansky’ was used as a reference. ANOVA was applied for statistical data processing.Results and discussion. A wide range of variability in agronomic characters was revealed during the study of peanut accessions. The most stable character was the 1000 seed weight. The share of the genotype the variability of productivity was from 30 to 40%. A great effect of soil and climate factors and the place of cultivation on the ripening of beans was observed. Seed yield depended on the genotype (60–70%) as well as on the temperature factors and the presence of moisture in the soil. The best accessions were selected according to their agronomic characters; they may serve as promising material for breeding. Some accessions are able to produce a good harvest either in Astrakhan Province or Krasnodar Territory, while others can do it regardless of the place of cultivation. This study confirmed the possibility of growing peanuts in Astrakhan Province on medium loamy soils under irrigation, or in Krasnodar Territory on black earth without irrigation

Biological features and cultivation of sesame (a review)

Sesame is an ancient oilseed crop grown throughout the arid and subarid climates. Sesame oil has a good taste and is appreciated for its beneficial properties. The genus Sesamum L. (Pedaliaceae Lindl.) includes up to 38 species. The only cultivated species is S. indicum L. (2n = 26). It is grown in areas located between 45° N and 45° S. South Africa is considered the homeland of S. indicum. Sesame seeds contain 50–63% of oil, 25–27% of protein, 20% of carbohydrates, and are sources of such micronutrients as copper, calcium, iron, magnesium, zinc, vitamins A, B1, C and E. Sesame seeds and oil are used not only in the food industry but also in medicine. Sesame meal is a good animal feed. Recently, thanks to the deciphering of the sesame nuclear genome and the development of various genomic resources, including molecular markers for qualitative and quantitative characters, molecular breeding has become possible for such agronomic traits as high oil content and its composition, drought resistance, resistance to waterlogging, disease resistance, and high yield. The world production of sesame seeds according to FAO estimates for 2019 was about 7 million tons. The largest cropping areas are located in Africa, India, and China. In Russia, sesame can be grown in southern regions: Krasnodar and Stavropol Territories, Rostov and Astrakhan Provinces. Breeding work on sesame was carried out at the All-Union Research Institute of Oil Crops, where cultivars resistant to bacterial blight and Fusarium wilt were developed. Currently, sesame breeding programs are absent in Russia. There are over 25,000 sesame accessions in the world’s genebanks, including about 1,500 accessions in the VIR collection

The Role of Vesicles in Transporting of Cholera Toxin

The review reports on the secretion pathways of the main virulence factor of Vibrio cholerae, cholera toxin, both through the two-stage Sec-dependent type 2 secretion system and with the help of vesicles of the outer membrane of V. cholerae. The ways of toxin transfer into the host organism, depending on its form, are discussed. The well-studied free soluble cholera toxin is secreted extracellularly and transmitted in a GM1-dependent manner through cholesterolrich lipid rafts. The transfer of cholera toxin associated with vesicles has advantages over free toxin, because substances inside the outer membrane vesicles are protected from external proteases and host antibodies by the membrane that forms the vesicle. Vesicular transporting of cholera toxin into the target cell occurs via clathrin-dependent, caveolin-dependent and lipid raft-dependent endocytosis. The specific transport route is determined by the structure of the vesicles. Clathrindependent endocytosis is described for V. cholerae strains cultivated at low osmolarity of the medium, whose outer membrane vesicles contain the cholera toxin subunit A inside. Lipid raft-dependent endocytosis is characteristic of vesicles in which cholera toxin is located on the surface. In addition, endocytosis of V. cholerae outer membrane vesicles through structures known as caveolae is presented

Mitoxantrone Quantification by HPLC-MS/MS in Caco-2 Culture Media

Mitoxantrone is a marker substrate of breast cancer resistance protein (BCRP). BCRP is involved in a number of pharmacokinetic drug-drug interactions. The transporter’s possible saturability makes it advisable to use low concentrations of mitoxantrone for in vitro studies. Consequently, mitoxantrone quantification requires   a method with high sensitivity.The aim of the study was to develop and validate a procedure for mitoxantrone quantification in Caco-2 culture media by HPLC-MS/MS.Materials and methods.  The  authors  used  an  Ultimate  3000  HPLC  system  and a TSQ Fortis triple quadrupole mass spectrometer by Thermo Fisher Scientific and a Selectra C18 column (4.6×100 mm, 5 μm, 100 Å) by United Chemical Technologies. The elution ran in a gradient mode with a mobile phase of 1% formic acid solution and methanol. Experimental parameters were as follows: eluent flow rate, 0.3 mL/min; separation column temperature, 35 °C; injection volume, 5  μL; ana lysis time, 10 min; approximate mitoxantrone retention time, 5.51 min. The sample preparation involved protein precipitation from the culture medium with methanol, followed by centrifugation at 13,000 g for 10 min. The detection was performed using electrospray ionisation in the positive ion mode. Detection parameters were   as follows: electrospray voltage, 3700 V; sheath gas flow rate, 50 L/min; auxiliary    gas flow rate, 10 L/min; sweep gas flow rate, 1 L/min; ion-transfer tube temperature, 300 °C; and evaporator temperature, 350 °C. The detection was set at mass transitions of m/z 455 to 88.2 and m/z 455 to 358.1, with the collision energy for these transitions amounting to 25 V and 18 V, respectively. The source fragmentation was at 0, and the CID gas pressure was at 2 mTorr.Results. The analytical procedure showed selectivity, high sensitivity (limit of detection, 10 nmol/L; lower limit of quantification, 50 nmol/L), accuracy, precision, and linearity in the concentration range of 50–1000 nmol/L. The authors observed no carryover or matrix effects. A simulation of real-life storage conditions demonstrated high stability of mitoxantrone samples. Thus, the analytical procedure enables preclinical evaluation of medicinal product effects on the functional activity of BCRP, based on assessing the transcellular mitoxantrone transport in the presence of a test product.Conclusion. The authors developed and validated the analytical procedure for mitoxantrone quantification in Caco-2 culture media by HPLC-MS/MS