Duration of senescent cell survival in vitro as a characteristic of organism longevity, an additional to the proliferative potential of fibroblasts

AbstractMore than 40 years have passed since the original publication by Hayflick and Moorhead led to the concept of the ‘Hayflick limit’ of the maximum number of divisions which somatic cells undergo in vitro. This concept is still regarded as a fundamental characteristic of species longevity. Here we want to emphasize another characteristic of somatic cells, namely, the duration of their survival in vitro in the non-dividing state after cessation of proliferation. This is suggested on the basis of results of recent experiments with so-called Japanese accelerated senescent mice. Results of these experiments reveal a good correlation between the longevity of the mice, the number of duplications of their fibroblasts in vitro, and the survival time of these cells in the non-dividing state. In routine culture conditions, cell survival time may be very long, as much as a few years. However, when the cells are grown under conditions of oxidative stress, cellular longevity is markedly shortened. This new test may serve as an additional marker of organismic longevity. The comparative value of both tests, the classical ‘Hayflick limit’ and the new test, is discussed

Using corrugation stitch in submammary fold at the reconstructive breast surgery

The aim of the research was to develop a new technique for suturing the surgical incision in submammary area after subpectoral reconstructive breast surgery with good cosmetic result. Clinical observation group included 10 patients. It was found that the new technique of suturing the surgical incision, in comparison with the method offered by M.B. Calobrace et al. (2014), allows to decrease the duration of suturing from 6 min 10 ± 0.06 sec down to 5 min 29 ± 0.04 sec (р < 0.01), to reduce number of stitches from 24 down to 15, to shorten postoperative stitch from 4.24 ± 0.06 cm down to 3.71 ± 0.07 cm (р < 0.01), and to shorten cicatrix length in 1 year after the operation from 4.78 ± 0.19 cm down to 4.25 ± 0.22 cm (р < 0.05). Therefore, the developed technique of suturing the surgical wound provides the location of postoperative cicatrix in the area of submammary fold which makes it less noticeable. Moreover, it allows to shorten the length of postoperative cicatrix, decrease the duration of the surgery and to secure adequate stability of implant fixation

EXPERIENCE TREATMENT OF CHILDREN WITH POST-BURN SCAR CONTRACTURE OF THE NECK

Cicatricial deformations of the neck occur in 4-30.5 % of children suffered from burn trauma, and are the most severe consequences. Early reconstructive surgery in this pathology provides free growth and development of the skull and upper body and promote psychological and social recovery of the child. The treatment of such patients presents great difficulties, and in each case requires an individual approach. The article presents the experience of treatment of two children with severe scar contracture of the neck, complicated by the lack of reserves in undamaged tissues of the neck and the surrounding areas. We used a combination of several methods of skin grafting, including axial flow and the prefabrication of flaps, divided in time into stages. In one case in a child with total neck contracture, we performed pre-surgical (3.5 weeks before the main stage surgical intervention) preparation of the two flaps - scapular and epaulet flaps. In the other case, 4-year old girl with subtotal flexion contracture, a month before the main surgery we prepared scapular flap and increased its area expander dermotension, with endoscopic implantation of endoexpander. In this paper, we describe in detail the technology of operative treatment of these two patients. In both cases, there were no postoperative complications. The use of modern medical technologies based on conventional techniques in surgical correction of severe flexion scar contractures of the neck of the presented patients decided both functional and aesthetic problems

КОНТРОЛЬ ОСТАТОЧНЫХ НАПРЯЖЕНИЙ В СТРУКТУРАХ SI-SIO2

The consideration is made of the ways leading to increasing the control of residual stresses in structures such as silicon dioxide-monocrystal silicon substrate. The results concerning the determination of the level of residual stresses in structures Si-SiO2 are presented.Рассмотрены пути повышения точности контроля величины остаточных напряжений в структурах диоксид кремния-монокристаллическая кремниевая подложка. Приведены результаты определения уровня остаточных напряжений в структурах Si-SiO2

Dynamic tests of multi-detector radiometric system on central blood circulation phantom

Phantoms of organs and biological systems are widely used for verification of radioisotope protocols. The construction and operation of the heart and circulation phantom is described. The present model was designed for experimental testing of multi-detector radiometric system, prototype of which was designed in Ural Federal University as an extension and alternative to Anger camera and single-photon-emission tomography radionuclide diagnostics. The model represents vital dynamic properties of the single-chamber pulsing heart and circulation, featuring adjustable heart rate, blood volume, ejection fraction and flowrate. The trial experiments were performed with 99m Tc-pertechnetate to obtain time-activity curves and the ejection fraction was thus measured basing on first-pass radio angiography technique. © Published under licence by IOP Publishing Ltd

Permeability of Magnetic Cores with Air Gaps

The influence of the geometric dimensions of the cut core and the number and size of air gaps on the effective permeability was investigated. Using dimensional analysis, an equation was obtained that relates the permeability of the cut core to the simplest dimensionless combination of the mean magnetic flux length l, single air gap length lg1, the cross-sectional area S of the core, and gap number ng . Permeability calculated from the geometric parameters of the cut core was compared with the effective permeability obtained using a two-dimensional FEMM simulation. Simulation has shown that the equation derived from dimensional analysis provides the best fit. The influence of each significant parameters l, lg1, ng, and S on cut core permeability is demonstrated. Experimental results have shown that an equation derived from dimensional analysis can be used to predict cut core permeability. In this case, one should take into account the residual air gap that forms after cutting the core. A method for assessing the residual air gap is proposed. © 2022 by the authors. Licensee MDPI, Basel, Switzerland

Developing the structure of the personality's ecological culture

The article is devoted to a detailed analysis of the personality's ecological cultureand the description of its components. The author systematizes the elements of ecological culture, selected and described by modern researchers in the field of forming ecological culture and environmental consciousness of the person, systematizes them and establishes a functional relationship between them. The author considers the implementation of personal ecologically oriented activities to be the main purpose of forming ecological culture; therefore, the author proposes to enlarge the structure of ecological culture under consideration with such elements, as expert opinions and scientific environmental assessment (expert review). Copyright@ EM International

СЕЛЕКЦИОННОЕ ИСПОЛЬЗОВАНИЕ ГЕНЕТИЧЕСКИ СЛОЖНО НАСЛЕДУЕМОГО ПРИЗНАКА ФИОЛЕТОВОЙ ОКРАСКИ КОРНЕПЛОДОВ РЕДИСА

The understanding the nature of trait inheritance in any crops is that determines the quality of results in breeding program. According to reference on previous publication, it is known that phenotypic manifestation of purple root color in radish was caused by regulatory interrelationship mechanisms of genetic control that is difficult to be used directly in breeding program. From literature sources and on the basis of their own research work the authors have proven the practice to maintain the trait in generations, and implementations of development of purple radish breeding accessions have been presented. At first stage of breeding program the selection of initial breeding accessions was carried out, where 14 varieties (red x white) were regarded on the basis of top-crosses to obtain F1 and F2 progenies to be analyzed. Thus, four best combinations from crossing were chosen with 100% of hybridity. Through analysis of hybrids for individual progenies the hybrid population F1 of radish ‘Konfeti’ with different root colors was developed. As result of the individual inbreeding selection on seed plants with pigmented stems and the colored flower rim, the stable breeding accession with purple root was obtained. Thus, in breeding practice in radish it was succeeded to obtain the stably inheriting purple root color in radish accessions, variety ‘Siniiy Iniey’. Знание природы наследования признака в селекции любой культуры определяет качество селекционной работы с ней. Известно, что фенотипическое проявление фиолетовой окраски корнеплода редиса обусловлено регуляторными механизмами сложного генетического взаимодействия, трудно поддающегося селекционному использованию. На основе литературных источников и собственных селекционных исследований авторами обоснована практика закрепления признака в поколениях размножения и показаны примеры получения форм редиса с фиолетовой окраской корнеплодов. На начальном этапе селекционной работы с культурой была организована работа по подбору сортов для гибридизации методом топ-кросса 14 сортов (красный х белый), затем была проведена оценка F1, F2 и других поколений, были определены 4 лучших комбинации скрещивания со 100% гибридностью. На основе гибридологического анализа индивидуальных потомств нами была составлена гибридная популяция редиса Конфетти F1 c разноцветным окрашиванием корнеплодов. В результате индивидуального инбредного отбора по семенному растению (пигментация стеблей, окрашивание венчика цветка) получена стабильно наследующая форма редиса с фиолетовым окрашиванием корнеплода. Таким образом, в практике ведения селекционной работы с культурой редиса удалось получить стабильно наследуемую форму с фиолетовым окрашиванием корнеплода – сорт Синий иней

A portable radiometric system for nuclear medicine

It's shown suggested methods for diagnosing pathological processes in biological objects using a developed portable radiometric system with four gamma-ray detectors. Designed portable radiometric system was used to make 2D imaging of anatomical structures. The system can value pharmacokinetics in several points of wide area of interest at the patient body. In this study specially designed phantoms were used. Phantom models of the liver and blood circulation system were made to assay radiometric system. The construction concept and possible applications of the system in the practice of radionuclide diagnostic studies in vivo are presented. © 2018 Elsevier Lt