Multilevel D-loop PCR identification of hunting game

AbstractThe control region of mtDNA (D-loop) was used for hair samples of the five hunting game species identification: red deer (Cervus elaphus), roe deer (Capreolus capreolus), fallow deer (Dama dama), mouflon (Ovis aries musimon), and wild boar (Sus scrofa). For D-loop multilevel PCR detection scheme was applied in six primers (CE CVZV 1=5′-GATCACGAGCTTGATCACCA-3′; CE CVZV 2=5′-AGGAGTGGGCGATTTTAGGT-3′; DD CVZV 3=5′-CGCGTGAAACCAACAACCCGC-3′; DD CVZV 4=5′-CCGGGTCGGGGCCTTAGACG-3′; SSW CVZV 5=5′-ACACGTGCGTACACGCGCATA-3′; SSW CVZV 6=5′-GGTGCCTGCT T TCGTAGCACG-3′) designed to identify unknown biological samples of the hunting game animals. The PCR reaction volume was 25μl at conditions 95°C for 2min, 94°C for 30s, 60°C for 30s, 72°C for 30s, 35cycles, with last extension at 72°C for 10min. D-loop mtDNA amplicons of the game animals are characterized with specific PCR product sizes depending on species: red deer=163bp and 140bp, fallow deer=280bp and 138bp, roe deer=303bp, 280bp, 160bp and 138bp, mouflon=299bp and 178bp, wild boar=137bp and 229bp

Decrease in C-reactive protein levels in rabbits after vaccination with a live attenuated myxoma virus vaccine

The aim of this study was to evaluate the acute phase reaction and immune response of rabbits triggered by vaccination with a live attenuated myxoma virus (MXT) vaccine. Thirteen adult and 11 juvenile New Zealand white rabbit-based crossbreed rabbits, were used. Samples of rabbit peripheral blood were collected from vena auricularis centralis into heparinised tubes before vaccination and 48 h after vaccination. All animals were vaccinated by subcutaneous injection (0.5 ml) with a MXT vaccine. The blood plasma C-reactive protein level was measured by an ELISA kit using a double-antibody sandwich. For phenotyping of lymphocytes the fresh cells were stained with the following anti-rabbit monoclonal antibodies: anti-IgM, anti-CD4, anti-CD8 and anti-pan T2. Our results show that the use of attenuated myxoma virus vaccine significantly decreases the level of C-reactive protein in blood plasma of adult rabbits by 38.14% (P < 0.05) and of juvenile rabbits by 37.63% (P < 0.001), within 48 h. The rabbit C-reactive protein after MXT vaccination is a negative acute phase protein. In the group of adult rabbits the immune response to MXT vaccination was accompanied by a non-significant decrease in CD4+, pT2+, IgM+ subsets. On the other hand the values of CD8+, CD4+CD8+ and CD4+/CD8+ were non-significantly higher after MXT vaccination

Effective generation of genetically modified rabbits by sperm mediated gene transfer

[EN] Many reports in the past decade showing successful sperm mediated gene transfer (SMGT) offoreign DNA into both non-mammalian and mammalian animals. Fresh collected, extender washed rabbit spermcells were co-cultured with linear 14.3 kb gene construct and used for AI. Transgene integration in rabbitgenome was verified by PCR analysis. The PCR results show that transgenic founder rabbits transmittedtransgene to F1 generation. Our preliminary results suggest that SMGT could be an effective method forproduction of genetically modified rabbits.We would like to thank Mr. Jan Pecho and Dipl. Ing. Ivan Chlebec for taking care of the animals and for providing technical assistance. This work was financially supported by Ministry of Agriculture Slovak Republic and Slovak Research and Development Agency under the contract No. APVV-27-005505.Vasicek, D.; Vasickova, K.; Parkanyi, V.; Rafay, J.; Ondruska, L. (2007). Effective generation of genetically modified rabbits by sperm mediated gene transfer. World Rabbit Science. 15(3). https://doi.org/10.4995/wrs.2007.593SWORD15

Dietary Supplementation with Natural Extracts Mixture : Effects on Reproductive Performances, Blood Biochemical and Antioxidant Parameters in Rabbit Does

The present study evaluates the effects of natural extracts on reproductive performance, haematochemical parameters, and antioxidant status of rabbit does. A total of sixty New Zealand White second parity does were divided into three groups: The first group was fed a control diet (CON), the second (T1) and the third groups (T2) were fed the same diet supplemented with prebiotic polysaccharides from brown seaweeds (Laminaria spp.) plus phenolic acid, hydroxycinnamic acids, tannins, and flavonoids from plant extracts (0.3% and 0.6%, respectively). The trial was conducted for two consecutive reproductive cycles (75 days). Reproductive performance was recorded. Blood samples were collected before the first insemination, 10 d after the first kindling, and 10 d after the second one. At the first reproductive cycle, productive parameters were negatively affected (P<0.05) by a high dosage of the dietary supplement (T2 group). At the second reproductive cycle, no differences (P>0.05) between dietary treatments on reproductive and productive performances were observed. Bilirubin was affected by dietary treatment (P<0.001) and decreased in relation to sampling time (P<0.001). The HDL cholesterol decreased by dietary treatment (P<0.01). All the plasma antioxidant markers were positively affected (P<0.001) by dietary supplementation and sampling time. No previous study has reported the effects of brown seaweeds and polyphenols on rabbit does and the present data shows that this natural extract supplement improved the antioxidant status of rabbit does