Development of DNA-Biochip for Identification of Influenza A Virus Subtypes

Developed was the DNA-biochip to identify subtypes of influenza A virus, pathogenic for humans. Microchip was capable of detecting H1, H3, H5-subtypes of hemagglutinin (including H1-subtype of pandemic A/H1N1(2009) influenza virus ) and neuraminidase subtypes N1,N2 of influenza virus. This microchip was successfully tested on the strains of A/H5N1 highly pathogenic avian influenza virus, A/H1N1(2009) pandemic influenza virus, A/H1N1 and A/H3N2 seasonal influenza viruses

ПРОБЛЕМЫ РЕВАСКУЛЯРИЗАЦИИ ПРИ ОСТРОМ ИНФАРКТЕ МИОКАРДА С ПОДЪЕМОМ СЕГМЕНТА ST

SUMMARY. Based on the data of 370 patients with acute myocardial infarction with ST segment elevation, problems affecting the efficiency of revascularization treatment by PCI have been analyzed. It has been considered difficult to achieve further reduction in the timing of PCI compared to the available. The acceleration dynamics of ST segment on the electrocardiogram during PCI against thrombolysis and limitations of ECG monitoring during reperfusion in assessing myocardial perfusion have been specified. The necessity and appropriateness of PCI in different periods from the onset of MI have been reasoned. The prospects of using perfusion myocardial SPECT in assessing hypoperfusion and prognosis of restoration of myocardial function have been mentioned. РЕЗЮМЕ. На основании данных о 370 больных острым инфарктом миокарда (ИМ) с подъемом сегмента ST проанализированы проблемы, влияющие на эффективность реваскуляризационного лечения методом чрескожного коронарного вмешательства (ЧКВ). Признано трудно выполнимым дальнейшее сокращение сроков проведения ЧКВ по сравнению с имеющимися. Уточнено ускорение динамики сегмента ST на электрокардиограмме (ЭКГ) при ЧКВ по сравнению с тромболизисом. Уточнены ограничения ЭКГ-контроля при проведении реперфузии в оценке миокардиальной перфузии. Обоснована необходимость и целесообразность выполнения ЧКВ в различные сроки от начала ИМ. Отмечены перспективы использования перфузионной однофотонной эмиссионной компьютерной томографии миокарда в оценке степени гипоперфузии и прогнозе восстановления функции миокарда.

К МЕТОДИКЕ ОЦЕНКИ ПЕРФУЗИИ МИОКАРДА ЛЕВОГО ЖЕЛУДОЧКА ПРИ ИНФАРКТЕ МИОКАРДА С ПОМОЩЬЮ ОДНОФОТОННОЙ ЭМИССИОННОЙ КОМПЬЮТЕРНОЙ ТОМОГРАФИИ

SUMMARY. On the basis of single photon emission computed tomography of the myocardium (SPECT) with Technetril 99mTc performed in 81 patients with myocardial infarction (MI), we confirmed the proportion of myocardial segments of the left ventricle (LV) with varying degrees of RP inclusion and, therefore, with varying degrees of perfusion impairment. In 75% of patients, reduction of hypoperfusion area in the remote period after myocardial infarction has been revealed compared to the acute period. The relation between slow ECG evolution with the severe impairment of left ventricular myocardial perfusion has been showed. The relation between the degree of impairment of LV myocardial perfusion (by SPECT) and its contraction (according to the two-dimensional echocardiogram) has been revealed. It is shown that one of the leading factors in the restoration of myocardial perfusion is the time of intracoronary intervention. РЕЗЮМЕ. На основании проведения у 81 больного инфарктом миокарда (ИМ) перфузионной однофотонной эмиссионной компьютерной томографии (ОФЭКТ) миокарда с 99mTc-технетрилом уточнено соотношение сегментов миокарда левого желудочка (ЛЖ) с разной степенью включения радиофармпрепарата и, следовательно, с разной степенью нарушения перфузии. У 75% пациентов установлено сокращение площади гипоперфузии в отдаленный период инфаркта по сравнению с острым. Продемонстрирована связь замедленной ЭКГ-эволюции ИМ с глубокими расстройствами перфузии миокарда ЛЖ. Выявлена взаимосвязь степени нарушения перфузии миокарда ЛЖ (по данным ОФЭКТ) и его сократимости (по данным двухмерной Эхо-КГ). Показано, что одним из ведущих факторов восстановления перфузии миокарда является время выполнения внутрико- ронарного вмешательства.

“I have no clue what I drunk last night” Using Smartphone technology to compare in-vivo and retrospective self-reports of alcohol consumption.

This research compared real-time measurements of alcohol consumption with retrospective accounts of alcohol consumption to examine possible discrepancies between, and contextual influences on, the different accounts.Building on previous investigations, a specifically designed Smartphone technology was utilized to measure alcohol consumption and contextual influences in de facto real-time. Real-time data (a total of 10,560 data points relating to type and number of drinks and current social / environmental context) were compared with daily and weekly retrospective accounts of alcohol consumption.Participants reported consuming more alcoholic drinks during real-time assessment than retrospectively. For daily accounts a higher number of drinks consumed in real-time was related to a higher discrepancy between real-time and retrospective accounts. This effect was found across all drink types but was not shaped by social and environmental contexts. Higher in-vivo alcohol consumption appeared to be related to a higher discrepancy in retrospectively reported weekly consumption for alcohol beverage types other than wine. When including contextual factors into the statistical models, being with two or more friends (as opposed to being alone) decreased the discrepancy between real-time and retrospective reports, whilst being in the pub (relative to being at home) was associated with greater discrepancies.Overall, retrospective accounts may underestimate the amount of actual, real-time alcohol consumed. Increased consumption may also exacerbate differences between real-time and retrospective accounts. Nonetheless, this is not a global effect as environmental and social contexts interact with the type of alcohol consumed and the time frame given for reporting (weekly vs. daily retrospective). A degree of caution therefore appears warranted with regards to the use of retrospective self-report methods of recording alcohol consumption. Whilst real-time sampling is unlikely to be completely error free, it may be better able to account for social and environmental influences on self-reported consumption

Caveats of chronic exogenous corticosterone treatments in adolescent rats and effects on anxiety-like and depressive behavior and hypothalamic-pituitary-adrenal (HPA) axis function

<p>Abstract</p> <p>Background</p> <p>Administration of exogenous corticosterone is an effective preclinical model of depression, but its use has involved primarily adult rodents. Using two different procedures of administration drawn from the literature, we explored the possibility of exogenous corticosterone models in adolescence, a time of heightened risk for mood disorders in humans.</p> <p>Methods</p> <p>In experiment 1, rats were injected with 40 mg/kg corticosterone or vehicle from postnatal days 30 to 45 and compared with no injection controls on behavior in the elevated plus maze (EPM) and the forced swim test (FST). Experiment 2 consisted of three treatments administered to rats from postnatal days 30 to 45 or as adults (days 70 to 85): either corticosterone (400 μg/ml) administered in the drinking water along with 2.5% ethanol, 2.5% ethanol or water only. In addition to testing on EPM, blood samples after the FST were obtained to measure plasma corticosterone. Analysis of variance (ANOVA) and alpha level of <it>P </it>< 0.05 were used to determine statistical significance.</p> <p>Results</p> <p>In experiment 1, corticosterone treatment of adolescent rats increased anxiety in the EPM and decreased immobility in the FST compared to no injection control rats. However, vehicle injected rats were similar to corticosterone injected rats, suggesting that adolescent rats may be highly vulnerable to stress of injection. In experiment 2, the intake of treated water, and thus doses delivered, differed for adolescents and adults, but there were no effects of treatment on behavior in the EPM or FST. Rats that had ingested corticosterone had reduced corticosterone release after the FST. Ethanol vehicle also affected corticosterone release compared to those ingesting water only, but differently for adolescents than for adults.</p> <p>Conclusions</p> <p>The results indicate that several challenges must be overcome before the exogenous corticosterone model can be used effectively in adolescents.</p

EEG Biofeedback as a Treatment for Substance Use Disorders: Review, Rating of Efficacy, and Recommendations for Further Research

Electroencephalographic (EEG) biofeedback has been employed in substance use disorder (SUD) over the last three decades. The SUD is a complex series of disorders with frequent comorbidities and EEG abnormalities of several types. EEG biofeedback has been employed in conjunction with other therapies and may be useful in enhancing certain outcomes of therapy. Based on published clinical studies and employing efficacy criteria adapted by the Association for Applied Psychophysiology and Biofeedback and the International Society for Neurofeedback and Research, alpha theta training—either alone for alcoholism or in combination with beta training for stimulant and mixed substance abuse and combined with residential treatment programs, is probably efficacious. Considerations of further research design taking these factors into account are discussed and descriptions of contemporary research are given

PROBLEMS OF REVASCULARIZATION IN ACUTE MYOCARDIAL INFARCTION WITH ST SEGMENT ELEVATION

SUMMARY. Based on the data of 370 patients with acute myocardial infarction with ST segment elevation, problems affecting the efficiency of revascularization treatment by PCI have been analyzed. It has been considered difficult to achieve further reduction in the timing of PCI compared to the available. The acceleration dynamics of ST segment on the electrocardiogram during PCI against thrombolysis and limitations of ECG monitoring during reperfusion in assessing myocardial perfusion have been specified. The necessity and appropriateness of PCI in different periods from the onset of MI have been reasoned. The prospects of using perfusion myocardial SPECT in assessing hypoperfusion and prognosis of restoration of myocardial function have been mentioned