Modulational instability of bright solitary waves in incoherently coupled nonlinear Schr\"odinger equations

We present a detailed analysis of the modulational instability (MI) of ground-state bright solitary solutions of two incoherently coupled nonlinear Schr\"odinger equations. Varying the relative strength of cross-phase and self-phase effects we show existence and origin of four branches of MI of the two-wave solitary solutions. We give a physical interpretation of our results in terms of the group velocity dispersion (GVD) induced polarization dynamics of spatial solitary waves. In particular, we show that in media with normal GVD spatial symmetry breaking changes to polarization symmetry breaking when the relative strength of the cross-phase modulation exceeds a certain threshold value. The analytical and numerical stability analyses are fully supported by an extensive series of numerical simulations of the full model.Comment: Physical Review E, July, 199

The First Case of <I>Yersinia Pestis</I> Subsp. <I>Pestis</I> Isolation in the Territory of Altai Mountain Natural Plague Focus. Communication 1. Microbiological Characteristics, Molecular-Genetic and Mass-Spectrometric Identification of the Isolate

Performed is a complex microbiological, molecular-genetic and mass-spectrometric identification of Yersinia pestis main ssp. strain, which was isolated for the first time in the history of surveillance over the Altai mountain natural plague focus in June, 2012. Determined is its high universal virulence. Plasmid screening, multi-locus VNTR- and mass-spectrometric analyses have revealed the strain to be more closely related to the plague agent variant, circulating in the territory of the natural focus Khuukh-Serkh-Munkh-Khairkhan, Bayan-Ul’giisk aimak, Mongolia

Results of Immunological Screening for Natural-Focal and «Exotic» Infectious Diseases among Certain Population Groups of the Khabarovsk Territory, the Amur Region and the Jewish Autonomous Region

Displayed are the results of examination of immuno-competent local population of the Khabarovsk Territory, the Jewish Autonomous Region, the Amur Region, as well as foreign residents living and temporarily working in the areas, on a wide range of natural-focal bacterial and viral infectious diseases including the causative agents of some “exotic” infections too. Investigations have been carried out with the participation of experts from the specialized anti-epidemic team No. 1 (Irkutsk Research Anti-Plague Institute), who worked in the Amur Region, and a group of laboratory-epidemiological specialists from the team No. 2 - deployed in the Khabarovsk Territory and the Jewish Autonomous Region during the flooding in August-September 2013. The total of 1335 blood sera samples has been tested using serological methods. The findings have revealed the presence of immuno-competent population in the three regions of the Far Eastern Federal district in reference to the agents of natural-focal infectious diseases: tularemia, leptospirosis, yersinioses, hemorrhagic fever with renal syndrome, human granulocytic anaplasmosis, tick-borne borrelioses, tick-borne viral encephalitis, Californian encephalitis serogroup, Sindbis, West Nile and Dengue fevers. Circulation of Batai and Crimean-Congo hemorrhagic fever viruses has not been revealed based on serological assays

Analysis of Epidemiological Situation on Leptospiroses in the Amur-River Region. Operational Experience in the Flood Area in 2013, and Forecasting for 2014

For the first time ever mobile SAET units have performed complex investigation of epidemiological situation on leptospiroses in order to provide sanitary-epidemiological welfare of the population in the Amur-River region devastated by high water in 2013. Analyzed have been contemporary published and archival data on morbidity rate and epizootic activity of the natural leptospirosis foci in the Amur-River territory up to 2013. Summarized is the information concerning monitoring over natural focal infections (in particular leptospiroses) in the flooding area, obtained by specialized antiepidemic teams from Irkutsk Research Anti-Plague Institute of Siberia and Far East deployed in Belogorsk town of the Amur Region and Khabarovsk city in 2013. Epizootiological situation during the high water period in the Jewish Autonomous Region has been defined as an extremely adverse, unlike the Khabarovsk Territory and the Amur Region where epizootic process is less expressed. Therewith aggravation of epidemiological situation on leptospiroses in the Amur-River Region in 2014 is not ruled out

Analysis of Epidemiological Situation on Hemorrhagic Fever with Renal Syndrome in the Khabarovsk Territory and the Jewish Autonomous Region, and Prognosis of Its Development for the Past-Flood Period in 2013-2014

Complex analysis of the data concerning epidemiological situation on HFRS in the Khabarovsk Territory and the Jewish Autonomous Region for the past decade (2003-2012) previous to high water in 2013 and results of operative epidemiological inspection of the flooding area and the adjoining territory where a high index of laboratory evidences of the virus presence in the main carriers was revealed, outlines high probability of the epidemiological condition complications in view of the infection in the post-flooding period in 2013-2014 in the both entities. Therewith on the basis of the unfavorable prognosis for the epidemiological situation development a Plan of organizational, preventive and anti-epidemic measures directed to minimization of risks related to aggravation of epidemiological situation on hemorrhagic fever with renal syndrome has been developed and is being implemented

Идентификация маркеров аденокарциномы желудка на основе биоинформатического поиска и анализа генной экспрессии

Introduction. Searching for specific and sensitive molecular tumor markers is one of the important tasks of modern oncology. These markers can be used for early tumor diagnosis and prognosis as well as for prediction of therapeutic response, estimation of tumor volume or to assess disease recurrence through monitoring. Gene expression data base mining followed by experimental validation of results obtained is one of the promising approaches for searching of that kind.Objective: to identify several membrane proteins which can be used for serum diagnosis of intestinal type of gastric adenocarcinoma.Materials and methods. We used bioinformatic-driven search using Gene Ontology and The Cancer Genome Atlas (TCGA) data to identify mRNA up-regulated in gastric cancer (GC). Then, the expression levels of the mRNAs in 55 pare clinical specimens were investigated using reverse transcription polymerase chain reaction.Results. Comparative analysis of the mRNA levels in normal and tumor tissues using a new bioinformatics algorithm allowed to identify 3 high-copy transcripts (SULF1, PMEPA1 and SPARC), intracellular content of which markedly increased in GC. Expression analysis of these genes in clinical specimens showed significantly higher mRNA levels of PMEPA1 and SPARC in tumor as compared to normal gastric tissue. Interestingly more than twofold increase in expression level of these genes was observed in 75 % of intestinal-type GC. The same results were found only in 25 and 38 % of diffuse-type GC respectively.Conclusions. As a result of original bioinforamtic analysis using TCGA data base two genes (PMEPA1 and SPARC) were shown to be significantly upregulated in intestinal-type gastric adenocarcinoma. The findings show the importance of further investigation to clarify the clinical value of their expression level in stomach tumors as well as their role in carcinogenesis.Введение. Одна из важных задач современной онкологии – поиск ассоциированных с опухолями молекулярных маркеров, которые могут использоваться для диагностики и прогнозирования рака, оценки степени радикальности операции и последующего лечения, а также раннего выявления рецидивов. Одним из продуктивных вариантов подобного поиска является анализ транскриптомных баз данных с применением методов биоинформатики с последующей валидацией полученных результатов на клиническом материале.Цель исследования – поиск мембранных белков, которые могут быть использованы для сывороточной диагностики аденокарциномы желудка интестинального гистологического типа.Материалы и методы. Идентификацию потенциальных маркеров рака желудка (РЖ) проводили с использованием баз данных Gene Ontology и the Cancer Genome Atlas (TCGA). Последующую оценку дифференциальной экспрессии генов выполняли на парных образцах аденокарциномы и нормальной ткани желудка, взятых от 55 пациентов. Экспрессию генов оценивали с помощью полимеразной цепной реакции с обратной транскрипцией в режиме реального времени по методу ΔCq.Результаты. Сравнительный анализ уровней синтеза матричных РНК (мРНК) нормальных и опухолевых тканей с применением нового алгоритма биоинформатического поиска привел к идентификации 3 наиболее высококопийных транскриптов (SULF1, PMEPA1 и SPARC), внутриклеточное содержание которых заметно повышается при РЖ. При анализе уровня мРНК данных генов в клиническом материале наблюдалось более чем двукратное увеличение уровня экспрессии PMEPA1 и SPARC в 75 % образцов РЖ интестинального гистологического типа. В образцах РЖ диффузного гистологического типа этот показатель составил 25 и 38 % соответственно.Выводы. Использование оригинального биоинформатического подхода, основанного на анализе данных TCGA, позволило выявить 2 гена (PMEPA1 и SPARC), преимущественно экспрессирующихся в опухолях желудка интестинального типа. Полученные результаты свидетельствуют об актуальности дальнейшего исследования роли этих генов в патогенезе РЖ и оценки клинической значимости уровня их экспрессии в опухолевой ткани

Infection of an Individual with Plague in the Gorno-Altaisk High-Mountain Natural Focus in 2014. Communication 2. Peculiarities of Laboratory Diagnostics and Molecular-Genetic Characterization of the Isolated Strains

Laboratory diagnostics of plague was carried out in compliance with valid operational guidelines and regulations. But its peculiarity consisted in the performance of diagnostic investigations secondary to antimicrobial therapy with application of preparations characterized by the expressed activity towards gram-negative microorganisms, including the agent of plague (ceftriaxone, ciprolet, and amikacin). The studies revealed that under antibiotic treatment during the early phase of infection the most effective method for the laboratory plague diagnostics was PCR. Based on the results of the assay it was possible to establish not only provisional, but also the final diagnosis in a patient. Obtained was genetic characteristics of the strains isolated from the patient and the marmot, withdrawn at the patient’s place, using techniques of molecular-genetic analysis, in particular PCR, multilocus VNTR, and multilocus and genome-wide sequencing. Thereupon the strains were attributed to antique biovar of the main subspecies of plague agent. In addition, close relation to Y. pestis of the main subspecies isolated in the same focus in 2012 and to the strains from Mongolian Altai and Tuvinian mountain focus was determined based on phylogenetic analysis of the isolates

A Giant Bipolar Shell around the WO star in the Galaxy IC 1613: Structure and Kinematics

Observations of the nebula associated with the WO star in the galaxy IC 1613 are presented. The observations were carried out with a scanning Interferometer Fabry-Perot in H_alpha at the 6m Special Astrophysical Observatory telescope; narrow-band H_alpha and [OIII] images were obtained with the 4-m KPNO telescope. The monochromatic H_alpha image clearly reveals a giant bipolar shell structure outside the bright nebula S3. The sizes of the southeastern and northwestern shells are 112x77 pc and (186-192)x(214-224) pc, respectively. We have studied the object's kinematics for the first time and found evidence for expansion of both shells. The expansion velocities of the southeastern and northwestern shells exceed 50 and 70 km/s, respectively. We revealed a filamentary structure of the shells and several compact features in the S3 core. A scenario is proposed for the formation of the giant bipolar structure by the stellar wind from the central WO star located at the boundary of a dense ``supercavity'' in the galaxy's H I distribution.Comment: 10 pages with 4 PS figure

Выявление Bordetella holmesii среди больных, госпитализированных в стационар с подозрением на коклюш или коклюшеподобные заболевания

Purpose. To reveal and estimate prevalence of B. holmesii among the patients hospitalized with suspicion pertussis and pertussis-like illnesses.Materials and methods. 424 clinical samples received from patients with of pertussis and pertussis-like illnesses in GBUZ IKB № 1 DZM in 2017–2018 are investigated. Identification of fragments of a genome of Bordetella was carried out in PCR-RT with “Amplisens® Bordetella multi-FL”. For identification of fragments of a genome of B. holmesii used PCR-RT with primers of IS481, IS1001 and hIS1001.Results. The research included 424 patients, from them 56,1% of children aged till 1 year, 41,3% of children – are more senior than 1 year and 2,6% of adults. When using test system 60,4% of the samples containing DNA of B. pertussis are revealed; 1,9% of samples – DNA of B. parapertussis; in 34,9% of samples it is received negative and in 2,8% – doubtful results. The research of 424 samples in PCR-RT by means of IS481, IS1001 and hIS1001 primers showed that 61,1% of samples contained DNA of B. pertussis; 0,7% of samples – DNA of B. parapertussis and 3,8% of samples – DNA of B. holmesii. In 143 samples the result was negative. From 16 DNA of B. holmesii – positive samples, 9 samples were negative in test system earlier, in 2 samples – the doubtful result, 1 sample was earlier identified as DNA of B. parapertussis and in 4 samples DNA of B. pertussis and B. holmesii are found.Conclusion. The research demonstrates circulation of B. holmesii in the territory of Russia that is confirmed by identification of positive samples in 3,8% of cases among the sick children and adults hospitalized in a hospital with suspicion of pertussis and pertussis-like illnesses. For increase in efficiency of laboratory confirmation of the clinical diagnosis of pertussis and pertussis-like illnesses the genodiagnostic of a pertussis is recommended to improve taking into account identification DNA of B. holmesii.Цель: выявить и оценить распространенность B. holmesii среди больных, госпитализированных в стационар с подозрением на коклюш и коклюшеподобные заболевания.Материалы и методы. Исследовано 424 пробы клинического материала от больных с подозрением на коклюш и коклюшеподобные заболевания, госпитализированных в Инфекционную клиническую больницу № 1 в 2017– 2018 гг. Выявление фрагментов генома бордетелл осуществляли в ПЦР-РВ с «АмплиСенс® Bordetella multi-FL». Для идентификации фрагментов генома B. holmesii использовали ПЦР-РВ с праймерами IS481, IS1001 и hIS1001. Результаты. В исследование включено 424 пациента, из них 57,6% детей до 1 года, 42,3% детей старше 1 года и 2,6% взрослых. При использовании тестсистемы обнаружено 60,4% образцов, содержащих ДНК B. pertussis; 1,9% образцов – ДНК B. parapertussis; в 34,9% образцов получен отрицательный и в 2,8% – сомнительный результаты. Исследование 424 образцов в ПЦР-РВ с помощью IS481, IS1001 и hIS1001 праймеров показало, что 61,1% образцов содержали ДНК B. pertussis; 0,7% образцов – ДНК В. parapertussis и 3,8% образцов – ДНК B. holmesii. В 143 образцах результат был отрицательным. Из 16 ДНК B. holmesii, 9 образцов ранее были отрицательными, в 2 образцах – сомнительный результат и 1 образец был ранее идентифицирован как ДНК B. parapertussis, в 4 образцах обнаружена ДНК B. pertussis и B. holmesii.Заключение. Исследование свидетельствует о циркуляции B. holmesii на территории России, что под тверждается выявлением положительных образцов в 3,8% случаев среди больных детей и взрослых, госпитализированных в стационар с подозрением на коклюш и коклюшеподобные заболевания. Для повышения эффективности лабораторного подтверждения клинического диагноза коклюша и коклюшеподобных заболеваний рекомендуется совершенствовать генодиагностику коклюшной инфекции с учетом идентификации ДНК B. holmesii