Assessing the genetic diversity and the temporal evolution of plant populations for an optimized conservation: the example of the tuberous-rooted chervil.

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Inter Simple Sequence Repeat Fingerprints for Assess Genetic Diversity of Tunisian Garlic Populations

Garlic ( Allium sativum L.) that is cultivated in Tunisia is heterogeneous and unclassified with no registered local cultivars. At present, the level of genetic diversity in Tunisian garlic is almost unknown. Inter Simple Sequence Repeats (ISSR) genetic markers were therefore used to assess the genetic diversity and its distribution in 31 Tunisian garlic accessions with 4 French classified clones used as control. It was the first time that ISSR markers were used to detect diversity in garlic. Seventeen ISSR primers were screened; seven primers detected 73 polymorphic bands. A high level of polymorphic loci (p) was found in Tunisian populations (54%). Nei’s total genetic diversity coefficient was 0.45 and 0.34 respectively for Tunisian and French garlic. Genetic distances observed between Tunisian accessions, ranged between 38.4 and 78.1%. Factor analysis of distances’ table (AFTD) did not classify accessions on the base of geographical origin or morpho-physiological characters, particularly bolting ability, but confirmed the appurtenance of analyzed accessions to s ativum botanical subspecies. There was sufficient diversity detected to start a national collection of garlic germplasm which is crucial for the conservation of genetic diversity and its valorization.   Keywords: Allium sativum L., ISSR markers, genetic diversity, Tunisian garlic populations

Effect of the Photon's Brownian Doppler Shift on the Weak-Localization Coherent-Backscattering Cone

We report the first observation of the dependence of the coherent-backscattering (CBS) enhanced cone with the frequency of the backscattered photon. The experiment is performed on a diffusing liquid suspension and the Doppler broadening of light is induced by the Brownian motion of the scatterers. Heterodyne detection on a CCD camera is used to measure the complex field (i.e., the hologram) of the light that is backscattered at a given frequency. The analysis of the holograms yield the frequency and the propagation direction of the backscattered photons. We observe that the angular CBS cone becomes more narrow in the tail of the Brownian spectrum. The experimental results are in good agreement with a simple theoretical model

Is there variety × isolate interaction in the polygenic quantitative resistance of carrot to Alternaria dauci ?

Horizontal and polygenic resistance is race-nonspecific and, therefore, more durable, unlike vertical resistance, which is race-specific and unstable. However, this division is perhaps not so obvious since some cultivar × isolate interactions have already been observed for plant species with partial resistance. Carrot is known to be partially resistant to Alternaria dauci, but it is relevant for breeders to study cultivar × isolate interactions in order to develop durable resistant varieties. For this purpose, 12 highly diverse carrot genotypes and one segregating population were inoculated in a tunnel or in a glass house with 11 isolates of A. dauci that also represented a high diversity in terms of geographical origin, aggressiveness and genetic diversity. Disease severity values were assessed three times in the tunnel in a one-year experiment (2002) and twice in the glass house in an experiment over two consecutive years (2011 and 2012). The interaction of isolate with genotype was non-significant in the tunnel, and the same result was obtained in the glasshouse for both years of study except for the first scoring date in 2011, suggesting that the partial resistance of carrot to A. dauci is probably mainly explained by major QTLs that confer resistance to a large number of isolates and, potentially, some minor isolate-specific QTLs as well

Evaluation of different methods for the characterization of carrot resistance to the alternaria leaf blight pathogen (Alternaria dauci) revealed two qualitatively different resistances

Alternaria leaf blight (ALB), caused by Alternaria dauci, is one of the most damaging foliar diseases of carrot worldwide. The aim of this study was to compare different methods for evaluating levels of carrot resistance to ALB. Three techniques were investigated by comparison with a visual disease assessment control: in vivo conidial germination, a bioassay based on a drop-inoculation method, and in planta quantification of fungal biomass by quantitative PCR (Q-PCR). Three carrot cultivars showing different degrees of resistance to A. dauci were used, i.e. a susceptible cultivar (Presto) and two partially resistant genotypes (Texto and Bolero), challenged with an aggressive or a very aggressive isolate of A. dauci. Both partially resistant genotypes produced a higher mean number of germ tubes per conidium (up to 3·42±0·35) than the susceptible one (1·26±0·18). The drop-inoculation results allowed one of the partially resistant genotypes (Bolero, log10(S+1) = 1·34±0·13) to be distinguished from the susceptible one (1·90±0·13). By contrast, fungal growth measured by Q-PCR clearly differentiated the two partially resistant genotypes with log10(I) values of 2·77±0·13 compared to the susceptible cultivar (3·65±0·13) at 15 days post-inoculation. This result was strongly correlated (r2 = 0·91) with the disease severity index scored at the same date. Data obtained with the different assessment methods strongly suggest that the Texto and Bolero genotypes have different genetic resistance sources

Is quantitative resistance qualitative? An example with two Alternaria Leaf Blight resistant carrot genotypes and four resistance assessment techniques

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Is host range of carrot restricted to Alternaria dauci?

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Évolution temporelle de la diversité génétique de Chaerophyllum bulbosum : conséquences sur la gestion des ressources génétiques

Afin d’accroître le pool génétique nécessaire à l’amélioration variétale du cerfeuil tubéreux, apiacée alimentaire en pleine expansion, des prospections de populations sauvages sur deux campagnes successives ont été réalisées dans les bassins du Rhin et de la Weser, en Allemagne. Ces populations, principalement ripariennes, sont susceptibles d’être façonnées par les évolutions des réseaux hydrographiques qui caractérisent leur biotope. L’étude moléculaire de la diversité génétique des populations montre une structuration forte entre ces populations sauvages (GST ∼ 32 %), mais ne met pas en évidence d’effet structurant des réseaux hydrographiques sur cette diversité, ni de phénomène d’érosion génétique global. Une discussion sur la stratégie de maintien de la diversité de cette espèce à long terme est proposée