Genome sequence and analysis of methylotrophic yeast Hansenula polymorpha DL1

Ravin NV, Eldarov MA, Kadnikov VV, et al. Genome sequence and analysis of methylotrophic yeast Hansenula polymorpha DL1. BMC Genomics. 2013;14(1): 837.Background: Hansenula polymorpha DL1 is a methylotrophic yeast, widely used in fundamental studies of methanol metabolism, peroxisome biogenesis and function, and also as a microbial cell factory for production of recombinant proteins and metabolic engineering towards the goal of high temperature ethanol production. Results: We have sequenced the 9 Mbp H. polymorpha DL1 genome and performed whole genome analysis for the H. polymorpha transcriptome obtained from both methanol- and glucose-grown cells. RNA-seq analysis revealed the complex and dynamic character of the H. polymorpha transcriptome under the two studied conditions, identified abundant and highly unregulated expression of 40% of the genome in methanol grown cells, and revealed alternative splicing events. We have identified subtelomerically biased protein families in H. polymorpha, clusters of LTR elements at G + C-poor chromosomal loci in the middle of each of the seven H. polymorpha chromosomes, and established the evolutionary position of H. polymorpha DL1 within a separate yeast clade together with the methylotrophic yeast Pichia pastoris and the non-methylotrophic yeast Dekkera bruxellensis. Intergenome comparisons uncovered extensive gene order reshuffling between the three yeast genomes. Phylogenetic analyses enabled us to reveal patterns of evolution of methylotrophy in yeasts and filamentous fungi. Conclusions: Our results open new opportunities for in-depth understanding of many aspects of H. polymorpha life cycle, physiology and metabolism as well as genome evolution in methylotrophic yeasts and may lead to novel improvements toward the application of H. polymorpha DL-1 as a microbial cell factory

Теплопроводность водных растворов хлоридов металлов подгруппы бериллия

The paper presents experimental data on thermal conductivity of BeCl2 and SrCl2 salt aqueous solutions in the temperature range from 20 to 300 °С  and at various electrolyte concentrations  in mass percent. For the first time thermal conductivity of the system Н2О + BeCl2 has been investigated at high temperatures.The experimental results are described with the help of an empirical equation in the form of: λs = λo (1+ Am + Bm3/2 + Cm2),where λs  and λo – thermal conductivity coefficients of solution and water; A, B and C – coefficients depending on electrolyte nature; m – molality in units mol/kg.The formula error is less than  ±1 %.Приведены  экспериментальные данные по теплопроводности водных растворов солей BeCl2 и SrCl2 в области температур от 20 до 300 °С и при различных концентрациях электролита в массовых процентах. Впервые изучена теплопроводность системы Н2О + BeCl2 при высоких температурах.Экспериментальные результаты настоящей работы описываются эмпирическим уравнением в видеλp = λo (1+ Am + Bm3/2 + Cm2),где λp и λo – коэффициенты теплопроводности раствора и воды; А, В и С – коэффициенты, зависящие от природы электролита; m – моляльность в единицах моль/кг.Погрешность формулы меньше ±1 %

Thermal Conductivity of Mixed Aqueous Solutions of NaCl, СаСl₂ and KCl Salts close by Saturation Line

The paper contains experimental data on thermal conductivity of mixed aqueous solutions of salts within the temperature range from 20 to 300 °C and with various electrolyte concentrations in mass percentage. Thermal conductivity of H20-NaCl-CaCl2-KCl system at high temperatures has been studied for the first time. The experimental results of the present investigations have been generalized within the relative coordinates, namely: λ/λ0 and T/T0, (Т0 = 293,15 К; λ0 - thermal conductivity of a solution at T0 temperature). An empirical equation of thermal conductivity in the parabolic form has been obtained

Теплопроводность смешанных водных растворов солей NaCl, СаСl2 и КСl вблизи линии насыщения

The paper contains experimental data on thermal conductivity of mixed aqueous solutions of salts within the temperature range from 20 to 300 °C and with various electrolyte concentrations in mass percentage. Thermal conductivity of H20-NaCl-CaCl2-KCl system at high temperatures has been studied for the first time. The experimental results of the present investigations have been generalized within the relative coordinates, namely: λ/λ0 and T/T0, (Т0 = 293,15 К; λ0 - thermal conductivity of a solution at T0 temperature). An empirical equation of thermal conductivity in the parabolic form has been obtained.Приведены экспериментальные данные по теплопроводности смешанных водных растворов солей в интервале температур от 20 до 300 °С и при различных концентрациях электролита в массовых процентах. Впервые изучена теплопроводность системы H20-NaCl-CaCl2-KCl при высоких температурах. Обобщены экспериментальные результаты настоящей работы в относительных координатах λ/λ0 и T/T0, (Т0 = 293,15 К; λ0 - теплопроводность раствора при температуре Т0). Получено эмпирическое уравнение теплопроводности в параболическом виде

Method for Forecasting Transport Properties of Working Substances used in Industrial Thermal Power System

On the basis of the analysis of the literary data on heat conductivity of water solutions of salts at high temperatures, pressure and concentration the following generalizing formula is received

Aqueous Solution Thermal Conductivity of Beryllium-Subgroup Metal Chlorides

The paper presents experimental data on thermal conductivity of BeCl2 and SrCl2 salt aqueous solutions in the temperature range from 20 to 300 °С  and at various electrolyte concentrations  in mass percent. For the first time thermal conductivity of the system Н2О + BeCl2 has been investigated at high temperatures.The experimental results are described with the help of an empirical equation in the form of: λs = λo (1+ Am + Bm3/2 + Cm2),where λs  and λo – thermal conductivity coefficients of solution and water; A, B and C – coefficients depending on electrolyte nature; m – molality in units mol/kg.The formula error is less than  ±1 %

Метод прогнозирования транспортных свойств рабочих веществ, используемых в промышленной теплоэнергетике

On the basis of the analysis of the literary data on heat conductivity of water solutions of salts at high temperatures, pressure and concentration the following generalizing formula is received.На основе анализа литературных данных о теплопроводности водных растворов солей при высоких температурах, давлениях и концентрациях получена обобщающая формула, по которой рассчитаны коэффициенты теплопроводности для системы H20-NaCl-CaCl2-KCl в области температур (303-573) К и давления (5-50) МПа.