PHARMACEUTICAL STANDARDIZATION OF VATARI GUGGULU

Rasa Shastra is the pharmaceutical branch of Ayurveda. As like any other medical system, success of Ayurvedic treatment also depends upon quality of medicine prescribed to the patient. The integral part of Rasa Shastra lies in the successful pharmaceutical process. Rasaoushadis are the potent Ayurvedic preparations mainly containing metals and minerals. These Oushadis possess wide range of therapeutic efficacy and are considered superior because of their qualities like small dose, quick action, palatability and longer shelf life. Vatari Guggulu is an important Rasa oushadi described in Bhaishajya Ratnavali - Amavata Adhikarana indicated in Gridhrasi. Vatari Guggulu contains Suddha Gandhaka (Sulphur), Suddha Guggulu (Commiphora mukul), Haritaki (Terminalia chebula Retz), Vibhitaki (Terminalia bellirica Roxb), Amlaki (Emblica officinalis Gaertn), Eranda taila (Ricinus communis Linn). The pharmaceutical procedures adopted in this study are Shodhana, Churna Nirmana and preparation of Vati of Vatari Guggulu. The specific pharmaceutical blend of these contents can result in a more effective formulation. Till now, no research work has been carried out to standardize this formulation. Therefore the present study has been planned to standardize the method of preparation of Vatari Guggulu according to the method explained in the classical literature

Clinical evaluation of shankhapani rasa in the management of kashtartava (dysmenorrhoea)

Menstrual pain which is severe enough to limit normal activities is termed as Kashtartava (dysmenorrhoea). Dysmenorrhoea is the most common gynecological problem faced by women due to abnormal anatomical and functional aspect of uterus, pschycosomatic factors, release of prostaglandins, pelvic congestion etc. Shankhapani Rasa which is a Herbo-Mineral formulation posses all the contents having vatahara and gulmahara properties.The clinal Study was carried out to evaluate the efficacy of Shankhapani Rasa in patients suffering from Kashtartava by selecting 30 patients who attended the O.P.D. and I.P.D. of S.V.Ayurvedic Hospital Tirupathi during the period 2011-2012. Patients were given Shankhapani Rasa in a dose of 125mg capsules twice in a day for 1 month randomly and effect was evaluated on pre-test and post-test design.Statistically significant (p<0.01) results were seen in Subjective symptoms like pain during menstruation and duration of pain giving a conclusion that Shankhapani Rasa is effective in the management of Kashtartava

Synthesis and evaluation of anti-oxidant and cytotoxic activities of novel 10-undecenoic acid methyl ester based lipoconjugates of phenolic acids

The synthesis of five novel methyl 10-undecenoate-based lipoconjugates of phenolic acids from undecenoic acid was carried out. Undecenoic acid was methylated to methyl 10-undecenoate which was subjected to a thiol–ene reaction with cysteamine hydrochloride. Further amidation of the amine was carried out with different phenolic acids such as caffeic, ferulic, sinapic, coumaric and cinnamic acid. All synthesized compounds were fully characterized and their structures were confirmed by spectral data. The anti-oxidant activity of the synthesized lipoconjugates of phenolic acids was studied by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and also by the inhibition of linoleic acid oxidation in micellar medium by differential scanning calorimetry (DSC). The prepared compounds were also screened for their cytotoxic activity against five cell lines. It was observed that the lipoconjugates of caffeic acid, sinapic acid, ferulic acid, and coumaric acid displayed anticancer and anti-oxidant properties. The anticancer properties of these derivatives have been assessed by their IC50 inhibitory values in the proliferation of MDA-MB231, SKOV3, MCF7, DU 145 and HepG2 cancer cell lines