Cancer immunology, bioinformatics and chemokine evidence link vaccines contaminated with animal proteins to autoimmune disease: A detailed look at Crohn’s disease and Vitiligo

© 2018, Pharmainfo Publications. All rights reserved. Cancer research has demonstrated that immunization with homologous xenogeneic proteins (such as vaccines contaminated with animal proteins that resemble human proteins) results in autoimmunity. Bioinformatics analysis demonstrates that animal proteins have occasional amino acid differences compared to equivalent human proteins. For this purpose we used Uniprot and BLASTP. We found homology to human GP2 (Bos taurus 77%, Sus scrofa 76%, Cavia porcellus 72% Gallus gallus 43%), homology to human tyrosinase (Bos taurus 87%, Sus scrofa 90%, Cavia porcellus 85%, Gallus gallus 73%), homology to human GP100 (Bos taurus 77%, Sus scrofa 81%, Cavia porcellus 77%, Gallus gallus 42%) and highlight the occasional amino acid differences. Mutated human protein epitopes can be identical to animal protein derived epitopes. Low affinity self reactive T cells suited for detection of mutated human epitopes will be activated by animal derived epitopes. CD8+ T cells involved in numerous autoimmune disorders express the CCR4 skin homing receptor. This is evidence that the site of priming was the skin. This is consistent with subcutaneous or intramuscular injection of animal protein contaminated vaccines. The above findings add to the growing evidence of vaccines inducing autoimmune diseases. Autoantibody and autoreactive T cell levels can vary from person to person. Not everyone will develop overt disease. For every case of diagnosed autoimmune disease, there are numerous subclinical cases. These subclinical diseases could shave decades off your life. So “rare” diagnosed vaccine adverse events are the tip of the iceberg

Autism pathogenesis: Piecing it all together, from end to beginning …

© 2018, Pharmainfo Publications. All rights reserved. Increased extra-axial cerebrospinal fluid (EA-CSF) have been observed in imaging studies of infant brains, who go on to develop autism. Folate deficiency can cause defects in neural development that can affect CSF production and drainage. Folate receptor alpha antibodies (FRAA) are observed in 75% of autism patients. Maternal FRAA have also been observed in the case of neural tube defects. Folate deficiency can cause aluminum accumulation in the brain. Autistic brains have been shown to accumulate aluminum. FRAA in the child or mother can therefore explain all the observations. Further, autism patients have a higher genetic risk for cancer but have lower cancer rates. Many cancer cells express folate receptor alpha to transport folate required for rapid growth. Once again FRAA in autism can thus explain lower rates of cancer occurrence as FRAA block FRA expressed on cancer cells, affecting folate transport. A majority of FRAA are of the IgG4 subclass and bind with higher affinity to the bovine folate receptor than the human folate receptor. The human and bovine FR have 90% protein sequence homology. From allergies and parasite infections we know that IgG4 is the second stage of the immune response. The first stage is IgE against FRA. The US Institute of Medicine concluded that antigens in vaccines do cause IgE mediated sensitization. Many vaccines contain cow’s milk proteins, one of which is the bovine folate receptor protein. Bovine casein and casamino acids used as growth media for vaccine manufacture are derived from cow’s milk. The solution for vaccine-induced IgE against FRA, is to immediately remove all non-target proteins from all vaccines by using processes such as affinity chromatography

Effects of Hot Isostatic Pressing on the Properties of Laser-Powder Bed Fusion Fabricated Water Atomized 25Cr7Ni Stainless Steel

25Cr7Ni stainless steel (super duplex stainless steels) exhibits a duplex microstructure of ferrite and austenite, resulting in an excellent combination of high strength and corrosion resistance. However, Laser-Powder Bed Fusion fabrication of a water-atomized 25Cr7Ni stainless steel of novel chemical composition resulted in a purely ferritic microstructure and over 5% porosity. The current study investigated the effects of two hot isostatic pressing parameters on the physical, mechanical, and corrosion properties as well as microstructures of water-atomized 25Cr7Ni stainless steel of novel composition fabricated by L-PBF for the first time in the literature. The corrosion behaviour was studied using linear sweep voltammetry in a 3.5% NaCl solution. The Hot Isostatic Pressing-treated sample achieved over 98% densification with a corresponding reduction in porosity to less than 0.1% and about 3 similar to 4% in annihilation of dislocation density. A duplex microstructure of ferrite 60% and austenite 40%was observed in the X-Ray Diffraction and etched metallography of the HIP-treated samples from a purely ferritic microstructure prior to the HIP treatment. With the evolution of austenite phase, the HIP-treated samples recorded a decrease in Ultimate Tensile Strength, yield strength, and hardness in comparison with as-printed samples. The variation in the morphology of the evolved austenite grains in the HIP-treated samples was observed to have a significant effect on the elongation. With a reduction in porosity and the evolution of the austenite phase, the HIP-treated samples showed a higher corrosion resistance in comparison with the as-printed samples

Covid-19 disease, women’s predominant non-heparin vaccine-induced thrombotic thrombocytopenia and kounis syndrome: A passepartout cytokine storm interplay

Coronavirus disease 2019 (COVID-19) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) constitute one of the deadliest pandemics in modern history demonstrating cardiovascular, gastrointestinal, hematologic, mucocutaneous, respiratory, neurological, renal and testicular manifestations and further complications. COVID-19-induced excessive immune response accompanied with uncontrolled release of cytokines culminating in cytokine storm seem to be the common pathogenetic mechanism of these complications. The aim of this narrative review is to elucidate the relation between anaphylaxis associated with profound hypotension or hypoxemia with pro-inflammatory cytokine release. COVID-19 relation with Kounis syndrome and post-COVID-19 vaccination correlation with heparin-induced thrombocytopenia with thrombosis (HITT), especially serious cerebral venous sinus thrombosis, were also reviewed. Methods: A current literature search in PubMed, Embase and Google databases was performed to reveal the pathophysiology, prevalence, clinical manifestation, correlation and treatment of COVID-19, anaphylaxis with profuse hypotension, Kounis acute coronary syndrome and thrombotic events post vaccination. Results: The same key immunological pathophysiology mechanisms and cells seem to underlie COVID-19 cardiovascular complications and the anaphylaxis-associated Kounis syndrome. The myocardial injury in patients with COVID-19 has been attributed to coronary spasm, plaque rupture and microthrombi formation, hypoxic injury or cytokine storm disposing the same pathophysiology with the three clinical variants of Kounis syndrome. COVID-19-interrelated vaccine excipients as polysorbate, polyethelene glycol (PEG) and trometamol constitute potential allergenic substances. Conclusion: Better acknowledgement of the pathophysiological mechanisms, clinical similarities, multiorgan complications of COVID-19 or other viral infections as dengue and human immunodeficiency viruses along with the action of inflammatory cells inducing the Kounis syndrome could identify better immunological approaches for prevention, treatment of the COVID-19 pandemic as well as post-COVID-19 vaccine adverse reactions

Role of NMDA receptor autoimmunity induced by food protein containing vaccines, in the etiology of autism, type 1 diabetes, neuropsychiatric and neurodegenerative disorders

© 2019, Advanced Scientific Research. All rights reserved. Vaccines contain numerous animal and plant proteins (soy, peanut, sesame, maize, wheat, etc.). Vaccine excipients are derived from plant or animal sources. The mechanism of animal protein induced autoimmunity was previously described. Following a report associating maternal gluten intake to type 1 diabetes in the offspring, plant proteins were investigated. The Pandemrix vaccine induced narcolepsy due to molecular mimicry between a H1N1 nucleoprotein peptide in the vaccine and the human hypocretin receptor 2. The BLASTP match score for this peptide was used as a baseline. BLASTP showed strong sequence alignment between gliadin, a wheat protein, and the human ionotropic N-methyl-D-aspartate receptor (NMDAR). Analyzing further, strong sequence alignment was found between soy, peanut, sesame, maize, wheat and human glutamate receptors (GR), both ionotropic and metabotropic. There are reports of boosted wheat allergy and de novo synthesis of NMDAR antibodies following immunization. Once immunized with plant derived antigens, antibody levels will be increased by dietary exposure to these antigens. GR are expressed in the brain, heart, pancreas and the T cells of the immune system. Vaccine induced GR antibodies (GRA) disrupt or destroy GR thus precipitating numerous disorders. This explains the epidemic of food intolerances and food associated immune mediated disorders.Intestinal barrier disruption has been proposed as a cause for food associated autoimmune disorders. However, intestinal barrier disruption may itself be the result of GRA. GRA also disrupt the blood-brain barrier. This allows other anti-brain antibodies access to their targets. Vaccine-induced GRA can therefore explain a wide variety of disorders including autism, type 1 diabetes, attention deficit hyperactivity, epilepsy, schizophrenia, autoimmune encephalitis, Huntington’s, Parkinson’s, dementia, cancer and allergies.The ultimate solution is to immediately remove all non-target proteins from all vaccines

Role of NMDA receptor autoimmunity induced by food protein containing vaccines, in the etiology of autism, type 1 diabetes, neuropsychiatric and neurodegenerative disorders

© 2019, Advanced Scientific Research. All rights reserved. Vaccines contain numerous animal and plant proteins (soy, peanut, sesame, maize, wheat, etc.). Vaccine excipients are derived from plant or animal sources. The mechanism of animal protein induced autoimmunity was previously described. Following a report associating maternal gluten intake to type 1 diabetes in the offspring, plant proteins were investigated. The Pandemrix vaccine induced narcolepsy due to molecular mimicry between a H1N1 nucleoprotein peptide in the vaccine and the human hypocretin receptor 2. The BLASTP match score for this peptide was used as a baseline. BLASTP showed strong sequence alignment between gliadin, a wheat protein, and the human ionotropic N-methyl-D-aspartate receptor (NMDAR). Analyzing further, strong sequence alignment was found between soy, peanut, sesame, maize, wheat and human glutamate receptors (GR), both ionotropic and metabotropic. There are reports of boosted wheat allergy and de novo synthesis of NMDAR antibodies following immunization. Once immunized with plant derived antigens, antibody levels will be increased by dietary exposure to these antigens. GR are expressed in the brain, heart, pancreas and the T cells of the immune system. Vaccine induced GR antibodies (GRA) disrupt or destroy GR thus precipitating numerous disorders. This explains the epidemic of food intolerances and food associated immune mediated disorders.Intestinal barrier disruption has been proposed as a cause for food associated autoimmune disorders. However, intestinal barrier disruption may itself be the result of GRA. GRA also disrupt the blood-brain barrier. This allows other anti-brain antibodies access to their targets. Vaccine-induced GRA can therefore explain a wide variety of disorders including autism, type 1 diabetes, attention deficit hyperactivity, epilepsy, schizophrenia, autoimmune encephalitis, Huntington’s, Parkinson’s, dementia, cancer and allergies.The ultimate solution is to immediately remove all non-target proteins from all vaccines

Cancer immunology, bioinformatics and chemokine evidence link vaccines contaminated with animal proteins to autoimmune disease: A detailed look at Crohn’s disease and Vitiligo

© 2018, Pharmainfo Publications. All rights reserved. Cancer research has demonstrated that immunization with homologous xenogeneic proteins (such as vaccines contaminated with animal proteins that resemble human proteins) results in autoimmunity. Bioinformatics analysis demonstrates that animal proteins have occasional amino acid differences compared to equivalent human proteins. For this purpose we used Uniprot and BLASTP. We found homology to human GP2 (Bos taurus 77%, Sus scrofa 76%, Cavia porcellus 72% Gallus gallus 43%), homology to human tyrosinase (Bos taurus 87%, Sus scrofa 90%, Cavia porcellus 85%, Gallus gallus 73%), homology to human GP100 (Bos taurus 77%, Sus scrofa 81%, Cavia porcellus 77%, Gallus gallus 42%) and highlight the occasional amino acid differences. Mutated human protein epitopes can be identical to animal protein derived epitopes. Low affinity self reactive T cells suited for detection of mutated human epitopes will be activated by animal derived epitopes. CD8+ T cells involved in numerous autoimmune disorders express the CCR4 skin homing receptor. This is evidence that the site of priming was the skin. This is consistent with subcutaneous or intramuscular injection of animal protein contaminated vaccines. The above findings add to the growing evidence of vaccines inducing autoimmune diseases. Autoantibody and autoreactive T cell levels can vary from person to person. Not everyone will develop overt disease. For every case of diagnosed autoimmune disease, there are numerous subclinical cases. These subclinical diseases could shave decades off your life. So “rare” diagnosed vaccine adverse events are the tip of the iceberg

Autism pathogenesis: Piecing it all together, from end to beginning …

© 2018, Pharmainfo Publications. All rights reserved. Increased extra-axial cerebrospinal fluid (EA-CSF) have been observed in imaging studies of infant brains, who go on to develop autism. Folate deficiency can cause defects in neural development that can affect CSF production and drainage. Folate receptor alpha antibodies (FRAA) are observed in 75% of autism patients. Maternal FRAA have also been observed in the case of neural tube defects. Folate deficiency can cause aluminum accumulation in the brain. Autistic brains have been shown to accumulate aluminum. FRAA in the child or mother can therefore explain all the observations. Further, autism patients have a higher genetic risk for cancer but have lower cancer rates. Many cancer cells express folate receptor alpha to transport folate required for rapid growth. Once again FRAA in autism can thus explain lower rates of cancer occurrence as FRAA block FRA expressed on cancer cells, affecting folate transport. A majority of FRAA are of the IgG4 subclass and bind with higher affinity to the bovine folate receptor than the human folate receptor. The human and bovine FR have 90% protein sequence homology. From allergies and parasite infections we know that IgG4 is the second stage of the immune response. The first stage is IgE against FRA. The US Institute of Medicine concluded that antigens in vaccines do cause IgE mediated sensitization. Many vaccines contain cow’s milk proteins, one of which is the bovine folate receptor protein. Bovine casein and casamino acids used as growth media for vaccine manufacture are derived from cow’s milk. The solution for vaccine-induced IgE against FRA, is to immediately remove all non-target proteins from all vaccines by using processes such as affinity chromatography