Degradación de la Clorofila Presente en (Spirogyra spp.) como Indicator Socio Ambiental de la Presencia de Plaguicidas organofosforados

En la presente investigación se estudió la alteración de la clorofila presente en Spirogyra spp. como indicador de la presencia de tres plaguicidas organofosforados: Curacrón, Malatión y Acefato, a través de ensayos realizados a diferentes concentraciones de los plaguicidas durante un lapso de 72 horas de exposición. Transcurrida la etapa experimental se pudo observar la pérdida de la coloración verde de las algas como resultado de la alteración de su pigmentación natural. El análisis de varianza realizado a los resultados de la medición de la absorbancia de las muestras demuestra una disminución de la concentración de clorofila, en comparación con las muestras testigo de los ensayos. La sensibilidad de las algas al ser expuestas a los plaguicidas organofosforados es una manifestación del alto potencial bioindicador de Spirogyra spp. Este estudio brinda a los agricultores de la comunidad de San Antonio la posibilidad de detectar de manera temprana la presencia de plaguicidas en sus fuentes de agua y abre las puertas a la investigación para posteriores aplicaciones. The alteration of the chlorophyll present in Spirogyra spp. was studied as an indicator of the presence of three organophosphorus pesticides, Curacron, Malathion and Acephate, with tests carried out at different concentrations of the pesticides during a period of 72 hours of exposure. After the experimental stage, it was possible to observe the loss of the green coloration of the algae as a result of the alteration of their natural pigmentation. The analysis of variance based on the results of the measurement of the absorbance of the samples demonstrated a decrease in the concentration of chlorophyll compared to the control samples from the trials. The sensitivity of algae to being exposed to organophosphorus pesticides is a manifestation of the high bioindicator potential of Spirogyra spp., which provides farmers in the community of San Antonio the possibility of early detection of the presence of pesticides in their sources of water, and it also opens the doors to research for subsequent applications

Impacto de las Abejas (Apis mellifera L.) Como Agentes Polinizadores en el Rendimiento del Cultivo de Zucchini (Cucurbita pepo L.) en el Canton Riobamba, Provincia de Chimborazo

The impact of bees (Apis mellifera L.) as pollinators in the yield of the zucchini (Cucurbita pepo L.) crop in the Riobamba canton, Chimborazo province, was evaluated using a Randomized Complete Block Design (RCBD). This was done using three treatments and three repetitions. The treatments evaluated were: the zucchini cultivation inside the micro tunnel covered with entomological mesh with the presence of bees, the zucchini cultivation inside the micro tunnel covered with anti aphids mesh without the presence of bees, and the open field zucchini cultivation. The evaluated parameters were number of days to the appearance of the fruits, percentage of fertilized and unfertilized flowers, number of fruits per plant, size of fruits, diameter of fruits, weight of fruit, and yield in kg / ha per treatment. The best results achieved in most parametersthat were evaluated such as the percentage of flowering, number of fruits per plant, size of fruit in cm, diameter of fruit in cm, weight of the fruit in grams, yield in kg/ha by treatment, and economic analysis were obtained with the presence of bees (Apis mellifera L.) as pollinating agents in the tunnel. This is in addition to meshes during the flowering stage of the zucchini crop, with an average yield of 14.9 tn/ha.Se evaluó el impacto de las abejas (Apis mellifera L.) como agentes polinizadores en el rendimiento del cultivo de zucchini (Cucurbita pepo L.) en el cantòn Riobamba, provincia de Chimborazo. Para llevar a cabo esta evaluación se utilizó un diseño de bloques completos a azar (DBCA), con tres tratamientos y tres repeticiones. Los tratamientos evaluados fueron: El cultivo de zucchini dentro del micro túnel cubierto con malla entomológica con presencia de abejas, el cultivo de zucchini dentro del micro túnel cubierto con malla antiáfidossin presencia de abejas y el cultivo de zucchini a campo abierto. Los parámetros evaluados fueron número de días transcurridos después de la aparición de los frutos, porcentaje de flores fecundadas y no fecundadas, número de frutos por planta, tamaño de frutos, diámetro de frutos, peso del fruto y rendimiento en kg/ha por tratamiento. Los mejores resultados alcanzados en la mayoría de los parámetros evaluados como el porcentaje de floración, número de frutos por planta, tamaño de fruto en (cm), diámetro de fruto en (cm), peso del fruto en gramos, rendimiento en kg/ha por tratamiento y análisis económico, se obtuvieron con la presencia de las abejas (Apis mellifera L.) como agentes polinizadores en el túnel con mallas durante la etapa de floración del cultivo de zucchini, con un rendimiento en promedio de 14.9 tn/ha

Pathogenicity of Entomopathogenic Fungi on the Weevil of the Andes (Premnotrypes vorax Hustache) of the Potato (Solanum tuberosum L.) in Chimborazo Province, Ecuador

From thirty native isolates of entomopathogenic fungi, which were obtained from corpses of larvae and adults of Andean weevil (Premnotrypes vorax Hustache); two fungi with entomopathogenic characteristics were selected through laboratory tests, which will be used in a program of integrated management of this pest in the Ecuadorian inter-Andean region. The pathogenicity of the 30 isolates was determined by the mortality that they caused in larvae and adults and it was expressed in percentage; for this, a complete randomized design (CRD) was used. The LC50 and TL50 were determined by Probit analysis. The two selected isolates corresponded to: Metarhizium anisopliae (Metschnikoff 1879) Sorokin 1883 (A13) and  Beauveria bassiana (Bals.-Criv.) Vuill. 1912 (A21), which proved to be the best, because they caused the highest mortality in larvae of the fourth instar and adults of P. vorax. The Probit analysis showed that LC50 for A21 isolate (B. bassiana) in fourth instar larvae was obtained with a concentration of 1,75x109 spores/ml and 1,08x109 spores/ml for adults. Instead for A13 isolate (M. anisopliae), the LC50 was obtained with a concentration of 1,65x109 spores/ml for larvae and 2,42x109 spores/ml for adults. The LT50 for the selected isolates at the indicated concentrations, was 4,84 and 4,32 days for larvae with A13 and A21 isolates respectively, while, for adults, the shortest time was obtained with A21 isolate in 4.36 days, compared with A13 isolate, that delayed 20,70 days. The larval stage of P. vorax was the most susceptible to A13 isolate, on the other hand, larvae and adults were equally susceptible to A21 isolate at the indicated concentrations. According to the results obtained, the two selected isolates can be used as an alternative to the use of conventional agrochemicals, which will contribute to a more ecological production

Evaluación de tres dosis de potasio en la producción de lechuga (Lactuca sativa L. var. Crispa) bajo el sistema hidropónico recirculante NTF bajo invernadero

Three doses of potassium were evaluated in the production of lettuce (Lactuca sativa L. var. Crispa), under the recirculable NFT hydroponic system in the greenhouse of the Department of Horticulture of the ESPOCH. A completely randomized block design was used with three treatments and three replications. The doses evaluated were: low dose (LD) with 224,33 ppm, medium dose (MD) with 470,00 ppm, and high dose (HD) with 716,67 ppm. Evaluated parameters were: potassium content in leaves and root, fresh weights of the aerial part and root, yield per net plot and hectare. The economic analysis was carried out according to the relation benefit/cost. The best results for most of the parameters evaluated were obtained with the low dose (LD), obtaining fresh weights of the aerial part and root of 193,01 and 36,65 g respectively. It has a yield per net plot of 2,04 kilograms and per hectare of 15338,75 kilograms, and the highest cost benefit with 4,63 dollars equivalent to 362,75%. On the other hand, the highest content of potassium in leaves and root up to 40 days after transplant (DAT) was obtained with the medium dose (MD) with 5,13 and 6,00% of total potassium, respectively. In all treatments, maintaining turgor in the plants throughout the cycle is very important. From the agronomic and economic point of view, the use a nutritive solution containing 224,33 ppm of potassium was recommended

Efecto De La Humedad Relativa Y La Temperatura En La Aplicacion De Insecticidas Organofosforados Y Carbamatos E Impacto En La Colinesterasa De Agro Productores De Tomate (Solanum Lycopersicum L.) En La Localidad De “San Luis, Chimborazo, Ecuador

This research aimed to determine the effect of relative humidity and temperature in the application of organophosphorus and carbamate insecticides and impact on the colinesterasa of tomato agro producers (Solanum lycopersicum L.) in the location of" San Luis, Chimborazo, Ecuador.The technique used was spectrophotometry. The cholinesterase test was performed on the one hundred and seventy patients of the five communities exposed between one year and more than ten years, in the handling and application of organophosphorus and carbamate insecticides. The method used was kinetic-spectrophotometric (405 nm at 37 ° C). Through the Kruskal Wallis test and the regression and correlation analysis it was determined the significance and the level of correlation and determination between the variables under study and their effect on cholinesterase levels. Concluding that the applications of the organophosphorus and carbamate insecticides under greenhouse with low relative humidity and high temperature, accompanied by the lack of precautions in the management of the crop, contribute to the decrease of the cholinesterase level of the agro producers