Growth, Biomass and Carbon Sequestration Potential of Different Poplar (Populus deltoides Bartr.) clones in Agroforestry System with Wheat (Triticum aestivum) Varieties in tarai belt of Uttarakhand

For two years, field experiments were carried out at Agro-Forestry Research Center of G.B.P.U.A. & T., Pantnagar, Uttarakhand (29°N Latitude, 79°30’ E longitude and at an altitude of 243.84 msl) during 2013-14 and 2014-15, to study the growth, biomass and carbon sequestration potentials of poplar clones in association with different wheat varieties in a sandy loam soil. The soil of the experimental site contained 1.175% of organic carbon, 259, 20 and 195 kg/ha available nitrogen, phosphorus and potassium, respectively. The soil was almost neutral in reaction (pH 8.2). The study was laid in split plot with poplar tree clones viz., S7C8, G-48, W-39 and Kranti in main plots and wheat varieties viz., DBW-17, PBW-502, UP-2748 and HD-2967 in sub-plots. Poplar trees were planted at spacing of 7.0m x 3.0m.Poplar clones S7C8 in 2013-14 and Kranti in 2014-15 recorded higher values of diameter at breast height (DBH) and tree height than clones W-39 and G-48. Wheat variety DBW-17 exhibited higher values of poplar growth than varieties PBW-502 and UP-2748. In 2013-14 clone S7C8 recorded the highest values of different components of trees biomass. In 2014-15, clone Kranti produced significantly higher stem, roots and total biomass than clones G-48 and W-39. The total carbon sequestered by the agroforestry system during both the years, was the highest with the clone Kranti (57.14 t/ha in 2013-14 and 65.69 t/ha in 2014-15). In 2014-15, the total carbon sequestered by clone Kranti was significantly higher than other clones. The total carbon sequestered by the agroforestry system was the lowest with the clone G-48.Except carbon stock in wheat plant biomass, wheat varieties did not affect the poplar growth, biomass and carbon stock of the agroforestry system significantly. Wheat variety DBW-17 in 2013-14 and HD-2967 in 2014-15 recorded the highest amount of carbon in plant biomass

Molecular replacement methods using a parallel processing machine

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Effect of eclampsia on pregnancy outcome at the tertiary care center

Background: Eclampsia is a common medical and life-threatening emergency condition mainly seen in 5-10% of all pregnancies and that is a major cause of maternal and perinatal morbidity and mortality The aim of the study to find out the fetomaternal outcomes of eclampsia in tertiary care hospital and to analyse the trend of eclampsia and associated epidemiological variables.Methods: This retrospective analytical study was undertaken with 40 clinically diagnosed women with eclampsia in their third trimester of pregnancy in the Department of Obstetrics and Gynaecology, at tertiary care hospital from July 2020 to December 2021. Women who came to the hospital with eclampsia or developed eclampsia during hospital stay were included in our study.Results: In our study, the antepartum eclampsia was in 32 cases (80%), primigravida 27 cases (67.5%), maternal age (21-30 years) 26 cases (65%). Cesarean section was the mode of delivery in 26 cases (65%). NICU admission is required by 20 neonates (50%).Conclusions: Eclampsia is an important cause of maternal and perinatal morbidity and mortality. Providing quality antenatal health care services, increasing awareness of patients about warning symptoms, proper investigations, timely delivery, and proper monitoring in the intrapartum and postpartum period have the potential to improve maternal and perinatal outcomes.

Utility of first trimester ultrasound before 12 weeks of gestation at tertiary care centre in western India

Background: The first trimester begins on the first day of the last menstrual period (LMP) and lasts until the end of 12 weeks of gestation. Transvaginal ultrasound is modality of choice for establishing the presence of an intrauterine pregnancy in the first trimester. The focus of our study is routine early pregnancy ultrasound. The purpose of this study was to diagnose various conditions of pregnancy at an early stage by using ultrasound.Methods: We conducted retrospective data analysis of random 250 pregnant patients who had undergone first-trimester ultrasonography USG) (transvaginal/abdominal) in their first antenatal visit at S.V.P. Hospital, Ahmedabad, Gujarat, India from March 2021 to February 2022. The patient was selected by a simple randomized method. Maternal age, parity, gestational age, and special features regarding maternal gestational history were compared with USG findings. Patients were divided into 13 groups on the basis of ultrasonographic diagnosis.Results: We noted 76.8% of patients had single, viable, intrauterine pregnancies, while 23.2% had complicated pregnancies with uterine anomalies, ovarian cysts, leiomyoma, caesarean scar pregnancy or subchorionic hematomas.Conclusions: Ultrasound measurement of fetus in first trimester is most accurate method to confirm gestational age. It is less expensive and easily available modality. First-trimester ultrasound is useful to define embryonic landmarks in developmental stages with reference to gestational age, early diagnosis of miscarriage, ectopic pregnancy, molar pregnancy, multifetal pregnancy, major fetal malformation. And also, to diagnose pregnancy with leiomyoma, caesarean scar pregnancy, uterine anomaly and pre-eclampsia with the help of uterine artery PI

Cloning and structural elucidation of a brassinosteroids biosynthetic gene (Atdwarf4) and genetic transformation of Indian mustard (Brassica juncea L.)

320-330Phytohormones play critical roles in plant growth and development. Brassinosteroids (BRs) are essential group of phytohormones required for optimum growth of plants and their deficiency causes distinctive dwarf phenotypes in plants. Homeostasis of BRs in plants is maintained by DWARF4 enzyme that mediates multiple 22α-hydroxylation steps in brassinosteroid biosynthesis. Arabidopsis plants over-expressing DWARF4 show increase in inflorescence, number of branches and siliques; thereby increased number of seeds/plant. This suggests that engineering DWARF4 biosynthesis in Brassica plant can be strategized to enhance yield in mustard. In the present study (i) we cloned dwarf4 gene from Arabidopsis using gene specific PCR strategy, (ii) elucidated the three-dimensional structure of DWARF4 protein at molecular level where it revealed presence of four beta sheets and 20 alpha-helices, and (iii) transformed mustard cultivar Pusa Jaikisan with an objective to develop transgenic mustard with enhanced number of siliques. We obtained several putative transgenics with an average transformation efficiency of 3.3%. Molecular characterization with nptII specific primers confirmed presence of transgene in six putative transgenic plants

OBTAINING TEMPERATURE FIELDS AS A FUNCTION OF EFFICIENCY IN TIG WELDING BY NUMERICAL MODELING

ABSTRACT The temperature field serves as the basis for predicting levels of residual stress in a welded joint. The aspects related to the mathematical modeling of complex welding procedures were pondered with the FEM: variations in the physical and mechanical properties of the materials as a function of the temperature, the transience and the speed of the welding process, the material phase transformations, the different mechanisms of heat exchange with the environment (convection and radiation), all them associated with a high level of nonlinearity. The heat source used in this analytical model for heat supply was the double ellipsoid model proposed b

Antigen-Displaying Lipid-Enveloped PLGA Nanoparticles as Delivery Agents for a Plasmodium vivax Malaria Vaccine

The parasite Plasmodium vivax is the most frequent cause of malaria outside of sub-Saharan Africa, but efforts to develop viable vaccines against P. vivax so far have been inadequate. We recently developed pathogen-mimicking polymeric vaccine nanoparticles composed of the FDA-approved biodegradable polymer poly(lactide-co-glycolide) acid (PLGA) “enveloped” by a lipid membrane. In this study, we sought to determine whether this vaccine delivery platform could be applied to enhance the immune response against P. vivax sporozoites. A candidate malaria antigen, VMP001, was conjugated to the lipid membrane of the particles, and an immunostimulatory molecule, monophosphoryl lipid A (MPLA), was incorporated into the lipid membranes, creating pathogen-mimicking nanoparticle vaccines (VMP001-NPs). Vaccination with VMP001-NPs promoted germinal center formation and elicited durable antigen-specific antibodies with significantly higher titers and more balanced Th1/Th2 responses in vivo, compared with vaccines composed of soluble protein mixed with MPLA. Antibodies raised by NP vaccinations also exhibited enhanced avidity and affinity toward the domains within the circumsporozoite protein implicated in protection and were able to agglutinate live P. vivax sporozoites. These results demonstrate that these VMP001-NPs are promising vaccines candidates that may elicit protective immunity against P. vivax sporozoites.United States. Dept. of Defense (contract W911NF-07-D-0004)Ragon Institute of MGH, MIT and Harvar