Differential Expression of Glutathione s-Transferase Enzyme in Different Life Stages of Various Insecticide-Resistant Strains of Anopheles Stephensi: A Malaria Vector

Background & objectives: Interest in insect glutathione s-transferases (GSTs) has primarily focused on their role in insecticide resistance. These play an important role in biotransformation and detoxification of many different xenobiotic and endogenous substances including insecticides. The GST activity among 10 laboratory selected insecticide resistant and susceptible/control strains of Anopheles stephensi was compared using the substrates 1-chloro-2,4-dinitrobenzene (CDNB). The difference in the GST activities of different life stages of diverse insecticide resistant strains was compared and presented.Methods: About 100 larvae, pupae, adult males, adult females and eggs (100 μg in total weight) were collected and used for the experiment. The extracts were prepared from each of the insecticide-resistant strains and control. Protein contents of the enzyme homogenate and GST activities were determined.Results: Deltamethrin and cyfluthrin-resistant strains of An. stephensi showed significantly higher GST activity. Larvae and pupae of DDT-resistant strain showed peak GST activity followed by the propoxur-resistant strain. On contrary, the GST activity was found in reduced quantity in alphamethrin, bifenthrin, carbofuran and chloropyrifos resistant strains. Adults of either sexes showed higher GST activity in mosquito strain resistant to organophosphate group of insecticides namely, temephos and chloropyrifos. Interpretation & conclusion: The GST activity was closely associated with almost all of the insecticides used in the study, strengthening the fact that one of the mechanisms associated with resistance includes an increase of GST activity. This comparative data on GST activity in An. stephensi can be useful database to identify possible underlying mechanisms governing insecticide-resistance by GSTs

Insecticide susceptibility status in three medically important species of mosquitoes, Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus, from Bruhat Bengaluru Mahanagara Palike, Karnataka, India

Background: Development of resistance among mosquito vectors of diseases is of growing concern in many countries of the subtropics, including much of India. The distributions of susceptibility/resistance to insecticides are scarcely recorded and are unknown in many parts of India. This manuscript reports the susceptibility status among strains of Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus collected from different geographical areas of Bruhat Bengaluru Mahanagara Palike (BBMP), Karnataka, to six conventionally used insecticides. Results: An. stephensi, Ae. aegypti and Cx. quinquefasciatus from different geographic areas showed susceptibility towards temephos. However, susceptibility was meagre for most of the other insecticides used in the study. Strong resistance to propoxur was reported in Ullala (RR90 = 467.1) and Bannerghatta (RR90 = 12 265) populations of An. stephensi and Ae. aegypti respectively. RR90 â 12 830 was recorded as higher in the Yelahanka (YLK) population of Cx. quinquefasciatus to neem. Significant differences at LC50 and LC90 concentrations were observed against various insecticides in different mosquito populations. Resistance was observed against some of the larvicides for the diagnostic dose recommended by WHO. Conclusion: The results form baseline data on the susceptibility status of three medically important mosquito vectors of diseases to commonly used insecticides in the field at present emphasise the importance of carrying out more effective and planned vector control measures. © 2012 Society of Chemical Industry

Symmetry Energy in the Equation of State of Asymmetric Nuclear Matte

The symmetry energy is an important quantity in the equation of state of isospin asymmetric nuclear matter. This currently unknown quantity is key to understanding the structure of systems as diverse as the neutron-rich nuclei and neutron stars. At TAMU, we have carried out studies, aimed at understanding the symmetry energy, in a variety of reactions such as, the multifragmentation of $^{40}$Ar, $^{40}$Ca + $^{58}$Fe, $^{58}$Ni and $^{58}$Ni, $^{58}$Fe + $^{58}$Ni, $^{58}$Fe reactions at 25 - 53 AMeV, and deep-inelastic reactions of $^{86}$Kr + $^{124,112}$Sn, $^{64,58}$Ni (25 AMeV), $^{64}$Ni + $^{64,58}$Ni, $^{112,124}$Sn, $^{232}$Th, $^{208}$Pb (25 AMeV) and $^{136}$Xe + $^{64,58}$Ni, $^{112,124}$Sn, $^{232}$Th, $^{197}$Au (20 AMeV). Here we present an overview of some of the results obtained from these studies. The results are analyzed within the framework of statistical and dynamical models, and have important implications for future experiments using beams of neutron-rich nuclei.Comment: 10 pages, 4 figures, talk presented at VI Latin American Symposium on Nuclear Physics and Application

Effect Of Oestradiol On Mouse Vaginal Epithelium

Oestradiol which induces the proliferation of the vaginal epithelium is used in the oestrogen replacement therapy for menopausal and postmenopausal disorders. The exact onset of induced cell proliferation and recovery to the controlled level has not been reported before. Hence this study was done in ovariectomised mice and the period of cellular response to ethinyl estradiol, a semisynthetic ester (0.025mg/kg) was recorded. The vaginal epithelium was studied for histological changes and incidence of mitotic figures. The onset of proliferation started at 4h after exposure and reached its maximum at 32h and declined gradually thereafter to reach the base level at 60h

Effective nucleon mass and the nuclear caloric curve

Assuming a schematic form of the nucleon effective mass as a function of nuclear excitation energy and mass, we provide a simple explanation for understanding the experimentally observed mass dependence of the nuclear caloric curve. It is observed that the excitation energy at which the caloric curve enters into a plateau region, could be sensitive to the nuclear mass evolution of the effective nucleon mass.Comment: 5 pages, 5 figures, Accepted for publication in Phys. Rev. C. Minor changes mad

Evaluation of gamma radiation-induced DNA damage in Aedes aegypti using the comet assay

The study was undertaken to evaluate gamma radiation-induced DNA damage in Aedes aegypti. The comet assay was employed to demonstrate the extent of DNA damage produced in adult male A. aegypti exposed to seven different doses of gamma radiation, ranging from 1 Gy to 50 Gy. DNA damage was measured as the percentage of comet tail DNA. A significant linear increase in DNA damage was observed in all samples; the extent of damage being proportional to the dose of gamma radiation the organism received, except in those treated with 1 Gy. The highest amount of DNA damage was noticed at 1 h postirradiation, which decreased gradually with time, that is, at 3, 6 and 12 h postirradiation. This may indicate repair of the damaged DNA and/or loss of heavily damaged cells as the postirradiation time increased. The comet assay serves as a sensitive and rapid technique to detect gamma radiation-induced DNA damage in A. aegypti. This could be used as a potential biomarker for environmental risk assessment. © 2017, © The Author(s) 2017

Retrospective Analysis of Hanging Cases Between 2016 and 2020 in Urban India

Background: Death by hanging is a vital health hazard worldwide; it is classified as violent mechanical deaths resulting from asphyxia. The manner of death in hanging is suicide in the majority of the cases, and accidental hanging is less common, and homicidal hanging is still less common. The study was aimed towards analyzing sociodemographic patterns, precipitating factors for committing hanging at Vijayanagar Institute of Medical Sciences (VIMS), Ballari, India.Methods: A retrospective study was conducted at the mortuary of VIMS, Ballari, Karnataka, India. From January 01, 2016, to December 31, 2020, 356 alleged hanging cases were brought to the mortuary for postmortem examination, and the cause of death was attributed to hanging. The necessary data were collected with the help of history, inquest reports, meticulous postmortem examination, etc. The results were obtained after tabulating and data analyzed with a cross-sectional study.Results: Of 356 cases of hanging, the majority of the cases were in the age group of 31-40 years (140 patients; i.e., 39.32%). Male preponderance was detected in 235(66%) cases, and most victims have married 199(56%) subjects. Concerning seasonal variation, we noted that the maximum number of suicides by hanging was reported in July to September 141(39.60%). Out of 356 hanging cases, 178(50%) were employed. The predisposing factor was Chronic illness in 136(38.20 %) cases, followed by financial stress and psychological problems in 120(33.70%) and 50(14.04%) cases, respectively. Most of the victims belonged to the Hindu religion, 290(82%) cases. Moreover, 320(90%) of cases had no suicide note.Conclusion: Hanging is challenging to prevent due to numerous concomitant factors, but psychological counseling, economic support, and education can reduce the incidence of hanging

Detection of Rouge Drones based on Radio Frequency Classification

The current classification of RF signals from the drone is achieved by leveraging raw signal information of a specific band. The modulation scheme that was found prevailing in commercial drones is Orthogonal Frequency Division Multiplexing (OFDM). OFDM can demodulated to provide information about a raw drone signal. This extracted data is coupled with a machine learning algorithm that is used to classify the signal. Testing of this research is needed to identify better equipment and an optimized test scenario that captures quality data that can be used to train a machine learning algorithm