Digital system of registering, storage and actualization of municipal legal acts: elaboration and approbation in the territory of Krasnoyarsk krai

Objective to determine the legal status of the electronic information system of accounting storing and updating of municipal normative legal acts to disseminate the experience of development and implementation of such a system in Krasnoyarsk krai. Methods general scientific method of dialectical cognition historicallegal systemstructural comparativelegal formallogical and other particular scientific methods of cognition. Results the authors come to the conclusion that the electronic information system of accounting storing and updating of municipal normative legal acts is a type of official information systems and has special qualitative characteristics. Development and implementation of such systems in the activities of local governments according to the authors is a promising direction to ensure the openness of municipal legal acts and the accessibility of information on the results of municipal rulemaking. To illustrate the authorrsquos approach a functional description of the electronic information system of accounting storing and updating of municipal regulatory legal acts ldquoMunicipal legal acts of the Krasnoyarsk krairdquo is given. Scientific novelty consists in the development of theoretical conceptions about the legal nature of official information systems and applied problems of their use in the activities of local governments. Modern information and communication technologies allow the transition to the real embodiment of direct democracy including the involvement of the population in the direct decisionmaking at the level of local government using EISof municipal legal acts. Given the functions that may be performed by EIS ensuring access of citizens to the system of legal acts their storage and systematization communication between local governments and public authorities provision of official authentic texts of a legislative act organization of discussion on drafts of municipal legal acts formation of open data etc. it seems necessary to legislatively establish a legal regime of EIS as the official legal information system. EIS has significant advantages in comparison with other legal systems including the register maintained by the Ministry of Justice of the Russian Federation on the website ldquoNormative legal acts of the Russian Federationrdquo. Additional features of EIS aimed at the implementation of the provisions of the Federal Law ldquoOn ensuring access to information on the activities of state bodies and local selfgovernmentrdquo may occur when the legislator makes changes to the procedure for the official publication of municipal legal acts provided for in part 2 of Article 47 of the Federal Law ldquoOn general principles of local selfgovernment in the Russian Federationrdquo. Practical significance consist in the possibility of wide implementation of the electronic information system of accounting storing and updating of municipal normative legal acts in the activities of local governments developed by the authorsrsquo team

A Meta-Brokering Framework for Science Gateways

Recently scientific communities produce a growing number of computation-intensive applications, which calls for the interoperation of distributed infrastructures including Clouds, Grids and private clusters. The European SHIWA and ER-flow projects have enabled the combination of heterogeneous scientific workflows, and their execution in a large-scale system consisting of multiple Distributed Computing Infrastructures. One of the resource management challenges of these projects is called parameter study job scheduling. A parameter study job of a workflow generally has a large number of input files to be consumed by independent job instances. In this paper we propose a meta-brokering framework for science gateways to support the execution of such workflows. In order to cope with the high uncertainty and unpredictable load of the utilized distributed infrastructures, we introduce the so called resource priority services. These tools are capable of determining and dynamically updating priorities of the available infrastructures to be selected for job instances. Our evaluations show that this approach implies an efficient distribution of job instances among the available computing resources resulting in shorter makespan for parameter study workflows

Structural basis of adenylyl cyclase 9 activation

Adenylyl cyclase 9 (AC9) is a membrane-bound enzyme that converts ATP into cAMP. The enzyme is weakly activated by forskolin, fully activated by the G protein Gαs subunit and is autoinhibited by the AC9 C-terminus. Although our recent structural studies of the AC9-Gαs complex provided the framework for understanding AC9 autoinhibition, the conformational changes that AC9 undergoes in response to activator binding remains poorly understood. Here, we present the cryo-EM structures of AC9 in several distinct states: (i) AC9 bound to a nucleotide inhibitor MANT-GTP, (ii) bound to an artificial activator (DARPin C4) and MANT-GTP, (iii) bound to DARPin C4 and a nucleotide analogue ATPαS, (iv) bound to Gαs and MANT-GTP. The artificial activator DARPin C4 partially activates AC9 by binding at a site that overlaps with the Gαs binding site. Together with the previously observed occluded and forskolin-bound conformations, structural comparisons of AC9 in the four conformations described here show that secondary structure rearrangements in the region surrounding the forskolin binding site are essential for AC9 activation

Multidrug efflux pumps:structure, function and regulation

Infections arising from multidrug-resistant pathogenic bacteria are spreading rapidly throughout the world and threaten to become untreatable. The origins of resistance are numerous and complex, but one underlying factor is the capacity of bacteria to rapidly export drugs through the intrinsic activity of efflux pumps. In this Review, we describe recent advances that have increased our understanding of the structures and molecular mechanisms of multidrug efflux pumps in bacteria. Clinical and laboratory data indicate that efflux pumps function not only in the drug extrusion process but also in virulence and the adaptive responses that contribute to antimicrobial resistance during infection. The emerging picture of the structure, function and regulation of efflux pumps suggests opportunities for countering their activities

Influence of continuous magnetic field on the performance of molecularly imprinted polymers

A set of polymers was imprinted with (−)-ephedrine using UV initiation, under the influence of a constant external magnetic field with intensities ranging from 0 to 1.55 T. Synthesised materials were characterised by X-ray crystallography, infrared spectroscopy, swelling and surface area. Recognition properties were assessed by the ability to discriminate between (+) and (−)- ephedrine and by Scatchard analyses on chromatographic mode. It was shown that polymer morphology and recognition properties are affected by the magnetic field. This resulted in considerable improvements in the chromatographic resolution of ephedrine enantiomers by materials synthesised under the influence of magnetic field. Apparently the magnetic field improved the ordering of the polymer structure and facilitated the formation of more uniform imprinting