Plant-produced cottontail rabbit papillomavirus L1 protein protects against tumor challenge : A proof-of-concept study

The native cottontail rabbit papillomavirus (CRPV) L1 capsid protein gene was expressed transgenically via Agrobacterium tumefaciens transformation and transiently via a tobacco mosaic virus (TMV) vector in Nicotiana spp. L1 protein was detected in concentrated plant extracts at concentrations up to 1.0 mg/kg in transgenic plants and up to 0.4 mg/kg in TMV-infected plants. The protein did not detectably assemble into viruslike particles; however, immunoelectron microscopy showed presumptive pentamer aggregates, and extracted protein reacted with conformation-specific and neutralizing monoclonal antibodies. Rabbits were injected with concentrated protein extract with Freund's incomplete adjuvant. All sera reacted with baculovirus-produced CRPV L1; however, they did not detectably neutralize infectivity in an in vitro assay. Vaccinated rabbits were, however, protected against wart development on subsequent challenge with live virus. This is the first evidence that a plant-derived papillomavirus vaccine is protective in an animal model and is a proof of concept for human papillomavirus vaccines produced in plants. Copyright © 2006, American Society for Microbiology. All Rights Reserved

Dismorfia muscular Muscle dysmorphia

Preocupações mórbidas com a imagem corporal eram tidas até recentemente como problemas eminentemente femininos. Atualmente estas preocupações também têm sido encontradas no sexo masculino. A dismorfia muscular é um subtipo do transtorno dismórfico corporal que ocorre principalmente em homens que, apesar da grande hipertrofia muscular, consideram-se pequenos e fracos. Além de estar associada a prejuízos sociais, ocupacionais, recreativos e em outras áreas do funcionamento do indivíduo, a dismorfia muscular é também um fator de risco para o abuso de esteróides anabolizantes. Este artigo aborda aspectos epidemiológicos, etiológicos e padrões clínicos da dismorfia muscular, além de tecer comentários sobre estratégias de tratamento para este transtorno.<br>Morbid concern over body image was considered, until recently, a female issue. Nowadays, it has been viewed as a common male disorder. Muscle dysmorphia, a subtype of a body dysmorphic disorder, affects men who, despite having clear muscular hypertroph,y see themselves as frail and small. Besides being associated to major social, leisure and occupational dysfunction, muscle dysmorphia is also a risk factor for the abuse of steroids. This article describes epidemiological, etiological and clinical characteristics of muscle dysmorphia and comments on its treatment strategy

Validity And Reliability Study Of Turkish Version Of “Muscle Dysmorphic Disorder Inventory” And “Bodybuilder Image Grid” Scales

Background: Although bigorexia symptoms are rapidly increasing, it is mostly an under recognized condition in Turkish male bodybuilders. There are no validated screening tools to identify the symptoms. Objective: The purpose of this study is to evaluate the validity of the Turkish version of the MDDI and the BIG towards the diagnosis of bigorexia and to provide health care professionals with early screening tools. Methods: One hundred twenty male bodybuilders, fifty-eight professional bodybuilders and six tytwo recreational bodybuilders, all of whom matched the research criteria, were included in this study. MDDI and BIG forms were filled by the bodybuilders, along with an “Individual Characteristic Information Form”, a “Nutrition-related Information Form”, and a “Bodybuilding-related Information Form”. To evaluate the construct validity, factor analysis was conducted and resulted in a three factor construct. Results: The factor-loading values ranged from 0.542- 0.827. Calculations of Cronbach’s alpha for the MDDI sum (α = 0.657) revealed a good internal consistency. The MDDI, BIG O, and BIG S intra class correlation coefficients (ICC) were found to be 0.840, 0.908, and 0.879, respectively. As a result, MDDI had acceptable reliability and that of BIG O and BIG S was excellent. Discussion: Turkish MDDI, BIG-O and BIG-S forms proved to be valid and reliable scales and were adequate for determining the symptoms of bigo rexia in male bodybuilders. Using these forms, there was a statistically significant relationship between bigorexia and eating disorders, which were significantly positively correlated. Conclusion: Our results support the feasibility of using the MDDI, the BIG-O, and the BIG-S forms to determine symptoms of bigorexia in Turkish population. Further studies are needed to confirm if this result can be generalized to female bodybuilders. © 2019 Bentham Science Publishers.Scopu