Microbial Degradation Of Calcium Oxalate And Its Potential Application

Calcium oxalate is a naturally occurring compound found in various plants and minerals, and its accumulation in the kidney leads to the formation of kidney stones in humans. One of the main causes of kidney stone formation is a decrease in the number of intestinal microbiota which are capable of degrading calcium oxalate. This study focuses on bacterial degradation of calcium oxalate accumulated in humans. Searching for Calcium oxalate degrading strain with high degrading activity has become one of the priorities from the context of research. The study aimed to isolate and characterize microorganisms which are capable of degrading calcium oxalate. Calcium oxalate degrading microorganisms were isolated from faecal samples by using MRS-calcium oxalate medium. Calcium oxalate degrading bacteria were identified on the basis of colour reduction on calcium oxalate medium plate after the incubation period. The morphological and biochemical characterization of isolates were studied. Quantitative determination of degradation was performed by using an indole assay. The activity of isolates was observed by the degradation of kidney stones. Isolates showed better results for degradation of kidney stones after 2 weeks of the incubation period

Procedure for preparing permeabilized hyphae for enzyme assays

Procedure for preparing permeabilized hyphae for enzyme analysis

Separation and characterization of biosurfactant from P.aeruginosa sp1 isolated from oil mill area MIDC, Parbhani (M.S.)

Biosurfactant are an amphiphilic compound that reduces surface and interfacial tensions by accumulating at the interface of immiscible fluids and increase the surface areas of insoluble compounds leading to increased mobility. The extraction of the biosurfactant from the cell free supernatant using the solvent extraction procedure and the quantitative analysis has been discussed. The application of the biosurfactant includes biomedical, cosmetics and bioremediation rhamnolipid biosurfactant produced by Pseudomonas aeruginosa sp showed significant applications in the bioremediation of hydrocarbon in gasoline spilled oil and petroleum oily sludge in this review we discuss the potential roles and applications of biosurfactant, mainly focusing on areas such as food and food related industries, biomedicine and therapeutics

Screening and optimization of biosurfactant producing bacteria from oil mill area MIDC, Parbhani (M.S.)

Biosurfactant are amphiphilic compounds produced on living surfaces, mostly on microbial cell surfaces. They are biodegradable non- toxic and ecofreindly materials. Pseudomonas aeruginosa isolated from oil mill area produced biosurfactant at 370C at pH 7 on YPG medium and mineral salt medium. The biosurfactant production depends on the fermentation conditions, environmental factors and nutrient availability. The extraction of the biosurfactant from the cell free supernatant using the solvent extraction procedure & the qualitative and quantitative analysis has been discussed with appropriates equipment details

Shadow Detection and Removal in Single-Image Using Paired Regions

A shadow appears on an area when the light from a source cannot reach the area due to obstruction by an object. The shadows are sometimes helpful for providing useful information about objects, and sometimes it degrade the quality of images or it may affect the information provide by them. Thus for the correct image interpretation it is important to detect shadow and restore the information. However, shadow causes problems in computer vision applications, such as segmentation, object detection and object counting. That’s why shadow detection and removal is a pre-processing task in many computer vision applications. So we propose a simple method to detect and remove shadows from a single image. The proposed method begins by selecting shadow image and by pre-processing method we focus only on shadow part. In image classification we distinguish between shadow and non shadow pixels. So that we able to label shadow and non shadow regions of the image. Once shadow is detected that detection results are later refined by image matting, and the shadow- free image is recovered by removing shadow region by non shadow region. Examination of a number of examples indicates that this method yields a significant improvement over previous methods

Analytical Review on Raloxifene -An Estrogen Receptor Modulator in Different Pharmaceutical Formulations and Biological Fluids

Raloxifene (RLX) is an oral selective estrogen receptor modulator (SERM). It is showing estrogenic action on bone and anti-estrogenic action on uterus and breast. An extensive literature has been published for analysis of RLX in different pharmaceutical formulations. This review article endeavor to provide the detail account on analytical methods for RLX and also validation details for its readers. It further helps to avoid costly chemicals and time consuming exercises for further investigation of RLX

Performance Comparison of Different Load Balancing Algorithms in Cloud Computing

Cloud computing offers economical, scalable, and instantaneous computing resources to enterprises, allowing them to manage substantial traffic volumes and cater to a multitude of users. But the need for effective load balancing techniques has grown significantly as cloud computing becomes more and more popular. To guarantee the best possible performance, availability, and dependability of apps and services, load balancing is a crucial component of cloud computing. This paper offers a comparative study of different cloud computing technologies and load balancing strategies. We present a performance comparison of software-based load balancing; our analysis compares various service broker policies, such as closest distance, optimized, and reconfigurable, with algorithms such as round robin, throttled, and equally spread. Overall, this paper helps readers understand load balancing mechanisms in cloud computing

Functional transcriptomic analysis of the role of MAB-5/Hox in Q neuroblast migration in Caenorhabditis elegans

Background: Directed cell migration is a fundamental process in normal development and in tumor metastasis. In C. elegans the MAB-5/Hox transcription factor is a determinant of posterior migration of the Q neuroblast descendants. In this work, mab-5 transcriptional targets that control Q descendant migration are identified by comparing RNA-seq profiles in wild type and mab-5 mutant backgrounds. Results: Transcriptome profiling is a widely-used and potent tool to identify genes involved in developmental and pathological processes, and is most informative when RNA can be isolated from individual cell or tissue types. Cell-specific RNA samples can be difficult to obtain from invertebrate model organisms such as Drosophila and C. elegans. Here we test the utility of combining a whole organism RNA-seq approach with mab-5 loss and gain-of-function mutants and functional validation using RNAi to identify genes regulated by MAB-5 to control Q descendant migration. We identified 22 genes whose expression was controlled by mab-5 and that controlled Q descendant migration. Genes regulated by mab-5 were enriched for secreted and transmembrane molecules involved in basement membrane interaction and modification, and some affected Q descendant migration. Conclusions: Our results indicate that a whole-organism RNA-seq approach, when combined with mutant analysis and functional validation, can be a powerful method to identify genes involved in a specific developmental process, in this case Q descendant posterior migration. These genes could act either autonomously in the Q cells, or non-autonomously in other cells that express MAB-5. The identities of the genes regulated by MAB-5 indicate that MAB-5 acts by modifying interactions with the basement membrane, resulting in posterior versus anterior migration

Extraction, purification and analysis of thermal stability of xylose isomerase

Thermostable enzymes are the enzymes which active even at high temperatures, such enzymes are industrially as well as biochemically very important. Xylose isomerase (EC 5.3.1.5) is one such enzyme with suitable commercial applications. It is heat stable and does not require expensive cofactors such as NAD or ATP for activity. The microorganisms producing this enzyme were isolated from hot water spring near ‘Surat’. The organisms were isolated and purified by using different screening methods. The isolated organisms were then subjected to optimum growth conditions for enzyme production. This enzyme was then assayed for its thermal stability at elevated temperatures by using DNSA