109 research outputs found
HGF-Transgenic MSCs Can Improve the Effects of Tissue Self-Repair in a Rabbit Model of Traumatic Osteonecrosis of the Femoral Head
BACKGROUND: Osteonecrosis of the femoral head (ONFH) is generally characterized as an irreversible disease and tends to cause permanent disability. Therefore, understanding the pathogenesis and molecular mechanisms of ONFH and developing effective therapeutic methods is critical for slowing the progress of the disease. METHODOLOGY/PRINCIPAL FINDINGS: In this study, an experimental rabbit model of early stage traumatic ONFH was established, validated, and used for an evaluation of therapy. Computed tomography (CT) and magnetic resonance (MR) imaging confirmed that this model represents clinical Association Research Circulation Osseous (ARCO) phase I or II ONFH, which was also confirmed by the presence of significant tissue damage in osseous tissue and vasculature. Pathological examination detected obvious self-repair of bone tissue up to 2 weeks after trauma, as indicated by revascularization (marked by CD105) and expression of collagen type I (Col I), osteocalcin, and proliferating cell nuclear antigen. Transplantation of hepatocyte growth factor (HGF)-transgenic mesenchymal stem cells (MSCs) 1 week after trauma promoted recovery from ONFH, as evidenced by a reversed pattern of Col I expression compared with animals receiving no therapeutic treatment, as well as increased expression of vascular endothelial growth factor. CONCLUSIONS/SIGNIFICANCE: These results indicate that the transplantation of HGF-transgenic MSCs is a promising method for the treatment for ONFH and suggest that appropriate interference therapy during the tissue self-repair stage contributes to the positive outcomes. This study also provides a model for the further study of the ONFH etiology and therapeutic interventions
Osteonecrosis in inflammatory bowel diseases: a review of the literature
INTRODUCTION: Osteonecrosis (ON) of the femoral head can lead to femoral head
collapse, necessitating total hip replacement. Reports of patients suffering from
both ON and Inflammatory Bowel Diseases (IBD) have prompted us to evaluate the
relationship between ON and IBD, especially Crohn's disease and ulcerative
colitis. METHODS: A review of the data from three new cases, along with data from
all the published cases of patients presenting ON and IBD found through a
systematic search of the Pub Med database. RESULTS: We encountered some
diagnostic problems: The ON diagnosis could not be confirmed in some patients who
did not meet the ON diagnostic criteria. Reviewed data was too weak to assess the
exact incidence of ON in IBD. Corticosteroid therapy, especially in high dose
regimens, is likely the most important etiological factor. No evidence supporting
other physiopathological hypothesis, such as total parenteral nutrition,
osteoporosis, or coagulation disorders, was found. Finally, the multifocal form
of ON appears particularly common in IBD, with some patients presenting multiple
lesions of the hip, shoulder, knee and talus. CONCLUSIONS: ON in IBD, which is
frequently multifocal, appears to be a complication of corticosteroid therapy,
especially when high doses are used. We recommend regular ON checkups for
corticosteroid-treated IBD patients
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