UN APPROCCIO TRASCRIZIONALE, MOLECOLARE E FUNZIONALE PER DEFINIRE L¿ONTOGENIA DEI NEURONI STRIATALI UMANI

The brain is the most complex of biological systems and our knowledge on the developmental mechanisms underlying its formation and organization have been based on studies in rodents. Little is know about the human brain development. To shed light on the telencephalic developmental program in humans we investigated the transcriptional profile that distinguish the developing striatum from the cortex and we define the molecular set that identify the different populations present in the differentiating striatum from 2 to 20 gestational weeks. This thesis represent the first description of the spatio-temporal expression profile that drive the differentiation of striatal precursors towards a medium spiny neurons fate and molecularly define the striatal compartments. New striatal determinants were identified and unexpected differences from mouse striatal development were discovered. Finally we compared the transcriptional profiles of the developing striatum and cortex with striatal and cortical primary neurons differentiated in vitro. We found an enrichment of function-related transcripts in human primary neurons that were confirmed by electrophysiological analysis revealing the presence of (i) Na+, K+ and Ca2+ currents, (ii) GABA, glutamate and dopamine receptors response and (iii) spontaneous synaptic activity

THE ROLE OF PATHOLOGY IN THE DEVELOPMENT AND EVALUATION OF MURINE XENOGRAFT MODELS OF HUMAN CANCER

Animal models are extensively used to understand how cancer develops and spreads and to test the efficacy of novel therapeutic strategies. Preclinical cancer studies carried out on animal models fall into two broad categories, those using tumor cell transplantation and those in which tumors arise or are induced in the host. During the course of my PhD, I dealt with a wide array of different experimental studies based on xenografting of primary human tumor tissuesin immunodeficient mice. The aim of this thesis is to provide a basic panel for a pathological approach to human tumor xenografts in mice, with detailed references to subcutaneous (s.c.) xenograft models, intraperitoneal (i.p.) xenograft models and to promising metastatic models. The goal of my three-year-research is to critically evaluate how each model has been utilized to provide insights into human cancer therapy and to provide an assessment of the strengths and limitations of each model

Cultura do girassol: tecnologia de produção.

Escolha da area e preparo do solo; Correcao da acidez; Adubacao; Epoca de plantio; Plantio; Densidade; Controle de plantas daninhas; Doencas e pragas; Colheita.bitstream/item/60796/1/Documentos-67.pd

Cultura do girassol: tecnologia de produção.

Escolha de area e preparo do solo; Correcao da acidez; Adubacao; Epoca de plantio; Plantio; Densidade; Controle de plantas daninhas; Doencas e pragas; Colheita.bitstream/item/61288/1/Documentos-67-1996.pdf2. ed. rev. aum

Ocorrência da podridão branca de capitulo e haste causada por Sclerotinia sclerotiorum nos cultivos de girassol, em semeadura após a colheita da safra de verão, no estado do Paraná.

bitstream/item/77938/1/CNPSO-PESQ.-AND.-22-99.pd

Análise da capacidade combinatória entre linhagens de girassol.

A capacidade geral (CGC) e a capacidade específica de combinação (CEC), entre duas linhagens macho-estéreis (mãe) e um grupo de sete linhagens S4 restauradoras de fertilidade (pai), foram estimadas para estudar o potencial desses materiais em programas de melhoramento de girassol (Helianthus annuus L.). O método IV de Griffing, adaptado para cruzamentos dialélicos parciais, foi usado na análise das combinações híbridas. Considerando a CGC para rendimento de aquênios e teor de óleo, os progenitores com maior potencial para o melhoramento foram CMS HA 302 (originária de uma população norte-americana) para ser usada como mãe, e as linhagens 89V2345)3382 e 89V2345)3311 (derivadas da população V2000, obtida por seleção sobre a população Issanka, originária da França) como pais nos cruzamentos. Para o rendimento de aquênios, os efeitos gênicos não aditivos foram importantes na determinação das diferenças entre progenitores. Considerando-se os efeitos gênicos aditivos e não-aditivos conjuntamente, as melhores combinações são CMS HA 302 x 89V2396)5333 para rendimento de aquênios e CMS HA 30379NW22 x 89V2345)3382 para teor de óleo e rendimento de óleo

On subreducts of subresiduated lattices and logic

Subresiduated lattices were introduced during the decade of 1970 by Epstein and Horn as an algebraic counterpart of some logics with strong implication previously studied by Lewy and Hacking. These logics are examples of subuintuitionistic logics, i.e., logics in the language of intuitionistic logic that are defined semantically by using Kripke models, in the same way as intuitionistic logic is defined, but without requiring of the models some of the properties required in the intuitionistic case. Also in relation with the study of subintuitionistic logics, Celani and Jansana get these algebras as the elements of a subvariety of that of weak Heyting algebras. Here, we study both the implicative and the implicative-infimum subreducts of subresiduated lattices. Besides, we propose a calculus whose algebraic semantics is given by these classes of algebras. Several expansions of this calculi are also studied together to some interesting properties of them