20 research outputs found
Fallenversuche zur Kennzeichnung des Verhaltens von Schmeissfliegen Gegen�ber Verschiedenen Merkmalen Ihrer Umgebung
Chemical RNA digestion enables robust RNA-binding site mapping at single amino acid resolution
© 2020, The Author(s), under exclusive licence to Springer Nature America, Inc. RNA-binding sites (RBSs) can be identified by liquid chromatography and tandem mass spectrometry analyses of the protein-RNA conjugates created by crosslinking, but RBS mapping remains highly challenging due to the complexity of the formed RNA adducts. Here, we introduce RBS-ID, a method that uses hydrofluoride to fully cleave RNA into mono-nucleosides, thereby minimizing the search space to drastically enhance coverage and to reach single amino acid resolution. Moreover, the simple mono-nucleoside adducts offer a confident and quantitative measure of direct RNA-protein interaction. Using RBS-ID, we profiled similar to 2,000 human RBSs and probedStreptococcus pyogenesCas9 to discover residues important for genome editing11sci