Additional file 1 of Selective gene expression profiling contributes to a better understanding of the molecular pathways underlying the histological changes observed after RHMVL

Additional file 1: List of gene primers

Additional file 2 of Selective gene expression profiling contributes to a better understanding of the molecular pathways underlying the histological changes observed after RHMVL

Additional file 2: List of all normalized gene expression data

Additional file 1: Figure S1. of Short-term treatment with taurolidine is associated with liver injury

Cell survival and LC50 of HepaRG cells after tauroldine treatment for 24 h was assessed by WST-assay. The LC50 for taurolidine was about 125 μg/ml (n = 6 per concentration, error bars = standard deviation). (TIFF 169 kb

Additional file 2: Figure S2. of Proteinase-activated receptor 2 (PAR2) in hepatic stellate cells – evidence for a role in hepatocellular carcinoma growth in vivo

The effect of PAR2-AP on [Ca2+]i mobilisation in LX-2 cells is dose-dependent and can be blocked by the PAR2 antagonist GB 88. A and B) LX-2-wt cells grown on Lab Tek chambered borosilicate cover glass were loaded with fluo-4-AM as outlined in the in Method section. For calcium measurements, an inverted confocal laser scanning microscope LSM 510 was used and fluorescence was monitored at 488 nm. A) Dose-response relationship of PAR2-AP-induced calcium mobilisation. LX-2 cells were stimulated with PAR2-AP for 15 s at indicated concentrations. Data represent the mean ± SD from calcium measurements in 20 individual cells and are representative for 5 independent experiments. B) GB 88 blocks the calcium signal induced by PAR2-AP, but is unable to inhibit the effect of PAR1-AP on [Ca2+]i mobilisation in LX-2 cells. LX-2 cells were preincubated with PAR2 antagonist GB 88 (10 μM) for 1 h and subsequent stimulated with PAR2-AP (10 μM) and PAR1-AP TFLLRN-NH2 (100 μM). The arrows indicate the time of addition of PAR2-AP and PAR1-AP (data are representative for 8 independent experiments). (TIF 19 kb

Runoff extremity in the Upper Lužnice catchment area

Thesis subject is the evaluation of runoff and flood regime of rivers Lužnice and Skřemelice at the closing profiles just before their confluences. The results are compared with findings from the profile Pilař, which were published in the past by other authors. More attention is paid to the evaluation of the hydrological year 2013 and in detail is described the flood in June of the same year. Daily flow data from the years 1971 - 2014 were used for evaluating of runoff conditions. The assessment of the runoff regime in terms of daily, monthly and annual flows were compared with the runoff regime in Pilař gauge station. The source regions with dominating influence on the resulting runoff were discovered. Analysis of the flood regime confirmed that spring floods in Lužnice came mainly from upland and hilly parts of catchment and large summer floods have main source area in the catchment of Lužnice river itself, before the confluence of the Lužnice river and Skřemelic river. When assessing flood in 2013, the main source areas of flood flows that hit Lužnice river basin were founded. The flood extremity was compared in each closing profiles

Flow chart illustrating the consecutive steps that were followed to select patients who met the Mayo Clinic criteria for liver transplantation, but were not treated with neo-adjuvant chemoradiotherapy.

<p>Flow chart illustrating the consecutive steps that were followed to select patients who met the Mayo Clinic criteria for liver transplantation, but were not treated with neo-adjuvant chemoradiotherapy.</p

Clinicopathological variables of patients undergoing liver transplantation for hilar cholangiocarcinoma.

<p>Thirty four patients who underwent an attempt to surgically remove the tumor prior to transplantation and 8 patients with missing variables were excluded.</p

Univariate analysis for five-year survival in patients transplanted for hilar cholangiocarcinoma.

<p>Thirty four patients who underwent an attempt to surgically remove the tumor prior to transplantation and 8 patients with missing variables were excluded.</p

Mayo clinic criteria for inclusion in the transplantation protocol for hilar cholangiocarcinoma[15–17].

<p>Mayo clinic criteria for inclusion in the transplantation protocol for hilar cholangiocarcinoma[<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0156127#pone.0156127.ref015" target="_blank">15</a>–<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0156127#pone.0156127.ref017" target="_blank">17</a>].</p

Additional file 3: of Chemerin in peritoneal sepsis and its associations with glucose metabolism and prognosis: a translational cross-sectional study

Figure S2. Correlation of fasting and intra-operative glucose levels with circulating chemerin in controls. a Correlation of circulating chemerin levels with fasting glucose levels (r = 0.216, p = 0.034, n = 17). b Correlation of circulating chemerin levels with OP glucose (r = 0.549, p = 0.023, n = 17). (PDF 44 kb