Inhibition of primordial germ cell proliferation by the medaka male determining gene Dmrt1bY-0

<p><b>Copyright information:</b></p><p>Taken from "Inhibition of primordial germ cell proliferation by the medaka male determining gene Dmrt1bY"</p><p>http://www.biomedcentral.com/1471-213X/7/99</p><p>BMC Developmental Biology 2007;7():99-99.</p><p>Published online 30 Aug 2007</p><p>PMCID:PMC2034567.</p><p></p>V:EGFP-SV40UTR control plasmid (A) while nuclear localized when transfected with pCMV:Dmrt1bY:GFP-SV40UTR construct (B). C and D: Radar histograms representing the DNA content distribution of Dmrt1bY:GFP relative to GFP (control) transfected cells expressed in percentage. "Ctr" and "GFP Ctr, GFP+" represent GFP negative and positive control cells in the plate tranfected with control GFP plasmid; similarly "Dmrt1bY, GFP+" and "Dmrt1b, GFP-" represent GFP negative and positive cells In the plate tranfected with Dmrt1bY:GFP plasmid. E and F: Cell cycle distribution reflected by DNA content in control stage 10 (MBT) and post-MBT (stage 13) -injected embryos

Inhibition of primordial germ cell proliferation by the medaka male determining gene Dmrt1bY-4

<p><b>Copyright information:</b></p><p>Taken from "Inhibition of primordial germ cell proliferation by the medaka male determining gene Dmrt1bY"</p><p>http://www.biomedcentral.com/1471-213X/7/99</p><p>BMC Developmental Biology 2007;7():99-99.</p><p>Published online 30 Aug 2007</p><p>PMCID:PMC2034567.</p><p></p> (A), female (B). Both sexes have a significantly higher number of PGCs in the right PGC lateral clump. C: Comparison of the PGC phenotype versus genotype (Dmrt1bY) at hatching stage. D: Offspring obtained by mating a Y/Yfemale with an Olvas X/Ymale. E: Comparison of the primordial gonad phenotype at hatching stage versus genotype (Yor Y). Identification of genetic sex in each individual was performed by PCR analysis for presence or absence after genomic DNA extraction. The fragments from either both Awr or Hd-rR-specific alleles were amplified with diagnostic primer sets. Scale bars: 7.5 μM

Inhibition of primordial germ cell proliferation by the medaka male determining gene Dmrt1bY-5

<p><b>Copyright information:</b></p><p>Taken from "Inhibition of primordial germ cell proliferation by the medaka male determining gene Dmrt1bY"</p><p>http://www.biomedcentral.com/1471-213X/7/99</p><p>BMC Developmental Biology 2007;7():99-99.</p><p>Published online 30 Aug 2007</p><p>PMCID:PMC2034567.</p><p></p>mordial gonad phenotype at hatching stage. B: male primordial gonad phenotype at hatching stage. C, D and E: fluorescence produced by BrdU incorporation colocalizes with GFP fluorescence of primordial germ cells in the Olvas transgenic line. GFP fluorescence (C), BrdU fluorescence (D), overlay (E). F: primordial gonad phenotype at hatching stage after Dmrt1bY morpholino injection in a genetic male