The Method of Analysis Aesthetic Paradigm of Dramatic Work

У статті зроблено огляд загальнонаукових підходів та методів, покладених в основу методики. Розкрито поняття естетичної парадигми літературного твору та зазначено етапи її дослідження. Обґрунтовано доцільність апробування методики на матеріалі драматичних творів. Визначено ключові та допоміжні естетичні категорії, наявні у драматичних творах. Розроблено методику аналізу естетичної парадигми драматичного твору. Представлено практичну реалізацію запропонованої методики.The review of scientific approaches and methods, fixed in basis of method is done in the article. The concept of aesthetical paradigm of literary work is exposed and the stages of its research are marked. Grounded expedience of assertion method on material of dramatic works. Certainly key and auxiliary aesthetical categories, present in dramatic works. The method of analysis of aesthetical paradigm of dramatic work is developed. Practical realization of the offered method is presented.Усова А. Ю. Методика аналізу естетичної парадигми драматичного твору / А. Ю. Усова // Науковий вісник Волинського національного університету імені Лесі Українки. Філологічні науки. Літературознавство. – Луцьк : Волин. нац. ун-т ім. Лесі Українки. – 2012. – № 13 (238). – С. 122-128

Bovine leukosis control measures

Bovine leukosis is one of the most common infectious diseases of farm animals, causing significant economic damage due to a decrease in production of livestock products, premature culling and slaughter of cows and servicing bulls. The disease needs special attention and control on behalf of the on-farm veterinarians and zootechnicians. The article briefly describes epizootic situation on bovine leukosis in the Russian Federation in 2004–2020. It also includes a report on the disease situation in “Sibirskaya Niva” LLC (the Irkutsk Oblast) for 2015–2021 and assesses effectiveness of health support and disease prevention measures taken on the farm. The paper gives a brief description of the agricultural establishment: its zoosanitary status as well as zootechnical, veterinary, therapeutic and preventive measures (disinsection, deratization, vaccination). Based on the data obtained, we found the ultimate cause of bovine leukosis on the farm: presumably, these were crossbred animals brought into the farm. In order to eradicate the disease, “Sibirskaya Niva” LLC has developed a plan on health support and disease prevention, which includes veterinary, zootechnical and economic measures. Thus, due to the actions taken from 2015 to 2019, the number of infected cows and heifers reduced by 6.42 and 2.78 times, correspondingly. At the same time, the overall number of infected animals decreased by 9.9%. Task-oriented measures taken by the state veterinary services made it possible to steadily reduce the number of infected animals by 2020. The comprehensive approach embracing the farm peculiarities has proven to be effective to quickly eliminate bovine leukosis, as the above-mentioned agricultural establishment exemplified it

Experimental research and assessment advantage of the benefits of using anxiolytics and neuroleptics in combination with antioxidant

The aim of the study – сomparative evaluation of the use of depression medications as a result of antioxidant poisoning and evaluation of the use of these drugs in their pure form in animals with chronic alcoholism.Цель исследования - сравнительная оценка использования депримирующих лекарственных средств в комбинации с антиоксидантом и их применения в чистом виде на животных со сформированной моделью хронического алкоголизма

TARGETED THERAPY OF NSCLC WITH EGFR ACTIVATING MUTATIONS IMPROVES THE QUALITY OF LIFE OF PATIENTS

Conversion of the incurable disease in the chronic form and improvement of the life quality parameters on the background of appearance of new drugs is of specific importance for patients with NSCLC with activating mutations. “ESMO thinks that treatment of patients with wide-spread and incurable cancer forms a greater share of the oncologists work… and for these patients the quality of life is of chief importance irrespective of its duration. Use of high-precision targeted anti- EGFR therapy allows considerably improving the quality of life of oncologic patients with non-small pulmonary carcinoma with activating mutations

Inactivation of Lactobacillus leichmannii ribonucleotide reductase by F2CTP: covalent modification

Ribonucleotide reductase (RNR) from Lactobacillus leichmannii, a 76 kDa monomer using adenosylcobalamin (AdoCbl) as a cofactor, catalyzes the conversion of nucleoside triphosphates to deoxynucleotides and is rapidly (<30 s) inactivated by 1 equiv of 2′,2′-difluoro-2′-deoxycytidine 5′-triphosphate (F[subscript 2]CTP). [1′-[superscript 3]H]- and [5-[superscript 3]H]F[subscript 2]CTP were synthesized and used independently to inactivate RNR. Sephadex G-50 chromatography of the inactivation mixture revealed that 0.47 equiv of a sugar was covalently bound to RNR and that 0.71 equiv of cytosine was released. Alternatively, analysis of the inactivated RNR by SDS−PAGE without boiling resulted in 33% of RNR migrating as a 110 kDa protein. Inactivation of RNR with a mixture of [1′-[superscript 3]H]F[subscript 2]CTP and [1′-[superscript 2]H]F[subscript 2]CTP followed by reduction with NaBH[subscript 4], alkylation with iodoacetamide, trypsin digestion, and HPLC separation of the resulting peptides allowed isolation and identification by MALDI-TOF mass spectrometry (MS) of a 3H/2H-labeled peptide containing C[subscript 731] and C[subscript 736] from the C-terminus of RNR accounting for 10% of the labeled protein. The MS analysis also revealed that the two cysteines were cross-linked to a furanone species derived from the sugar of F[subscript 2]CTP. Incubation of [1′-[superscript 3]H]F[subscript 2]CTP with C119S-RNR resulted in 0.3 equiv of sugar being covalently bound to the protein, and incubation with NaBH[subscript 4] subsequent to inactivation resulted in trapping of 2′-fluoro-2′-deoxycytidine. These studies and the ones in the preceding paper (DOI: 10.1021/bi9021318) allow proposal of a mechanism of inactivation of RNR by F[subscript 2]CTP involving multiple reaction pathways. The proposed mechanisms share many common features with F[subscript 2]CDP inactivation of the class I RNRs.National Institutes of Health (U.S.) (Grant number GM-29595