Managing the Gap Between Actual and Target Capital Structure: An Evidence From Pakistan

Investment framework is one of the most significant components that impact the company’s value. Reliable funding choices for a company generally lead to a capital structure that increases the firm’s value (Abor, 2006). Early studies provide contradictory reviews about a company’s capital structure decisions. This paper investigates the partial adjustment model for a company’s target capital structure. The study also explores how companies operating in different sectors of Pakistani market adjust towards the target capital structure levels. The study also recognizes that an unanticipated share price change also have an effect on the target capital structure. The results indicate that companies do have target leverage and that their adjustment speed varies from sector to sector of the Pakistani market. A typical sector closes more than 50% of the gap between its actual and its target debt ratios within one year

Rust resistance in faba bean (Vicia faba L.) : status and strategies for improvement

Faba bean (Vicia faba L.) is an important grain legume used as food and feed. Its production is threatened by abiotic stresses and diseases, of which rust (Uromyces viciae-fabae) is one of the major diseases in East and North Africa, China and the northern grain growing region of Australia. Understanding the genetic and physiological mechanisms of rust resistance in faba bean is in an early phase. The presence of seedling and adult plant resistance genes has been observed. The resistance most frequently utilised in applied plant breeding is race-specific, where the interaction between resistance genes in the host and avirulence genes in the pathogen confers resistance. The main drawback of using race-specific resistance is lack of durability, when deployed singly. Slow rusting or partial resistance, controlled by multiple genes of small effect, is generally non-race specific, so it can be more durable. We present the current knowledge of host resistance and pathogen diversity and propose rational breeding approaches aided with molecular markers to breed durable rust resistance in faba bean.Peer reviewe

Encephalitis Associated With Hemophagocytic Lymphohistiocytosis Secondary to Immune Checkpoint Inhibitors: An Unfamiliar Spin-Off.

Checkpoint inhibitors (CPI) have become mainstream in standard therapy in various tumors, especially in malignant melanoma. Despite their widespread beneficial effects, these inhibitors are also notorious for immune-related adverse events (irAEs). Hemophagocytic lymphohistiocytosis (HLH) is an aggressive and life-threatening syndrome of excessive immune activation. We report a case of a 33-year-old male having a history of metastatic melanoma on immunotherapy (status post two cycles of ipilimumab/nivolumab) admitted for persistent fever and elevated liver enzymes. Additional work showed anemia, thrombocytopenia, hypertriglyceridemia, and hyperferritinemia which meet the diagnostic criteria of histiocyte society HLH-2004. The patient was effectively treated with oral prednisone. Moreover, further complications encompassed slurred speech, word-finding difficulties, ataxia, and lower extremity hyperreflexia concerning for autoimmune encephalitis. He was treated with high-dose IV methylprednisolone (1 gram/day for 3 days) with improvement in symptoms. Autoimmune encephalitis associated with HLH can be fatal - high-dose IV methylprednisolone should be considered, but this avenue still needs to be explored

Efficacy of mannan-oligosaccharide and live yeast feed additives on performance, rumen morphology, serum biochemical parameters and muscle morphometric characteristics in buffalo calves

The objective of the current study was to assess the effect of dietary supplementations of mannan-oligosaccharide, live yeast, and a combination of these two additives on growth performance, histo-morphology of the rumen, and muscle morphometric attributes in buffalo calves. A total of twenty buffalo calves (average weight of 25 kg) having 3 months of age were distributed according to a complete randomized design. All animals were individually stalled in the shed and were fed ad-libitum. Experimental animals were divided into four groups for 67 days: Control group(without the inclusion of dietary supplementation); MOS group (Mannan oligosaccharide 5 g/clave/day; Yeast group (Live yeast 2g/calve/day) and Mixed group (MOS + Live Yeast 2.5g + 1g )/calve/day. Experimental results revealed that combined supplementation of MOS and Yeast and MOS alone resulted in an increased number of short-chain fatty acids in the rumen as well as ruminal pH (P<0.05). Results showed a significant improvement in average daily gain and FCR of MOS and Mixed supplemented groups (P<0.05). Histomorphological evaluation of rumen mucosal epithelium showed a significant improvement in the mixed-supplemented group (P<0.05) as compared to the yeast-supplemented and control groups. Muscle quality parameters such as meat texture showed significant improvement in MOS and mix-supplemented groups. Histological examination of longissimus dorsi muscle cross-section showed a significantly higher(P<0.05) muscle fiber and muscle fascicle diameter in both MOS and mix-supplemented calves groups. In conclusion, the results of this experiment revealed that the dietary addition of MOS, Live yeast, and their combination have positive effects on growth performance, rumen histology indices, and muscle morphometric features in buffalo calves.Comment: Pages 13, 4 figure

Does Performing Endoscopy Sooner Have an Impact on Outcomes in Patients With Acute Nonvariceal Upper Gastrointestinal Hemorrhage? A Systematic Review.

Background Endoscopy is the cornerstone for the diagnosis and treatment of nonvariceal upper gastrointestinal bleeding. Regarding the management of nonvariceal bleeding, the administration of crystalloid solution and proton pump inhibitors before endoscopy is well established, but the optimal timing of endoscopy has been a matter of debate and a subject of many investigational studies. The need for urgent endoscopy arises to provide prompt redress to acute bleeding, decrease the length of stay, and lower mortality from ongoing bleeding. Objective This study aimed to determine if endoscopy performed within 24 hours of presentation improves outcomes in terms of mortality, hospital length of stay, and rebleeding in individuals presenting with nonvariceal upper gastrointestinal bleed with any risk. Methodology We performed a systematic review of two large databases (PubMed and Google Scholar) to incorporate all studies published after 2000. We included studies with nonvariceal upper gastrointestinal bleeding and excluded those reporting variceal gastrointestinal hemorrhage. Results We reviewed eight studies that qualified after meeting our inclusion and exclusion criteria. We divided these studies into three separate groups based on the timing of endoscopy. Only two studies found a difference in mortality that was statistically significant in patients who underwent endoscopy within 24 hours of presentation. One study showed lower mortality in a patient who underwent urgent endoscopy, but it did not reach statistical significance. Other studies did not show any statistical difference in mortality, hospital length of stay, and rebleeding rates. The studies showed conflicting evidence on the amount of blood transfusion, though urgent endoscopy was found to be difficult in few studies due to blood obscuring the lesion. Conclusions While data suggest that there is a potential benefit in performing endoscopy sooner, there is no concrete evidence to point to a particular time range. Before performing endoscopy, the American Society for Gastrointestinal Endoscopy (2012) recommends adequate resuscitation with crystalloid solutions, blood transfusions, and antisecretory and prokinetic agent therapy. More investigational studies are needed to formulate a time-sensitive flow sheet to approach endoscopy in patients with nonvariceal upper gastrointestinal bleeding. A strict criterion is also needed to delineate patients into low-risk and high-risk groups. Doing so would provide a systematic approach to help with mortality, rebleeding, and healthcare resource utilization

DEVELOPMENT, FABRICATION, AND PERFORMANCE EVALUATION OF INDIRECT MULTI-PORTIONED TYPE SOLAR DRYER FOR AGRICULTURAL PRODUCE

OBJECTIVE: The primary objective of this study was to minimize post-harvest damages and improve the handling of agricultural commodities. The secondary objective was to assess the performance of the dryer by varying inlet velocity of air on temperature, final moisture of the product, drying period, and drying rate METHODS: The present research was carried out for the development, fabrication, and testing of a multi-portioned type solar dryer. The multi-portioned dryer consisted of a collector unit, air storage chamber, drying cabins, and a chimney. A fan assembly was also attached at the inlet of the collector and at the outlet of the drying compartment to control the air velocity and to remove the moist air. RESULTS: The data obtained from the experiments revealed that the maximum temperature elevation of 36.5oC was attained during a no-load test. The results obtained after carrying out load tests revealed that the maximum drying rate of 47 g/hr was achieved at the air inlet velocity of 3.2 m/s. The initial moisture content of 80.50% was brought down to 3.31% in five sunshine hours of 150 gram sliced potatoes. Each portion received air at varying speeds, and it was observed that the portion receiving air at the lowest speed had the maximum temperature. CONCLUSION: The lowest temperature elevation was recorded for the portion receiving air at the highest speed. The dryer was also found to be suitable for the on-field practical application for the drying of perishable commodities

Effect of combined siRNA of HCV E2 gene and HCV receptors against HCV

<p>Abstract</p> <p>Background/Aim</p> <p>Hepatitis C virus (HCV) is a major threat as almost 3% of the world's population (350 million individual) and 10% of the Pakistani population is chronically infected with this virus. RNA interference (RNAi), a sequence-specific degradation process of RNA, has potential to be used as a powerful alternative molecular therapeutic approach in spite of the current therapy of interferon-α and ribavirin against HCV which has limited efficiency. HCV structural gene E2 is mainly involved in viral cell entry via attachment with the host cell surface receptors i.e., CD81 tetraspanin, low density lipoprotein receptor (LDLR), scavenger receptor class B type 1 (SR-B1), and Claudin1 (CLDN1). Considering the importance of HCV E2 gene and cellular receptors in virus infection and silencing effects of RNAi, the current study was designed to target the cellular and viral factors as new therapeutic options in limiting HCV infection.</p> <p>Results</p> <p>In this study the potential of siRNAs to inhibit HCV-3a replication in serum-infected Huh-7 cells was investigated by combined treatment of siRNAs against the HCV E2 gene and HCV cellular receptors (CD81 and LDLR), which resulted in a significant decrease in HCV viral copy number.</p> <p>Conclusion</p> <p>From the current study it is concluded that the combined RNAi-mediated silencing of HCV E2 and HCV receptors is important for the development of effective siRNA-based therapeutic option against HCV-3a.</p

Acetone-Gasoline Blend as an Alternative Fuel in SI Engines: A Novel Comparison of Performance, Emission, and Lube Oil Degradation

The disproportionate use of petroleum products and stringent exhaust emissions has emphasized the need for alternative green fuels. Although several studies have been conducted to ascertain the performance of acetone-gasoline blends in spark-ignition (SI) engines, limited work has been done to determine the influence of fuel on lubricant oil deterioration. The current study fills the gap through lubricant oil testing by running the engine for 120 h on pure gasoline (G) and gasoline with 10% by volume acetone (A10). Compared to gasoline, A10 produced better results in 11.74 and 12.05% higher brake power (BP) and brake thermal efficiency (BTE), respectively, at a 6.72% lower brake-specific fuel consumption (BSFC). The blended fuel A10 produced 56.54, 33.67, and 50% lower CO, CO2, and HC emissions. However, gasoline remained competitive due to lower oil deterioration than A10. The flash-point and kinematic viscosity, compared to fresh oil, decreased by 19.63 and 27.43% for G and 15.73 and 20.57% for A10, respectively. Similarly, G and A10 showed a decrease in total base number (TBN) by 17.98 and 31.46%, respectively. However, A10 is more detrimental to lubricating oil due to a 12, 5, 15, and 30% increase in metallic particles like aluminum, chromium, copper, and iron, respectively, compared to fresh oil. Performance additives like calcium and phosphorous in lubricant oil for A10 decreased by 10.04 and 4.04% in comparison to gasoline, respectively. The concentration of zinc was found to be 18.78% higher in A10 when compared with gasoline. A higher proportion of water molecules and metal particles were found in lubricant oil for A10

Enviro-Economic Assessment of HHO–CNG Mixture Utilization in Spark Ignition Engine for Performance and Environmental Sustainability

Road transportation has received the attention of researchers due to its higher carbon footprint. Alternative fuels present major advantages in terms of environmental sustainability. For this reason, an enviro-economic analysis of alternative fuels carries great significance. However, scarce attempts have been rendered in order to ascertain the impact of a hydroxy gas (HHO) and compressed natural gas (CNG) mixture on sustainable environmental development. The current study addresses this issue by employing an HHO–CNG mixture and gasoline in spark ignition (SI) engines for the purposes of performance and environmental pollutants measurement. Then, engine emission data were substituted for Weibull distribution in order to establish suitability for 50 and 95% confidence intervals (CIs). The mixture outperformed gasoline in terms of brake-specific fuel consumption (BSFC) and emission contents. On average, hydroxy gas with CNG produced 10.59% lower oxides of nitrogen (NOX) comparative to gasoline. Finally, the enviro-economic analysis also turned out to be in favor of the hydroxy gas mixture owing to a saving of 36.14% in USD/year due to 27.87% lower production of carbon dioxide (CO2) emission

NS4A protein as a marker of HCV history suggests that different HCV genotypes originally evolved from genotype 1b

<p>Abstract</p> <p>Background</p> <p>The 9.6 kb long RNA genome of Hepatitis C virus (HCV) is under the control of RNA dependent RNA polymerase, an error-prone enzyme, for its transcription and replication. A high rate of mutation has been found to be associated with RNA viruses like HCV. Based on genetic variability, HCV has been classified into 6 different major genotypes and 11 different subtypes. However this classification system does not provide significant information about the origin of the virus, primarily due to high mutation rate at nucleotide level. HCV genome codes for a single polyprotein of about 3011 amino acids which is processed into structural and non-structural proteins inside host cell by viral and cellular proteases.</p> <p>Results</p> <p>We have identified a conserved NS4A protein sequence for HCV genotype 3a reported from four different continents of the world i.e. Europe, America, Australia and Asia. We investigated 346 sequences and compared amino acid composition of NS4A protein of different HCV genotypes through Multiple Sequence Alignment and observed amino acid substitutions C₂₂, V₂₉, V₃₀, V₃₈, Q₄₆and Q₄₇in NS4A protein of genotype 1b. Furthermore, we observed C₂₂and V₃₀as more consistent members of NS4A protein of genotype 1a. Similarly Q₄₆and Q₄₇in genotype 5, V₂₉, V₃₀, Q₄₆and Q₄₇in genotype 4, C₂₂, Q₄₆and Q₄₇in genotype 6, C₂₂, V₃₈, Q₄₆and Q₄₇in genotype 3 and C₂₂in genotype 2 as more consistent members of NS4A protein of these genotypes. So the different amino acids that were introduced as substitutions in NS4A protein of genotype 1 subtype 1b have been retained as consistent members of the NS4A protein of other known genotypes.</p> <p>Conclusion</p> <p>These observations indicate that NS4A protein of different HCV genotypes originally evolved from NS4A protein of genotype 1 subtype 1b, which in turn indicate that HCV genotype 1 subtype 1b established itself earlier in human population and all other known genotypes evolved later as a result of mutations in HCV genotype 1b. These results were further confirmed through phylogenetic analysis by constructing phylogenetic tree using NS4A protein as a phylogenetic marker.</p