TOXICITY AND ANTIMICROBIAL ACTIVITY FROM EXTRACT AND OLEANAN DERIVATIVE COMPOUNDS OF THE BARK MELOCHIA UMBELLATE (HOUTT) STAPF VAR. DEGRABRATA

Oleanan derivative compound namely 3-acetyl-12-oleanen-28-oic acid, has been isolated from n-hexane fraction of the bark M. umbellate (Houtt) Stapf var. degrabrata The\ud molecular structure was determined by IR spectroscopy, NMR 1D and 2D (1H-NMR, 13CNMR, DEPT, COSY, HMQC and HMBC). The results of test bioactivity to hexane,\ud chloroform, ethyl acetic, methanol extracts and compound 1 mentioned above showed\ud that they are toxic to A. salina (brine shrimp) with LC50 361.93 to 460 ??g/mL. The most\ud toxic one was the compound 1 with the LC50 value of 361.93 ??g/mL. At a concentration of 1000 ??g/mL, the extract of hexane, methanol and compound 1 showed very high\ud inhibition to the growth of B. subtilis and C.albican, whereas the extract of ethyl acetate\ud had very high inhibition effect to the growth of S. aureus and A. niger. The inhibition zone\ud of the three extracts and compound 1 were greater than 14 mm. On the other hand, the\ud extracts of chloroform gave weak inhibition against bacteria and fungi

Oleanan Derivate Compounds From Melochia umbellata (Houtt) Stapf var. Degrabrata K Bark And Their Bioactivity

Oleanan derivated 3-acetyl-12-oleanen-28-oat has been isolated from the n-hexane fraction of M. umbellata(Houtt) Stapf var. degrabrata K. bark. The molecular structure were determined by IR spectroscopy, 1D and 2DNMR (1H, 13C, DEPT, COSY, HMQC and HMBC) data analysis. Bioactivity tested results indicated that thecompound was toxic to A. salina with LC50 value was 361.93 mg/mL. At the concentration of 1000 mg/mL 3acetyl-12-oleanen-28-oat gave the highest inhibition to bacteria B. subtilis and the fungus C. albicans with the inhibited zone diameter was 15.8 mm and 15.2 mm, res

Isolasi, Karakterisasi, dan Uji Bioaktivitas Metabolit Sekunder dari Hydroid Aglaophenia Cupressina Lamoureoux sebagai Bahan Dasar Antimikroba

Isolation, structure elucidation, activity test against Salmonella thypi, Staphylococcus aureus of secondary metabolite from hydroid Aglaophenia cupressina Lamoureoux, were conducted. This study was aimed to identify and isolate the compound with antibacterial properties of hydroid Aglaophenia crupessina Lamoureoux. This study used stepwise method with the following sequences: extraction, isolation, purification, structure elucidation, and bioactivity test. Study results indicated three compounds found: (1)carboxylate acid compound: hexadecanoic acid and has antibacterial properties; (2) alkaloidcompound which was considered as a new compound: aglaounhas and has antimicrobialproperties; (3) steroid compound: β-sitosterol with no antimicrobial properties

Oleanan Derivate Compounds From Melochia umbellata (Houtt) Stapf var. Degrabrata K Bark And Their Bioactivity

Oleanan derivated 3-acetyl-12-oleanen-28-oat has been isolated from the n-hexane fraction of M. umbellata(Houtt) Stapf var. degrabrata K. bark. The molecular structure were determined by IR spectroscopy, 1D and 2DNMR (1H, 13C, DEPT, COSY, HMQC and HMBC) data analysis. Bioactivity tested results indicated that thecompound was toxic to A. salina with LC50 value was 361.93 mg/mL. At the concentration of 1000 mg/mL 3acetyl-12-oleanen-28-oat gave the highest inhibition to bacteria B. subtilis and the fungus C. albicans with the inhibited zone diameter was 15.8 mm and 15.2 mm, res

Oleanan Derivate Compounds From Melochia umbellata (Houtt) Stapf var. Degrabrata K Bark And Their Bioactivity

Oleanan derivated 3-acetyl-12-oleanen-28-oat has been isolated from the n-hexane fraction of M. umbellata (Houtt) Stapf var. degrabrata K. bark. The molecular structure were determined by IR spectroscopy, 1D and 2D NMR (1H, 13C, DEPT, COSY, HMQC and HMBC) data analysis. Bioactivity tested results indicated that the compound was toxic to A. salina with LC50 value was 361.93 mg/mL. At the concentration of 1000 mg/mL 3acetyl-12-oleanen-28-oat gave the highest inhibition to bacteria B. subtilis and the fungus C. albicans with the inhibited zone diameter was 15.8 mm and 15.2 mm, res

Buku ajar wawasan ipteks : menggunakan pendekatan learning

ix, 255 hlm.; 21 cm