Azimuthally invariant Mueller-matrix mapping of biological optically anisotropic network

A new technique of Mueller-matrix mapping of polycrystalline structure of histological sections of biological tissues is suggested. The algorithms of reconstruction of distribution of parameters of linear and circular dichroism of histological sections liver tissue of mice with different degrees of severity of diabetes are found. The interconnections between such distributions and parameters of linear and circular dichroism of liver of mice tissue histological sections are defined. The comparative investigations of coordinate distributions of parameters of amplitude anisotropy formed by Liver tissue with varying severity of diabetes (10 days and 24 days) are performed. The values and ranges of change of the statistical (moments of the 1st – 4th order) parameters of coordinate distributions of the value of linear and circular dichroism are defined. The objective criteria of cause of the degree of severity of the diabetes differentiation are determined

System of multifunctional Jones matrix tomography of phase anisotropy in diagnostics of endometriosis

The paper presents the results of Jones-matrix mapping of uterine wall histological sections with second-degree and third-degree endometriosis. The technique of experimental measurement of coordinate distributions of the modulus and phase values of Jones matrix elements is suggested. Within the statistical and cross-correlation approaches the modulus and phase maps of Jones matrix images of optically thin biological layers of polycrystalline films of plasma and cerebrospinal fluid are analyzed. A set of objective parameters (statistical and generalized correlation moments), which are the most sensitive to changes in the phase of anisotropy, associated with the features of polycrystalline structure of uterine wall histological sections with second-degree and third-degree endometriosis are determined

Complex degree of mutual anisotropy of biological liquid crystals nets

This paper is aimed to investigate the potentiality of describing and differentiating optical-anisotropic properties of biological liquid crystal net by statistic analysis of coordinate distributions of a new analytical parameter, a complex degree of mutual anisotrop

Phase maps for networks of polycrystalline human biological liquids: Statistical and fractal analyses

Performed in this work are complex statistical and fractal analyses of phase properties inherent to birefringence networks of liquid crystals consisting of opticallythin layers prepared from synovial fluid taken from human joints. Within the framework of a statistical approach, the authors have investigated values and ranges for changes of statistical moments of the 1-st to 4-th orders that characterize coordinate distributions for phase shifts between orthogonal components of amplitudes inherent to laser radiation transformed by synovial fluid layers for human joints with various pathologies. Using the Gramm-Charlie method, ascertained are correlation criteria for differentiation of phase maps describing pathologically changed liquid-crystal networks. In the framework of the fractal approach, determined are dimensionalities of self-similar coordinate phase distributions as well as features of transformation of logarithmic dependences for power spectra of these distributions for various types of human joint pathologies

Polarization-singular structure in laser images of phase-inhomogeneous layers to diagnose and classify their optical properties

Adduced in this work are the results of investigation aimed at analysis of coordinate distributions for azimuths and ellipticity of polarization (polarization maps) in laser images of three types of phase-inhomogeneous layers (PhIL), namely: rough, ground and bulk scattering layers. To characterize polarization maps for all the types of PhIL, the authors have offered to use three groups of parameters: statistical moments of the first to fourth orders, autocorrelation functions, logarithmic dependences for power spectra related to distributions of azimuths and ellipticity of polarization inherent to PhIL laser images. Ascertained are the criteria for diagnostics and classification of PhIL optical properties

Wavelet analysis for polarization maps of networks formed by liquid biological crystals in blood plasma: statistical and fractal approaches

Considered in this paper are the possibilities of local wavelet analysis for polarization-inhomogeneous images inherent to blood plasma of healthy and oncologically ill patients. Determined is the set of statistical, correlation and fractal parameters for distributions of wavelet coefficients that characterize different scales of polarization maps inherent to polycrystalline networks of amino-acids in blood plasma. Established are criteria for differentiation of processes that provide transformation of birefringent optically-anisotropic structures in blood plasma for various scales of their geometrical dimensions

Fluorescent Mueller-matrix polarimetry of biological tissues in differentiation of benign and malignant tumors

The work is aimed at investigation of diagnostic efficiency provided by a new azimuthally stable Mueller-matrix method for analyzing laser fluorescence coordinate distributions characterizing biological tissue histological sections. A new model of generalized optical anisotropy of biological tissues containing protein networks has been proposed in order to ascertain the processes of laser autofluorescence. The influence of complex mechanisms of both phase anisotropy (linear birefringence and optical activity) and linear (circular) dichroism was taken into account. The interrelations between the azimuthally stable Mueller-matrix elements characterizing laser autofluorescence and different mechanisms of optical anisotropy have been determined. The statistic analysis of coordinate distributions of these Mueller-matrix rotation invariants has been offered. Thereupon the quantitative criteria (statistic moments of the 1st to 4th orders) of differentiation of histological sections of uterus wall tumor – group 1 (dysplasia) and group 2 (adenocarcinoma) have been estimated

Azimuthally invariant laser polarimetry of polycrystalline smears of biological liquids

The model based on Mueller-matrix description of mechanisms providing optical anisotropy typical for polycrystalline smears of liquor (including optical activity, birefringence as well as linear and circular dichroism) has been suggested. Within the statistical analysis of such distributions, the objective criteria of differentiation of smears of liquor taken from dead patient at different moments after his death have been determined. From the viewpoint of probative medicine, the operational characteristics (sensitivity, specificity and accuracy) of the method of Mueller-matrix reconstruction of optical anisotropy parameters have been found and the efficiency of this method in another task – diagnostics of diseases of internal organs of rats – has been demonstrated

Scale-selective polarimetry of the birefringence distribution of myocardium tissue

In the series of research works the possibility of polarimetry diagnostic of optically anisotropic layers of biological tissues and fluids is demonstrated. This research aims to study fundamental potentiality of the new Stokespolarimetry approach to polarization-correlation mapping of microscopic images of ischemic heart disease and acute coronary insufficiency died patients myocardial tissue

Laser autofluorescent microscopy of histological sections of parenchymatous biological tissues of the dead

The results of laser autofluorescence microscopy of the distribution of the intensity of the MLA of polycrystalline structures of biological tissue preparations are presented. The data of a statistical analysis of the distribution of the magnitude of the intensity of MLA networks of biological crystals of histological sections of tissues of the spleen and kidney with the parenchymal morphological structure of the dead with different levels of blood loss are presented