Effect of Growth hormones for direct organogenesis in Bael (Aegle marmelos (L.) corr)

Aegle marmelos (L.) corr. commonly known as ‘Bael’ tree is included in the red- listed medicinal tree in South India. Riped and unripe fruits, root, bark and leaves of the tree are used in ayurvedic preparation for various ailments. This study was conducted to standardize the media for multiple shoot induction from different explants under in vitro condition. Among the various explants viz., meristematic tip, nodal bud, tender leaf, auxillary bud and cotyledon tried in different media, auxiliary bud had shown good response for shoot induction in 36 to 39 days. Among the various treatments viz., WPM supplemented with Benzyl Amino Purine (BAP) (0.5 to 3.0mg/lit), Kinetin (KN) (0.5 to 3mg/lit) and Indole acetic acid (IAA) (0.5 to 2mg/lit) for multiple shoot induction, the treatmental combination of WPM supplemented with BAP, KN and IAA, @ of each 0.5 mg/lit resulted in 7.75 shoots per cotyledon. The same combination, ½ strength of the medium with 1mg/lit BAP+2mg/lit NAA+ 1% AC resulted in root formation. Hence, as a means of in vitro conservation using cotyledon as explants microproagation is possible in bael

Study on the acceptance, complications and continuation rate of post-partum family planning using the post placental intrauterine contraceptive device among women delivering at a tertiary care hospital

Background: Post placental intrauterine contraceptive device use in India showed that most women were satisfied with their choice of immediate insertion of an IUCD and the rates of complications were relatively low. The large proportion of women accepting the method to limit future childbearing indicates the important place post placental IUCD hold. This study was done to evaluate the acceptance of intrauterine contraceptive device as an immediate family planning method following delivery. The complications associated with it were identified and continuation rates were assessed.Methods: Women admitted for delivery in a tertiary care hospital were included in the study. Only women who fulfilled the medical eligibility criteria were included in the study.Results: A total of 4209 women were counseled of which 780 (18.5%) women accepted the method, 3429 declined. Out of the 780 cases, 764 came for follow up, and 16 were lost to follow up, spontaneous expulsion was seen in 2 (0.2%) cases, removal was done on request in 1 case and continuation was seen in 761 (97.56%) women.Conclusions: The Post placental intrauterine contraceptive device is safe to insert immediately after delivery. It has good acceptance with minimal expulsion and very high continuation rates

Meckel-Gruber syndrome: a case report with review of literature

Meckel Gruber syndrome is an autosomal recessive disorder, characterized by a combination of renal cysts or cystic renal dysplasia, developmental anomalies of the central nervous system, hepatic dysgenesis and polydactyly. It is a rare syndrome with highest incidence in Gujarati Indians and Finnish population. We report a case of Meckel Gruber syndrome in non Gujarati Indian which was diagnosed on fetal autopsy

FORMULATION AND CHARACTERIZATION OF SUSTAINED RELEASE COATED MATRIX GRANULES OF METFORMIN HYDROCHLORIDE

Objective: Metformin hydrochloride is a biguanide antihyperglycemic agent which is a generally recommended first-line drug for the treatment of diabetes mellitus (Type II). The purpose of this investigation is to prepare sustained release matrix granules of metformin hydrochloride which are coated to extend the drug release over a longer time period.Methods: Metformin hydrochloride granules were prepared by mixing all the dry powders in a V-cone blender and wetting the powder mix with aqueous solution of hydroxypropyl methyl cellulose K100. The prepared granules (MG1-MG5) were investigated for drug release. The batch of granules which exhibited extended release for up to 4 h was coated in a standard coating pan with blends of Eudragit RS and RL to further enhance release period. These were marked as coated metformin granules (CMG3) and CMG4 which were later filled into empty capsules. The granules were characterized for micromeritic properties, percentage yield, particle size distribution, percentage of drug content, and in vitro release of the drug.Results: All the formulations showed percentage yield in the range of 77.66–82.86% and drug content in the range of 78.23–96.62%. CMG3 showed drug release of 97.02% for 12 h. Fourier-transform infrared spectroscopy and differential scanning calorimetry studies indicated that no possible interaction existed between the drug and the polymers used. Scanning electron microscopy images revealed that the granules were spherical in shape with smooth surface and completely covered with a coating of polymer. Kinetic analysis of drug release confirmed that drug release followed zero-order kinetics where it is independent of the concentration.Conclusion: From the results, it was analyzed that design of coated granules employing the polymers used in the present work can produce a sustained release of the drug over a period of 12 h

COMPARISON AND CHARACTERIZATION OF INCLUSION COMPLEXES AND SOLID DISPERSIONS IN ENHANCEMENT OF DISSOLUTION RATE OF POORLY WATER SOLUBLE DRUG

Objective: The purpose of the present study was to enhance solubility and dissolution characteristics of indomethacin by preparing inclusion complexes with hydroxypropyl β-cyclodextrin (HP β-CD) and solid dispersions with PEG 6000 to enhance its in vitro drug release and to further formulate it as a tabletMethods: Solid dispersions (SDs) and inclusion complexes (ICs) of Indomethacin with PEG 6000 and HP β-CD respectively were prepared to enhance the dissolution rate of this poorly water-soluble drug belonging to BCS class II. A comparison was made between two systems: solid dispersions with PEG 6000 obtained using melting and solvent evaporation technique, inclusion complexes with HP β-CD prepared by kneading technique. SDs were prepared in 1:1, 1:2, 1:3 and ICs in 1:0.25, 1:0.5, 1:1 w/w ratios of drug: polymer. Both the systems were characterized by FTIR, SEM, DSC, X-RD.Results: The dissolution of indomethacin increased with the increase in the concentration of the polymers. F4 and F9 formulations showed complete drug release in less than 30 min. Dissolution studies indicated that cyclodextrin complexes showed a better enhancement of dissolution rate when compared to solid dispersions. CDs were found to be more effective than PEGs at lower concentrations. These formulations were further compressed as tablets.Conclusion: The FTIR and DSC studies showed that no interactions existed between the drug and the polymer

HDR Image Quality Assessment using Tone Mapping Operators

With the developments in Image acquisition techniques there is an increasing interest towards High Dynamic Range (HDR) images where the number of intensity levels ranges between 2 to 10,000. With these many intensity levels, the accurate representation of luminance variations is absolutely possible. But, because the standard display devices are devised to display Low Dynamic Range (LDR) images, there is a need to convert HDR image to LDR image without losing important image structures in HDR images. In the literature a number of techniques are proposed to get tone mapped image from HDR image. In this paper an attempt has been made to analyze four TMOs, i.e., Linear Mode, Gamma Correction, Reinhard and Reinhard with color correction. A subjective analysis tells us that the Reinhard with color correction has produced better results. In this paper two new tone mapping operators are proposed

Correlation of levels of serum C reactive protein in second trimester with fetomaternal outcome

Background: The aim of the study was to evaluate the association between levels of second trimester C Reactive protein (CRP) with adverse pregnancy outcomes.Methods: Present study was a prospective analytical study carried out among 359 pregnant women between 14-18 weeks. All were primigravida, who attended antenatal OPD at RGGWCH between January 2019 to June 2020. All subjects fulfilled inclusion and exclusion criteria. Informed written consent was obtained in each case. A detailed history, clinical and ultrasound examination was done to confirm the gestational age. Then blood was collected and sent for serum CRP estimation. Patients were followed upto discharge of the mother and baby and fetomaternal outcome studied. Once the sample size was attained, the correlation between CRP levels and fetomaternal outcome was studied. Results: Increased levels of serum CRP in early second trimester in primigravida were significantly associated with adverse pregnancy outcomes like preterm delivery (p value 0.001), PROM (premature rupture of membranes) (p value 0.01) and low APGAR (p value 0.01).Conclusions: Early detection of higher levels of serum CRP in pregnancy can be used as predictor of inflammation and may help in early intervention, follow up and timely referral of the patients to minimize adverse maternal and fetal complications.

Effect of Granulation Methods on Drug Release Studies in Sustained Release Matrix Tablets of a Poorly Soluble Drug prepared using Synthetic Polymers

Vomiting or emesis is the abnormal emptying of stomach and upper part of intestine through esophagus and mouth. It occurs due to stimulation of the emetic (vomiting) centre situated in the medulla oblongata. Domperidone, a D2 receptor antagonist has antiemetic and prokinetic action is used as a model drug in the present work to prepare Sustained release matrix tablets using various synthetic polymers like Eudragit and HPMC K15 M. The tablets are designed to have a pH dependent release profile in order to prevent initial drug release in the stomach to reduce the possible gastro-irritant and ulcerogenic effects of the drug. Different polymer and diluent concentrations and various compression techniques like wet granulation technique and direct compression techniques were used in order to release the contents of the tablets in a sustained manner over a certain period of time. Domperidone is BCS Class II drug and its solubility was enhanced by preparing solid dispersions using solvent evaporation technique. In the present work solid dispersions containing drug and polymer mixture in the ratio 1:1 was further formulated into tablets by incorporating various synthetic polymers in three different concentrations. The tablets were prepared using different granulating techniques. Formulation (F3) containing drug and  polymers in the ratio 1:1 prepared by wet granulation technique could sustain the drug release over a period of 12h and hence considering all the post compression parameters it was optimized as the better formulation. FTIR, DSC, X-Ray Diffraction, SEM studies were performed for optimized solid dispersion mixture and also the optimized formulation. Keywords: Solubility enhancement, Solid dispersions, Solvent Evaporation, Wet granulation, Direct Compression

Formulation and evaluation of atenolol oro dispersable tablets by co-processed super-disintegration process

Oral disintegrating tablet (ODT) is defined as A solid dosage form containing medical substances or active ingredient which disintegrates rapidly usually within a matter of seconds when placed upon the tongue. The aim of the present research is to formulate Atenolol oral disintegrating tablets.Atenolol is ?1- cardio selective adrenergic receptor blocker, widely used in the treatment of hypertension, angina pectoris, arrhythmias and myocardial infarction. It works by slowing down the heart and reducing the work load of the heart.Atenolol was specifically developed so as to pass the blood brain barrier and overcome theside effects such as depression and nightmares.It has been reported that atenolol undergo extensive hepatic first pass metabolism following oral administration and has shorter biological half-life of 6 7 hours with oral bioavailability of 50%. The conventional tablets of atenolol are reported to exhibit fluctuations in the plasma drug levels after administration. Atenolol ODTs are prepared by novel co-processed super-disintegration process using Cross Povidone and Cross carmellose sodium, as the super disintegrants. The prepared tablets were characterized for their hardness, weight variation, disintegration time, wetting time, water absorption ratio friability, and in vitro dissolution studies.he ability of the tablet to release the drug faster depends on the concentration and type of super disintegrant. In this study the oral disintegrating tablets containing Cross carmellose sodium and Cross Povidone as the super disintegrant in the ratio of 1:1 shows better release of drug. About 99.5% of the drug was released from the tablets in 6 mins. Therefore, based on the physico chemical properties, in vitro drug release profile and mouth feel formulation F 1 containing 1:1 of Cross carmellose sodium and crospovidone is optimised as the best formulation

Assessment of oxidative stress and antioxidant status in cattle infected with Theileria orientalis

Oriental theileriosis is a major haemoprotozoan disease of cattle and causes huge economic losses to the farmers. Oxidative stress plays an important role in the development of anaemia and subsequent complications associated with theileriosis. The present study was conducted to assess oxidative stress and antioxidant status of cattle infected with Theileria orientalis. In the present study, 16 animals which were positive for theileriosis by blood smear examination were selected. Confirmation of oriental theileriosis was done with polymerase chain reaction (PCR). Assessment of oxidative stress and antioxidant status were done by measuring malondialdehyde (MDA) levels and reduced glutathione activity respectively. Both MDA levels and reduced glutathione activity did not indicate any significant difference in animals infected with T. orientalis from control animals