Additional file 5: Figure S5. of The anti-inflammatory Annexin A1 induces the clearance and degradation of the amyloid-β peptide

Correlation of ANXA1 expression with FPR, cytokines and Aβ. A. Scatterplot showing relationship between FPRL1/FPR2 and ANXA1 protein expression assessed by Western blotting in the frontal cortex of neurologically healthy controls and sporadic Alzheimer’s patients (n = 5 controls, 3 males, 2 females, range 81–97 years, mean age 86.8 ± 3 years; n = 7 AD cases, 3 males, 4 females, range 83–98 years, mean age 91.3 ± 2 years). B. Scatterplot showing relationship between FPRL1/FPR2 and ANXA1 protein expression assessed by Western blotting in the motor cortex of 5XFAD mice and wild-type littermates and (n = 6/group, males aged 12 and 26 weeks). C. Scatterplot showing relationship between FPR rs1 and ANXA1 mRNA expression assessed in the frontal cortex of 5XFAD mice and wild-type littermates by qPCR (n = 19, males aged 12 and 26 weeks). D. Scatterplot showing relationship between ANXA1 protein expression assessed by Western blotting and TNFα expression measured by ELISA in in the frontal cortex of neurologically healthy controls and sporadic Alzheimer’s patients (n = 8 controls, 5 males, 3 females, range 40–82 years, mean age 67.1 ± 6 years; n = 10 AD cases, 7 males, 3 females, range 42–98 years, mean age 72.5 ± 6 years). E. Scatterplot showing relationship between Tnf and ANXA1 mRNA expression assessed in the frontal cortex of 5XFAD mice and wild-type littermates by qPCR (n = 18, males aged 12 and 26 weeks). ***p < 0.001, r 2 = 0.5779. F. Scatterplot showing relationship between Il6 and ANXA1 mRNA expression assessed in the frontal cortex of 5XFAD mice and wild-type littermates by qPCR (n = 19, males aged 12 weeks and 26 weeks). ****p < 0.0001, r 2 = 0.6352. G. Scatterplot showing relationship between ANXA1 protein expression assessed by Western blotting and Aβ1–40 expression measured by ELISA in the frontal cortex of neurologically healthy controls and sporadic Alzheimer’s patients (n = 10 controls, 6 males, 4 females, range 40–97 years, mean age 71.5 ± 6 years; n = 12 AD cases, 8 males, 4 females, range 42–98 years, mean age 74.4 ± 5 years). H. Scatterplot showing relationship between Aβ and ANXA1 protein expression assessed by Western blotting in the cortex of 5XFAD mice (n = 11, males aged 12 and 26 weeks). (PDF 111 kb

Additional file 3: Figure S3. of The anti-inflammatory Annexin A1 induces the clearance and degradation of the amyloid-β peptide

ImageStream quantification of 5-FAM-labelled Aβ1–42 phagocytosis by BV2 cells. Histogram showing gating of BV2 cells. B. Scatterplot showing gating of focused BV2 cells. C. Histogram and representative images of negative and positive 5-FAM-labelled Aβ1–42 phagocytosis by BV2 cells. D. Representative histograms of 5-FAM-labelled Aβ1–42 phagocytosis by BV2 cells (WT, control shRNA and shRNA ANXA1) incubated for 3 h with 5-FAM-labelled Aβ1–42 (5 μg/ml) added to BV2-conditioned medium. (PDF 865 kb

Весь Тагил. 2011. № 006

Efficiency of ANXA1 shRNA infection in BV2 cells. Representative Western blot image showing protein expression of ANXA1 in BV2 cells infected with control shRNA, and ANXA1 shRNA—clones 492A, 492B, 495A and 495B. Band intensities were determined using ImageJ and normalised to tubulin. (PDF 99 kb

Additional file 2: of The anti-inflammatory Annexin A1 induces the clearance and degradation of the amyloid-β peptide

Supplementary methods. (DOC 22 kb

Flow chart of patient recruitment to each sub-trial with sample size for each group randomised

<p><b>Copyright information:</b></p><p>Taken from "Design of a multicentre randomized trial to evaluate CT colonography versus colonoscopy or barium enema for diagnosis of colonic cancer in older symptomatic patients: The SIGGAR study"</p><p>http://www.trialsjournal.com/content/8/1/32</p><p>Trials 2007;8():32-32.</p><p>Published online 27 Oct 2007</p><p>PMCID:PMC2174515.</p><p></p