Arctic Acoustic Environments – Federating observations and analyses with the International Quiet Ocean Experiment

Arctic waters are experiencing rapid changes due to global warming, affecting ecosystems and leading to increasing economic activities. Many of these changes can be measured directly or indirectly with underwater acoustics. The Working Group on the Arctic Acoustic Environment (AAE) of the International Quiet Ocean Experiment (IQOE) aims to stimulate observations of sound (levels and distribution) in the Arctic Ocean and its impacts. We organised a virtual conference in November 2020 to share recent results from the international community and discuss common issues and possible solutions. The COVID-19 pandemic amplified the challenges of Arctic deployments and recoveries, curtailing access to ships at very short notice, but also opening the way for more direct collaboration. The post-COVID task will be to establish more resilient international back-up mechanisms for Arctic operations to support acoustic (and other) observations. The increasing length of the measurements, spanning several decades now, with sampling rates often close to 100,000 samples/second, results in “big data” challenges of storage, sharing, data retrieval and long-term archiving. Discussions also addressed the emerging trends in acoustic propagation models across complex terrains and machine learning (with the need for accessibility and traceability). Finally, the embedding of local and traditional knowledge must be accomplished through dialogue and co-ownership of the science and results

Potential of Radiotelescopes for Atmospheric Line Observations: I. Observation Principles and Transmission Curves for Selected Sites

Existing and planned radiotelescopes working in the millimetre (mm) and sub-millimetre wavelengths range provide the possibility to be used for atmospheric line observations. To scrutinize this potential, we outline the differences and similarities in technical equipment and observing techniques between ground-based aeronomy mm-wave radiometers and radiotelescopes. Comprehensive tables summarizing the technical characteristics of existing and future (sub)-mm radiotelescopes are given. The advantages and disadvantages using radiotelescopes for atmospheric line observations are discussed. In view of the importance of exploring the sub-mm and far-infrared wavelengths range for astronomical observations and atmospheric sciences, we present model calculations of the atmospheric transmission for selected telescope sites (DOME-C/Antarctica, ALMA/Chajnantor, JCMT and CSO on Mauna Kea/Hawaii, KOSMA/Swiss Alpes) for frequencies between 0 and 2000 GHz (150 micron) and typical atmospheric conditions using the forward model MOLIERE (version~5). For the DOME-C site, the transmission over a larger range of up to 10 THz (30 micron) is calculated in order to demonstrate the quality of an earth-bound site for mid-IR observations. All results are available on a dedicated webpage (http://transmissioncurves.free.fr)Comment: Planetary and Space Science accepted (in press), see also website http://transmissioncurves.free.f

Interaction modes of microsomal cytochrome p450s with its reductase and the role of substrate binding

The activity of microsomal cytochromes P450 (CYP) is strictly dependent on the supply of electrons provided by NADPH cytochrome P450 oxidoreductase (CPR). The variant nature of the isoform-specific proximal interface of microsomal CYPs implies that the interacting interface between the two proteins is degenerated. Recently, we demonstrated that specific CPR mutations in the FMN-domain (FD) may induce a gain in activity for a specific CYP isoform. In the current report, we confirm the CYP isoform dependence of CPR’s degenerated binding by demonstrating that the effect of four of the formerly studied FD mutants are indeed exclusive of a specific CYP isoform, as verified by cytochrome c inhibition studies. Moreover, the nature of CYP’s substrate seems to have a modulating role in the CPR:CYP interaction. In silico molecular dynamics simulations of the FD evidence that mutations induces very subtle structural alterations, influencing the characteristics of residues formerly implicated in the CPR:CYP interaction or in positioning of the FMN moiety. CPR seems therefore to be able to form effective interaction complexes with its structural diverse partners via a combination of specific structural features of the FD, which are functional in a CYP isoform dependent manner, and dependent on the substrate bound.publishersversionpublishe

The ladybird homeobox genes are essential for the specification of a subpopulation of neural cells

AbstractIn Drosophila, neurons and glial cells are produced by neural precursor cells called neuroblasts (NBs), which can be individually identified. Each NB generates a characteristic cell lineage specified by a precise spatiotemporal control of gene expression within the NB and its progeny. Here we show that the homeobox genes ladybird early and ladybird late are expressed in subsets of cells deriving from neuroblasts NB 5-3 and NB 5-6 and are essential for their correct development. Our analysis revealed that ladybird in Drosophila, like their vertebrate orthologous Lbx1 genes, play an important role in cell fate specification processes. Among those cells that express ladybird are NB 5-6-derived glial cells. In ladybird loss-of-function mutants, the NB 5-6-derived exit glial cells are absent while overexpression of these genes leads to supernumerary glial cells of this type. Furthermore, aberrant glial cell positioning and aberrant spacing of axonal fascicles in the nerve roots observed in embryos with altered ladybird function suggest that the ladybird genes might also control directed cell movements and cell–cell interactions within the developing Drosophila ventral nerve cord

The Role of the FMN-Domain of Human Cytochrome P450 Oxidoreductase in Its Promiscuous Interactions With Structurally Diverse Redox Partners

[This corrects the article DOI: 10.3389/fphar.2020.00299.].publishersversionpublishe

Single Mutations in Cytochrome P450 Oxidoreductase Can Alter the Specificity of Human Cytochrome P450 1A2-Mediated Caffeine Metabolism

Funding Information: F.E. and M.K.: UID/BIM/0009/2020 of the Portuguese Fundação para a Ciência e a Tecnologia (FCT) and HLTH-2022-STAYHLTH-02/grant agreement 101095679 of the European Horizon´s research and innovation program. Publisher Copyright: © 2023 by the authors.A unique cytochrome P450 (CYP) oxidoreductase (CPR) sustains activities of human microsomal CYPs. Its function requires toggling between a closed conformation enabling electron transfers from NADPH to FAD and then FMN cofactors and open conformations forming complexes and transferring electrons to CYPs. We previously demonstrated that distinct features of the hinge region linking the FAD and FMN domain (FD) modulate conformer poses and their interactions with CYPs. Specific FD residues contribute in a CYP isoform-dependent manner to the recognition and electron transfer mechanisms that are additionally modulated by the structure of CYP-bound substrate. To obtain insights into the underlying mechanisms, we analyzed how hinge region and FD mutations influence CYP1A2-mediated caffeine metabolism. Activities, metabolite profiles, regiospecificity and coupling efficiencies were evaluated in regard to the structural features and molecular dynamics of complexes bearing alternate substrate poses at the CYP active site. Studies reveal that FD variants not only modulate CYP activities but surprisingly the regiospecificity of reactions. Computational approaches evidenced that the considered mutations are generally in close contact with residues at the FD–CYP interface, exhibiting induced fits during complexation and modified dynamics depending on caffeine presence and orientation. It was concluded that dynamic coupling between FD mutations, the complex interface and CYP active site exist consistently with the observed regiospecific alterations.publishersversionpublishe

RENO, a European Postmarket Surveillance Registry, confirms effectiveness of coronary brachytheraypy in routine clinical practice.

Purpose: To assess, by a European registry trial, the clinical event rate in patients with discrete stenotic lesions of coronary arteries (de novo or restenotic) in single or multiple vessels (native or bypass grafts) treated with -radiation. Methods and Materials: Between April 1999 and September 2000, 1098 consecutive patients treated in 46 centers in Europe and the Middle East with the Novoste Beta-Cath System were included in Registry Novoste (RENO). Results: Six-month follow-up data were obtained for 1085 patients. Of 1174 target lesions, 94.1% were located in native vessels and 5.9% in a bypass graft; 17.7% were de novo lesions, 4.1% were restenotic, and 77.7% were in-stent restenotic lesions. Intravascular brachytherapy was technically successful in 95.9% of lesions. Multisegmental irradiation, using a manual pullback stepping maneuver to treat longer lesions, was used in 16.3% of the procedures. The in-hospital rate of major adverse cardiac events was 1.8%. At 6 months, the rate was 18.7%. Angiographic follow-up was available for 70.4% of the patients. Nonocclusive restenosis was seen in 18.8% and total occlusion in 5.7% of patients. A combined end point for late (30–180 days) definitive or suspected target vessel closure was reached in 5.4%, but with only 2% of clinical events. Multivariate analysis was performed for major adverse cardiac events and late thrombosis. Conclusion: Data obtained from the multicenter RENO registry study, derived from a large cohort of unselected consecutive patients, suggest that the good results of recent randomized controlled clinical trials can be replicated in routine clinical practice. © 2003 Elsevier Science Inc

Логическое управление и технологические защиты : методические указания к практическим работам

В пособии представлены практические задания по дисциплине «Логическое управление и защиты» Каждая работа содержит краткое изложение теоретических сведений, разобранные примеры выполнения заданий и варианты для самостоятельной индивидуальной проработки изученного материала. В практических работах затрагиваются законы алгебры логики Буля, основные логические элементы, известные методы построения и минимизации логических схем, а также вопросы синтеза и анализа конечных логических автоматов. Предназначено для магистров, обучающихся по направлению 13.04.01 «Теплоэнергетика и теплотехника»

The Molecular Fingerprint of Fluorescent Natural Organic Matter Offers Insight into Biogeochemical Sources and Diagenetic State

Investigating the biogeochemistry of dissolved organic matter (DOM) requires the synthesis of data from several complementary analytical techniques. The traditional approach to data synthesis is to search for correlations between measurements made on the same sample using different instruments. In contrast, data fusion simultaneously decomposes data from multiple instruments into the underlying shared and unshared components. Here, Advanced Coupled Matrix and Tensor Factorization (ACMTF) was used to identify the molecular fingerprint of DOM fluorescence fractions in Arctic fjords. ACMTF explained 99.84% of the variability with six fully shared components. Individual molecular formulas were linked to multiple fluorescencecomponents and vice versa. Molecular fingerprints differed in diversity and oceanographic patterns, suggesting a link to the biogeochemical sources and diagenetic state of DOM. The fingerprints obtained through ACMTF were more specific compared to traditional correlation analysis and yielded greater compositional insight. Multivariate data fusion aligns extremely complex, heterogeneous DOM data sets and thus facilitates a more holistic understanding of DOM biogeochemistry

Initial experience using the Palmaz Corinthian stent for right ventricular outflow obstruction in infants and small children

The original Palmaz balloon expandable stent has been used extensively for the treatment of vascular stenoses in older children and young adults. Placement of the Palmaz stent in infants and small children, however, is limited by stent inflexibility, large delivery sheath size, and concerns about creating fixed obstructions after the placement of small diameter stents in growing patients. New Palmaz Corinthian stents were placed through 6 French sheaths in four high-risk patients with postoperative right ventricular outflow obstruction. Patients were not considered candidates for surgical repair. Median patient age and weight were 17 months (range 5–32 months) and 7.7 kg (range 4.6–11.1 kg), respectively. Median fluoroscopy time was 58.2 min (range 55.2–172 min). No complications were encountered. In each case, successful stent placement was achieved, and surgery with cardiopulmonary bypass was avoided. Palmaz Corinthian stents are more flexible, require a smaller delivery sheath, have equal or increased radial strength, and can be maximally expanded to a greater cross sectional area when compared to the original Palmaz stent. These characteristics make the Palmaz Corinthian stent a reasonable alternative for use in a select group of infants and small children who are not candidates for surgical repair of postoperative right ventricular outflow obstruction. Cathet. Cardiovasc. Intervent. 51:444–449, 2000. © 2000 Wiley-Liss, Inc.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/35251/1/14_ftp.pd