Developing procedures for assessment of ecological status of Indian River basins in the context of environmental water requirements

River basins / Ecology / Indicators / Environmental flows / Environmental management / Habitats / Biota / Fish / Ecosystems / India / Krishna River Basin / Chauvery River Basin / Narmada River Basin / Periyar River Basin / Ganga River Basin

Studies on secondary metabolites of Som ( Persea bombycina Kost), a primary host plant of muga silkworm (Antheraea assamensis Helfer)

The host plant selection behaviour or feeding preferences of the insects are largely mediated by the presence and distribution of secondary metabolites in plants. Quantitative estimation of few secondary metabolites and HPLC analysis of phenolic compounds were carried out from the leaves of Som plant (Persea bombycina Kost) which is a primary metabolite of the shimmering yellow golden silk yarn producer, the muga silkworm (Antheraea assamensis Helfer). β –sitosterol content in the tender leaves was significantly the highest (1.06%) over semi-mature or mature leaves, whereas Chlorogenic acid content was more in medium leaves (2.06%). Phytic acid content was significantly higher in mature leaves (2310.05 mg/100g), total phenol in tender leaves (1.946%), ADF in mature leaves (26.50%), ADL in mature leaves and tannin in tender leaves (6.71%). Purified phenolic extract when subjected to HPLC analysis, 6 prominent peaks were observed and screening with standard indicates Peaks 1, 2, 3 and 6 correspond to Chlorogenic acid, catechol, Morin and gallic acid, respectively. Keywords: Som, Persea bombycina, Muga silkworm, Antheraea assamensis, secondary metabolites, HPLC analysis

Induced immunity in Antheraea assama Ww larvae against flacherie causing Pseudomonas aeruginosa AC-3

This study reports for the first time the induction of immunity in Antheraea assama Ww larvae against bacterial flacherie. In silkworms group of disease caused by bacteria are collectively called 1Cflacherie. 1D This refers to the flaccid condition of the larvae due to the infections of bacterial strains pathogenic to muga silkworm. Antibacterial activity against pathogenic Pseudomonas aeruginosa AC-3 causing flacherie, was induced by injection of heat-killed cells of the same strain. Experiments on larval survivability and viable cell count revealed peak immune response on third day. Comparison of the amount of food ingested, excreta produced and larval weight of the salineinjected control, live bacteria-challenged larvae and heat-killed bacteria-injected larvae 1C(vaccinated) 1D confirmed the development of immunity against bacterial infection in the 1Cvaccinated 1D set. The haemolymph of A. assama larvae was analyzed for proteins associated with bacterial infection. Out of the total 32 detected proteins, eleven (A1 132, A15 1320, A22 1323, and A29) were constitutively synthesized in both the control and live bacteria-injected larvae. Four inducible proteins A4, A9 1310, and A21 were detected in the haemolymph of the live bacteria-injected larvae. Synthesis of rest of the proteins varied between the control and their live bacteria-injected counterparts. General protein profile of 1Cvaccinated 1D larvae injected with live bacteria were found to be similar to that of the saline-injected control. Author Keywords: Antheraea assama; Flacherie; Pseudomonas aeruginosa; Immunity; Haemolymph proteins Article Outline 1. Introduction 2. Materials and methods 2.1. Rearing of silkworms 2.2. Induction of disease symptoms and immunity 2.3. Determination of time period for peak immune response 2.4. Feeding efficiency 2.5. Haemolymph preparation 2.6. Comparative antibacterial activity of immune and normal haemolymph 2.7. SDS 13PAGE of haemolymph 3. Results 4. Discussion Acknowledgements Reference

Osteoid osteoma of the ethmoid bone associated with dacryocystitis

BACKGROUND: Osteoid osteomas (OO) are small, benign osteoblastic lesions. Ethmoid bone OO has been very rarely reported so far. CASE PRESENTATION: We report a case of a 16-year-old boy suffering from persistent epiphora and a mild pain in the area of median canthus, due to a bone density mass within the right ethmoid air cells extending to the ipsilateral right orbit. The mass was removed via an external ethmoidectomy approach. Histopathologic examination of the specimen set the diagnosis of OO. One year after the operation the patient is free of symptoms, while no recurrence occurred. CONCLUSION: A case of ethmoid bone OO associated with dacryocystitis is reported. Although benign and rare, OO should be considered in differential diagnosis of the ethmoid bone osteoblastic lesions

Nanoscale transient magnetization gratings excited and probed by femtosecond extreme ultraviolet pulses

We utilize coherent femtosecond extreme ultraviolet (EUV) pulses derived from a free electron laser (FEL) to generate transient periodic magnetization patterns with periods as short as 44 nm. Combining spatially periodic excitation with resonant probing at the dichroic M-edge of cobalt allows us to create and probe transient gratings of electronic and magnetic excitations in a CoGd alloy. In a demagnetized sample, we observe an electronic excitation with 50 fs rise time close to the FEL pulse duration and ~0.5 ps decay time within the range for the electron-phonon relaxation in metals. When the experiment is performed on a sample magnetized to saturation in an external field, we observe a magnetization grating, which appears on a sub-picosecond time scale as the sample is demagnetized at the maxima of the EUV intensity and then decays on the time scale of tens of picoseconds via thermal diffusion. The described approach opens prospects for studying dynamics of ultrafast magnetic phenomena on nanometer length scales