Anthriscus nemorosa essential oil inhalation prevents memory impairment, anxiety and depression in scopolamine-treated rats

Anthriscus nemorosa (Bieb.) Sprengel is used for medicinal purposes in traditional medicine around the world, including Turkey. Ethnobotanical studies suggest that Anthriscus essential oil could improve memory in Alzheimer’s disease. The current study was hypothesized to investigate the beneficial effects of inhaled Anthriscus nemorosa essential oil on memory, anxiety and depression in scopolamine-treated rats. Anthriscus nemorosa essential oil was administered by inhalation in the doses of 1% and 3% for 21 continuous days and scopolamine (0.7 mg/kg) was injected intraperitoneally 30 min before the behavioral testing. Y-maze and radial arm-maze tests were used for assessing memory processes. Also, the anxiety and depressive responses were studied by elevated plus-maze and forced swimming tests. As expected, the scopolamine alone-treated rats exhibited the following: decrease the percentage of the spontaneous alternation in Y-maze test, increase the number of working and reference memory errors in radial arm-maze test, decrease of the exploratory activity, the percentage of the time spent and the number of entries in the open arm within elevated plus-maze test and decrease of swimming time and increase of immobility time within forced swimming test. However, dual scopolamine and Anthriscus nemorosa essential oil-treated rats showed significant improvement of memory formation and exhibited anxiolytic- and antidepressant-like effects in scopolamine-treated rats. These results suggest that Anthriscus nemorosa essential oil inhalation can prevent scopolamineinduced memory impairment, anxiety and depression.Emel Aydin Akbaba was supported by LLP Erasmus (2013/2014), Alexandru Ioan Cuza University, Iasi, Romani

Методология и клинические аспекты опухолей молочной железы у несовершенолетних

Simpozion Naţional de Oncologie cu participare internaţională „Vigilenţa oncologică în activitatea medicală, depistarea precoce şi tratamentul tumorilor” dedicat aniversării de 40 ani de activitate a Catedrei de Oncologie, Hematologie şi Radioterapie a USMF “N. Testemiţanu”Summary: This article highlights the importance of clinical and methodological aspects of the mammary gland tumors of the girls, and it is based on the study conducted between 2013-2015 in the University Clinic and Pediatric Oncology Department of the INSP IO. The stake of this study was to argue the frequency of the fibroadenomas and phylloid tumors.Резюме: В данной статье рассматриваются клинические и методологические аспекты oпухоли молочной железы у девочек, и оно основано на исследовании, проведенной между 2013-2015 в университетской клинике в отделении детской онкологии INSP IO. Цель данного исследования было подтвердить частоту фиброаденомы и филоидных опухолях

In Vitro Hypoxic Preconditioning of Bone Marrow Stromal Cells Triggers ERK-Mediated Signaling and Growth of L363 Myeloma Cells Celulele stromale ale măduvei hematopoietice precondiţionate la hipoxie in vitro induc proliferarea celulelor mielomatoase L363

Abstract Multiple myeloma (MM

SDS-PAGE ANALYSIS OF PROTEINS FROM THE SEEDS OF HONEYLOCUST (GLEDITSIA TRIACANTHOS) DURING GERMINATION

The main objective of the experiment was to observe the variety and evolution of the proteins during germination of the seeds from the honeylocust (Gleditsia triacanthos). Using the technique of SDS-PAGE there were discovered a lot of protein fractions. The profile of these fractions varies from the wide distribution along the entire molecular masses spectrum at the beginning of the germination to a poorly represented profile of protein fractions at the end of the germination

A preliminary analysis of the aspartateaminotransferase isozymes in Gleditsia triacanthos and Robinia pseudacacia germinating seeds

The extracts made with sodium/potassium phosphate buffer from the probes collected during germination of the seeds from Gleditsia triacanthos and Robinia pseudacacia was subjected to polyacrylamide gel electrophoresis under native conditions. Then the solution for specific coloring of the bands in which are found the izoenzymes of aspartate-aminotransferase was poured over the gel. The coloring reaction was stopped by rinsing the gel with distilled water when the intensity of the coloration was considered optimal. The results were interesting showing the existence of more than one spot corresponding to aspartate-aminotransferase in almost all analyzed probes. At least one of the spots from each of the probes presents the same Rf as the standard we have used (SigmaAldrich Glutamic- Oxalacetic Transaminase from porcine heart, Type II-A, lyophilized powder, 100-400 units/mg protein). The main purpose of the paper was to find if there are any different isozymes of the aspartate-aminotransferase in the extracts made with sodium/potassium phosphate buffer from the probes collected during germination of the seeds from Gleditsia triacanthos and Robinia pseudaca

THE IMPACT OF CoFeO 4 NANOPARTICLES ON SOLUBLE PROTEIN CONTENT AT WHITE ROT FUNGUS Phanerochaete chrysosporium

Experimental investigation focused on influence of different CoFeO4 nanoparticles concentrations on soluble protein and electrophoretic pattern of Phanerochaete chrysosporium fungus. Number of electrophoretic fraction is higher in older mycelium P. chrysosporium 14-day-old compared to that 7-day-old. Moreover, there is an increase in staining intensity of polypeptides of mycelium 14-day-old fact confirmed also by the higher amount of soluble protein. On the other hand, there are no visible differences as regards the different concentrations of nanoparticles effect on the polypeptides pattern

THE VALUES OF C - REACTIVE PROTEIN FOR SOME EARLY- DIAGNOSED WITH MYOCARDIAL INFARCTION PATIENTS

C-reactive protein (CRP) is an indirect risk factor in the acute coronary syndromes [2,13,21]. Its high levels may reflect one of the following cases: an inflammation of the coronary arteries as a response to the infectious agents; the severeness of the inflammatory response within the atherosclerotic vessels; inflammation expansion associated with myocardial ischemia; inflammation expansion associated with myocardial necrosis. Therefore, CRP indicates the cardiac lesions specific to various coronary heart diseases. The prognostic value of CRP augmentation appears to be more important in the patients affected by myocardial lesions [21]. This present study is aimed to determine the CRP levels in female and male patients of different age groups, early diagnosed with myocardial infarction

The influence of the attack of the fungus Melampsorella caryophyllacearum (DC.) J. Schrot. (Witch brooms on fir) on the peroxidase and catalase activity in host plant

n this paper are presented the results concerning the influence of the attack of the fungus Melampsorella caryophyllacearum (witch brooms on fir) on the peroxidase and catalase activity in host plant. The research were effectued in year 2007 in the stationary Izvorul Muntelui and Cerebuc from Ceahl�u massif and the obtained results highlights the fact that those two biochemical parameters were influenced differently by the attack of the parasitic fungus