DETERMINATION OF THE SPECIFIC FRAGMENT-IONS OF ERYTHROMYCIN AND SPIRAMYCIN BY PYROLYSIS MASS SPECTROSCOPY (PENENTUAN ION-ION FRAGMEN SPESIFIK ERITROMISIN DAN SPIRAMISIN DENGAN SPEKTROSKOPI MASSA PIROLISIS

ABSTRACT: Erythromycin and spiramycin were examined by pyrolysis mass spectrometer to determine the specific fragment-ions. It was found that fragment-ions at m/z 114, 115, 126, 127, 159, and 160 were the specific for erythromycin, whereas spiramycin has specific fragment-ions at m/z 142 and 158. Keywords: Erythromycin, spiramycin, pyrolysis mass spectrometry

STRUCTURAL REDETERMINATION OF NEW ERYTHROMYCIN DERIVATIVE : 2\u27,4"-O-DIMETHYLERYTHROMYCIN A USING 2D(I3C 1H)-NMR SPECTROMETRIC ANALYSIS (DETERMINASI KEMBALI STRUKTUR TURUNAN ERITROMISIN BARU: 2 \u27,4"-O-DIMETILERITROMISIN A

ABSTRACT Redetermination of the chemical structure of the sample E-1 has been carried out using 2D(\u27 3C-\u27H) NMR spectrometric approach. The appearance of cross-peaks at OHSc (3.2850.1) and (3.3350.0) strongly suggested the present of two methoxy groups at C-2\u27 of the desosarnine and C-4" of the cladinose respectively. This spectrometric analysis confirmed that the E-1 chemical structure is 2\u27.4"-O-Dimethylerythromycin A. Key-words : 2D(13C-\u27H) NMR. spectrometric approach

6,7.Anhldroerltromlsin.A Sebagai Metabolit Baru Dalam Fermentasi Saccharopolyspora ...

ABSTRACT Semisynthesisof A6.7-Anhydroerythromycin-Awas done by biomodification technique by addition of 0.2% INH into a culture fermentation of Saccharopolyspora erythraea ATCC 11635 in medium Hutchinson. The aim of this research is to studies of fragmentation pattern from new matabolite of A6.7-Anhydroerythromycin-Aby Liquid Chromatography-MassSpectroscopy(LC-MS) and the ionization of mass spectroscopy is use by ESI (Electrospray Ionization) pattern. The FT-IR spectrometric analyzes showed a stretching vibration of C=C conjugated group at wave number 1602.7 cm-1.This C=C conjugated vibrationindicated the existence of double bond between C6and C7 (A6.7),this confirmedthat isolate containedA6.7-Anhydroerythromycin(t-hAe possibilityof A6.7was positive).For complementation, a LC-MS (Liquid Chromatography-Mass Spectroscopy) analyzes using ESI-MS (Electrospray Ionization-Mass Spectroscopy) ionization pattern was conducted to the isolate which resulted Quassimolecularions [M+Hf of A7.8_and A6.7-AnhydroerythromycinL-CA-.MSspectrogramof the isolate,whichgave twopeaksof m/z 732.2460 and m/z 716.2522, confirmed that the m/z 732.2460possibly was A7.8-Anhydroerythromycin-A, while the m/z 716.2502andm/z 715.2522possibly were A6.7-Anhydroerythromycin-A. Keywords: isoniazid, enoyl reduction, A6,.7-Anhidroeritromisin-Afr, agmentation,LC-MS

Biosynthesis of A6\u277-anhydroerythromycin via enoyl reductase inhibition by isonicotinic hydrazide (INH)

Isonicotinic hydrazide (INH) was added to a fermentation of mutant of Saccharopolyspora erythraea ATCC 11912 to inhibit the activity of 2,4,6,8-tetramethyI-7,9-dihydroxy-2-en-5-on-undecyl-ACP enoyl reductase, an enzyme which catalyses enoyl reduction process of an intermediate in the erythronolide biosynthesis, and produce A"3-anhydroerythromycin derivative. The. optimum INH additional concentration of above 0.1% was able to inhibit the enzyme activity, and produce A6â¢7-anhydroerythromycin derivative as shown by FT-infrared spectrophotometric analysis of the product after its purification using thin layer chromatography. Keywords: Isonicotinic hydrazide (INH) enoyl reductase â d7-anhydroerythromycin â mutant Saccharopolyspor

Structural elucidation of A6\u277-anhydroerythromycin D using H-NMR-spectrometer

1-1-NMR-spectrometric analysis was carried out to samples I and 2 which were isolated respectively from Saccharopolyspora erythraea ATCC 11912 with and without additional isonicotinic hydrzide (INH). The sample I was confirmed as erythromycin D due to the proton appearance of 12-H at 32.3175 (dq) of the macrolactone, and the absence of 3"-niethoxy proton at 3.31 (s) of its neutral sugar. The sample 2 was confirmed as 46\u277-anhydroerythromycin D due to the presence of 7-H(-C=C-) at 35.343 (dq) instead of the methylene proton at C,and the presence of proton 12-Hat 82.318 (dq) of the macrolactone

Geometric Isomers and Cytotoxic Effect On T47D Cells of Curcumin Analogues PGV-0 and PGV-1

Curcumin analogues 2,5-bis-(4’-hidroxy-3’-methoxy)-benzilidinecylopentanone (PGV-0) and 2,5-bis-(4’hidroxy-3’,5’-dimethyl)-benzilidinecylopentanone (PGV-1) have a potency to be developed as cytotoxic agent. The aims of this research are to elucidate the geometric isomer and to study the cytotoxic effect on T47D cells of both compounds. To establish the geometric isomer these compounds, they were elucidated by LC-MS, 1H-NMR, 13C-NMR, HMBC, HMQC, NOESY. Their cytotoxic effect were evaluated by MTT assay method on T47D cells. The results concluded that the geometric isomer of PGV-1 is zusammen-zusammen (Z-Z) and PGV-0 is entgegen-entgegen (EE). The IC50 of both compounds are 1.74 and 9.39 μM respectively.Key words: PGV-0, PGV-1, Cytotoxicit

Biomimetic experiment of enoyl-reduction process by F420-dependent enzyme obtained from Saccharopolyspora erythraea and the biosynthetic implication

To approach the involvement of 5-deazaflavin coenzyme (F420) on the enoyl-reduction process in the step 4 of the erythronolide B in Saccharopolyspora erythraea, an investigation was carried out using biomimetic experiment. Three a,b¬unsaturated-bearing substances, i.e. ethyl crotonate, citral, and dimethyl glutaconate, were chosen as the hydride-acceptor substances in the experiment to mimic the step 4 intermediate: 7,9-dihydroxy-2,4,6,8-tetramethy1-5-oxo-undec-2-enoyl-S¬ACP. In the experiment crude extract isolated from Sac. erythraea was used to catalyze the hydride transfer from the NADPH, as the hydride donor, to the acceptors through F420. The hydride transfer was indicated by A401 of the coenzyme, before and after the transfer process which was under dim light and oxygen free condition. The results showed that coenzyme F420 has a role in the enoyl-reduction process of an a,b-unsaturated carbonyl structure. Keywords: Saccharopolyspora erythraea â F420 â enoyl-reduction â biomimetic experiment

Immobilization of DNA molecules on the metal surface through alkyl amine for biosensor applications

Efforts to immobilize DNA molecules directly onto the metal surface have been carried out by many researchers. At present, deposition of DNA onto the metal surface of gold is usually carried out through alkylthiol molecules. In this work, DNA molecules were succesfidly deposited on the metal surface of Indium Tin Oxide (ITO) through alkylamine of octadecylan tine (denoted as ODA). The optical properties characterization was carried out using LIV-Vis spectrometer for DNAs and DNAs mixed with Octadecylamine (denoted as DNAs-ODA) in liquid form. Film depositions of DNAs-ODA on the ITO substrate was also characterized. Based on the observation, it was found that the structure of metal-amine-DNA was potential to be used for DNA sensor applications.ln addition, electrical properties of ODA and DNA300-ODA were characterized by apply* various voltages to the sample and measuring its electrical current flow. It was found that the electrical resistance of ODA decreased due to the presence of the DNA molecules. Keywords: immobilization of DNA molecules âbiosensor application

APOPTOSIS INDUCTION EFFECT OF CURCUMIN AND ITS ANALOGS PENTAGAMAVUNON-0 AND PENTAGAMAVUNON-1 ON CANCER CELL LINES

ABSTRACTObjectives: This experiment aims to investigate the apoptosis effect of curcumin and its analogs pentagamavunon-0 (PGV-0) and PGV-1 on normaland other cancer cell lines.Methods: Growth inhibition effect was investigated using the MTT method. Double staining used acridine orange, 2-(4-aminodiphenyl)-6-indolcarbamidine dihydrochloride and ethidium bromide was performed to determine morphological changes of cells. Detection of PARP, caspase-3,PUMA and BAX using a western blot method was conducted to elucidate the apoptosis effect of the compounds.Results: PGV-1 (2.5 μM) and PGV-0 (5.0 μM) could inhibit T47D-cell growth on 72 h observation, but not for curcumin. DNA staining showed PGV-1has the strongest apoptosis induction effect on T47D-cells compared to PGV-0 and curcumin as well. Western blot analysis resulted in cleavage PARP(83 kD) on HeLa, T47D, and MCF-7 cells treated with PGV-1 (2.5 μM), PGV-0 (5.0 μM). Curcumin (10.0 μM) just induced apoptosis on T47D-cell andMCF-7 cell, but not HeLa cell. Cleavage PARP resulted by apoptosis process in the cell. PGV-1 (2.5 μM) had a stronger apoptosis effect compared toPGV-0 (5.0 μM) and curcumin (10.0 μM) based on cleaved PARP result qualitatively. On the normal cell (NH3T3), cells that were treated with thecompounds resulted in a negative cleavage PARP. This result indicated that the compounds were part of a selectively induced cancer cell line apoptosisprocess.Conclusion: Curcumin, PGV-0 and PGV-1 could inhibit cell growth by induce apoptosis on cancer cells but not on normal cells, which PGV-1 hasstrongest apoptosis induction effect on cancer cell lines.Keywords: Curcumin and analogs, Apoptosis, Cancer cell lines

The anticarcinogenic activity of plants compounds

The study was conducted to observe the effect of extracts of ngokilo (Gynura procumbens), beluntas (Pluchea indica), murbei (Morus alba) dan tapak doro (Vinca alba) leaves. Showed anticarcinogenic activity on lung tumor growth of mice. In the nex step, compounds having anticarcinogenic effect was isolated and identified, and evaluated on the cultures of meilomaand Vero cells. The results showed that non-polar fraction of ethanol extract of ngokilo leaves did not have anticarcinogenic activity, whereas the polar fraction show anticarcinogenic activity. At least there were three flavonoids (flavon or flavonol groups) in this polar fraction. It was only two of these flavonoids inhibit the growth of myeloma and Vero cells.Key words : ngokilo, Gynura procumbens, anticarcinogenic, flavonoid