Comparison of architect assay and elecsys HBs Ag II assay in the quantification of hepatitis B surface antigen in chronic HBV patients

Background. Hepatitis B virus (HBV) infection is a global health concern, with an estimated 296 million chronic carriers worldwide. Quantification of hepatitis B surface antigen (HBsAg) is a crucial tool in HBV disease management, providing valuable insights into disease progression and treatment efficacy. Objective. To evaluate the comparative performance of the Abbott Architect HBsAg QT and Roche Elecsys HBsAg II assays in quantifying hepatitis B surface antigen (HBsAg) levels in patients with chronic hepatitis B (CHB). Methods. This prospective study enrolled 60 confirmed CHB patients between February 2021 and November 2021. HBsAg levels were measured using both assays and subsequently log-transformed (log10 IU/mL) for statistical analysis. Interclass correlation coefficient (ICC), Pearson correlation coefficient, and Bland-Altman analyses were employed to assess assay concordance and systematic bias. Results. A statistically significant positive correlation was observed between the Elecsys HBsAg II and Architect HBsAg QT assays for both untreated and treatment-receiving CHB patients (ICC values not specified, p <0.001). Notably, Architect consistently yielded higher HBsAg measurements compared to Elecsys. Conclusion. Both the Elecsys HBsAg II and Architect HBsAg QT assays demonstrate robust correlation in quantifying HBsAg levels in CHB patients. However, the Architect assay exhibits a systematic positive bias relative to Elecsys, which should be considered when interpreting results and making clinical decisions. Both tools provide reliable assessments for HBsAg quantification within CHB management strategies, but it is essential to be aware of potential assay-specific differences

Surface treated fly ash filled modified epoxy composites

Abstract Fly ash, an inorganic alumino silicate has been used as filler in epoxy matrix, but it reduces the mechanical properties due to its poor dispersion and interfacial bonding with the epoxy matrix. To improve its interfacial bonding with epoxy matrix, surface treatment of fly ash was done using surfactant sodium lauryl sulfate and silane coupling agent glycidoxy propyl trimethoxy silane. An attempt is also made to reduce the particle size of fly ash using high pressure pulverizer. To improve fly ash dispersion in epoxy matrix, the epoxy was modified by mixing with amine containing liquid silicone rubber (ACS). The effect of surface treated fly ash with varying filler loadings from 10 to 40% weight on the mechanical, morphological and thermal properties of modified epoxy composites was investigated. The surface treated fly ash was characterized by particle size analyzer and FTIR spectra. Morphological studies of surface treated fly ash filled modified epoxy composites indicate good dispersion of fillers in the modified epoxy matrix and improves its mechanical properties. Impact strength of the surface treated fly ash filled modified epoxy composites show more improvement than unmodified composites

Serologic response to a secreted and a cytosolic antigen of Mycobacterium tuberculosis in childhood tuberculosis

Background and aim. Bacteriologic diagnosis of childhood tuberculosis is difficult, and alternate methods are needed. The utility of a serologic test for major secretory antigen (30 kDa) and a cytosolic antigen (16 kDa) of Mycobacterium tuberculosis was evaluated for the diagnosis of tuberculosis in children. Methods. Enzyme-linked immunosorbent assay was used. Specific IgG, IgA and IgM antibodies were measured in the sera from 26 clinically and/or bacteriologically diagnosed cases of childhood tuberculosis and 61 normal children. Results. Anti-IgG antibodies alone, against both 30- and 16-kDa antigens, were detected in 65.4% of patients. However, by combination of all three isotypes, increased sensitivities of 84.6 and 73%, with a specificity of 96.7% each, were obtained for 30- and 16-kDa antigens, respectively. Conclusions. We found good specificity and reasonably good sensitivity for detection of antibodies by enzyme-linked immunosorbent assay to 30-kDa antigen alone. The 16-kDa antigen did not perform as well

Purification, cloning, and DNA sequence analysis of a chitinase from an overproducing mutant of Streptomyces peucetius defective in daunorubicin biosynthesis

Extracellular chitinases of Streptomyces peucetius and a chitinase overproducing mutant, SPVI, were purified to homogeneity by ion exchange and gel filtration chromatography. The purified enzyme has a molecular mass of 42 kDa on SDS-PAGE, and the N-terminal amino acid sequence of the protein from the wild type showed homology to catalytic domains (Domain IV) of several other Streptomyces chitinases such as S. lividans 66, S. coelicolor A3(2), S. plicatus, and S. thermoviolaceus OPC-520. Purified SPVI chitinase cross-reacted to anti-chitinase antibodies of wild-type S. peucetius chitinase. A genomic library of SPVI constructed in E. coli using λ DASH II was probed with chiC of S. lividans 66 to screen for the chitinase gene. A 2.7 kb fragment containing the chitinase gene was subcloned from a λ DASH II clone, and sequenced. The deduced protein had a molecular mass of 68 kDa, and showed domain organization similar to that of S. lividans 66 chiC. The N-terminal amino acid sequence of the purified S. peucetius chitinase matched with the N-terminus of the catalytic domain, indicating the proteolytic processing of 68 kDa chitinase precursor protein to 42 kDa mature chitinase containing the catalytic domain only. A putative chiR sequence of a two-component regulatory system was found upstream of the chiC sequence

Integrated PV&ndash;BESS-Fed High Gain Converter for an LED Lighting System in a Commercial Building

The demand for electricity is rapidly growing and renewable energy sources such as solar, wind and tidal energy can compensate the demand to a substantial level. Among these, solar energy is abundant, scalable and is cheaper. The generated energy can be used in an efficient way if the DC output is directly supplied to the load instead of converting it to AC. Every electrical system is capable of operating in DC and, for example, energy efficient Light Emitting Diode (LED) lights have become popular as they provides more lumens with less power consumption and also can be directly operated from DC. LED lighting system in large commercial buildings has irradiance levels which vary sigificantly during operation. Extracting maximum power from the energy system and maintaining constant voltage output at different loads is another challenge. This paper proposes a solar Photo Voltaic (PV)-based energy system including Battery Energy Storage System (BESS) for supplying LED lamps to a commercial building through a modified high gain Luo converter. The Perturb and Observe control algorithm has been used for maximum power extraction from a PV cell whereas PI (Proportional Integral) controllers maintain constant output voltage from PV&ndash;BESS against different irradiance levels. To supply the desired voltages to the LED lighting system, a modified high gain Luo converter is designed. To make the output voltage constant at different load currents, PI and Sliding Mode Controllers (SMC) are designed with the help of the state-space average model. It is found that the sliding mode controller outperforms the PI controller in terms of behavior in the transient period and tracking capability. The system is simulated using MATLAB/Simulink&reg;. The Sliding Mode Controller has a 95% less transient period and is 75% faster in tracking capability when compared to other controllers. The system could be incorporated with the PV source to obtain green energy

Integrated PV–BESS-Fed High Gain Converter for an LED Lighting System in a Commercial Building

The demand for electricity is rapidly growing and renewable energy sources such as solar, wind and tidal energy can compensate the demand to a substantial level. Among these, solar energy is abundant, scalable and is cheaper. The generated energy can be used in an efficient way if the DC output is directly supplied to the load instead of converting it to AC. Every electrical system is capable of operating in DC and, for example, energy efficient Light Emitting Diode (LED) lights have become popular as they provides more lumens with less power consumption and also can be directly operated from DC. LED lighting system in large commercial buildings has irradiance levels which vary sigificantly during operation. Extracting maximum power from the energy system and maintaining constant voltage output at different loads is another challenge. This paper proposes a solar Photo Voltaic (PV)-based energy system including Battery Energy Storage System (BESS) for supplying LED lamps to a commercial building through a modified high gain Luo converter. The Perturb and Observe control algorithm has been used for maximum power extraction from a PV cell whereas PI (Proportional Integral) controllers maintain constant output voltage from PV–BESS against different irradiance levels. To supply the desired voltages to the LED lighting system, a modified high gain Luo converter is designed. To make the output voltage constant at different load currents, PI and Sliding Mode Controllers (SMC) are designed with the help of the state-space average model. It is found that the sliding mode controller outperforms the PI controller in terms of behavior in the transient period and tracking capability. The system is simulated using MATLAB/Simulink®. The Sliding Mode Controller has a 95% less transient period and is 75% faster in tracking capability when compared to other controllers. The system could be incorporated with the PV source to obtain green energy

Integrated PV–BESS-Fed High Gain Converter for an LED Lighting System in a Commercial Building

The demand for electricity is rapidly growing and renewable energy sources such as solar, wind and tidal energy can compensate the demand to a substantial level. Among these, solar energy is abundant, scalable and is cheaper. The generated energy can be used in an efficient way if the DC output is directly supplied to the load instead of converting it to AC. Every electrical system is capable of operating in DC and, for example, energy efficient Light Emitting Diode (LED) lights have become popular as they provides more lumens with less power consumption and also can be directly operated from DC. LED lighting system in large commercial buildings has irradiance levels which vary sigificantly during operation. Extracting maximum power from the energy system and maintaining constant voltage output at different loads is another challenge. This paper proposes a solar Photo Voltaic (PV)-based energy system including Battery Energy Storage System (BESS) for supplying LED lamps to a commercial building through a modified high gain Luo converter. The Perturb and Observe control algorithm has been used for maximum power extraction from a PV cell whereas PI (Proportional Integral) controllers maintain constant output voltage from PV–BESS against different irradiance levels. To supply the desired voltages to the LED lighting system, a modified high gain Luo converter is designed. To make the output voltage constant at different load currents, PI and Sliding Mode Controllers (SMC) are designed with the help of the state-space average model. It is found that the sliding mode controller outperforms the PI controller in terms of behavior in the transient period and tracking capability. The system is simulated using MATLAB/Simulink®. The Sliding Mode Controller has a 95% less transient period and is 75% faster in tracking capability when compared to other controllers. The system could be incorporated with the PV source to obtain green energy