Vermicompost effect on the trace elements distribution in the luvic chernozem of the Krasnoyarsk forest-steppe

n the field experiment (2016-2018) in the conditions of the Krasnoyarsk forest-steppe the number of trace elements mobile forms were determined and the effect of soil organic matter and increasing doses of vermicompost on their distribution in luvic chernozem was studied. It is established that the studied vermicompost does not pollute the soil, which allows its widespread use in agriculture. The correlation and regression analysis obtained linear regression equations that reflect the relationship of the chemical element and organic matter in the soil after using increasing doses of vermicompost adequately. Elements with positive correlation and high significance level include Mn, Cu, Co and Zn

Usage of Colors in the Corporate Company’ s Styl (on the Example of Tiffany & Co)

В данной статье рассмотрен фирменный цвет бренда Tiffany & Co: от истории создания до сегодняшних дней. Даётся толкование смешению цветов и их влиянию на целевую аудиторию. Каждый цвет в статье рассмотрен отдельно для более ясного понимания его влияния на человека.This article describes the corporate color of the brand Tiffany & Co: from the history of creation to the present day. An interpretation is given of color mixing and their influence on the target audience. Each color in the article is reviewed separately for a clearer understanding of the effect on a person

The diagram development for Computer Added Control and Monitoring system of drilling

The paper is concerned with the first stage of the extensive research aimed at developing design-automation system and well drilling process control. The proposed system is going to have some advantages over modern analogues, such as economic analysis at all levels, active engineering staff feedback, precedent-related principle for recommendations, etc. It will essentially reduce the risk of human errors and also optimize the well construction process from design to commissioning. The paper considers the results of the first design stage in a form of flow diagrams

Development of engineering solutions for air drilling at Dulisminsk oilfield, Irkutsk oblast

The article presents the analysis of disastrous mud loss achieved during casing at Dulisminsk oil and gas condensate field (DOGCF) and comparative evaluation of geological structure of DOGCF and Naryksko-Ostashkinsk area of Kemerovo oblast, where the same problem was solved successfully. On the basis of the analysis, an engineering solution is proposed to prevent mud loss in the conditions of DOGCF

Improving Bacillus Altitudinis B-388 Genome Scaffolding Using Mate-Pair Next-Generation Sequencing

© 2016, Springer Science+Business Media New York.Bacillus species, generally regarded as soil microorganisms, are present in human gastrointestinal tract (GIT) in quantities, which cannot be explained by their entrance with food only. They are capable of growing in GIT and interacting with intestinal microbiota and host organism by excretion of enzymes and low-molecular weight compounds, which exert digestion-facilitating, antagonistic, immunomodulating, antiviral, anticancer properties or mediate cell communication. For better understanding of its probiotic potential, we have sequenced genome of Bacillus altitudinis B-388 using mate-pair technology. It allowed us to improve quality of the genome sequence. The number of contigs decreased from 59 to 8. N50 contig length increased by four times. The number of identified genes raised from 3730 to 3774 (3645 proteins and 73 RNAs) with the reduction of frameshifted genes. The calculated size of B. altitudinis B-388 genome is 3,743,699 bp, with a G + C content of 41.17 mol%. Additional incomplete prophage sequence in genome of B. altitudinis B-388 was revealed. It was found that cryptic plasmid encodes SoxR, an oxidative stress response regulator. To date, the reported sequence is the most thorough presentation of B. altitudinis genome among four whole-genome sequences of this species deposited in GenBank

Project-based method in teaching foreign language for specific purposes

This paper considers the innovative methods of teaching Foreign Language for Specific Purposes. The methods are intensively implemented in teaching 3–4-year students at the Institute of Natural Resources, Tomsk Polytechnic University (TPU), Russia. In particular, the paper presents the international project “Approaching the Arctic” developed in cooperation with NTNU (Norwegian University of Science and Technology), Norway. This paper analyzes the efficiency of the implementation of the project-based method in teaching English for Specific Purposes

Visible light photoluminescence in TiO2/CdS nanopowders synthesized by sol-gel route: Effect of gel aging time

A series of sol-gel TiO2/CdS, TiO2 powders and coagulated CdS nanoparticles were studied by XRD, HRTEM and Raman spectroscopy to elucidate the effect of low-temperature gel aging time on visible photoluminescence (PL) emission of the TiO2/CdS composites. With an increase in aging time a content of amorphous titania and incorporated CdS nanoparticles decreases in composites. For all composites, visible PL emission includes bands attributed to surface oxygen vacancies and hydroxyl group of TiO2 nanocrystals, as well as yellow-green and red bands related to lattice defect states of CdS nanoparticles. It was found that gel aging time is a crucial parameter to influence visible PL emission in composites. This emission is suppressed with increasing aging time, and its evolution shows that healing of oxygen vacancy defects and hydroxyl group affect visible emission more significantly than improving crystallinity degree. The correlation between visible PL emission in TiO2/CdS and their visible-light photocatalytic activity was discussed. © 2020, ITMO University. All rights reserved.The research was carried out in accordance with the state assignment for ISSC UB RAS and financial support from ISSC UB RAS (theme AAAA-A19-119031890025-9). TEM study was supported by RFBR (grant No. 20-02-00906). The authors are grateful to Dr. Kozhevnikova N. S. and Dr. Gorbunova T. I. for the provided samples TiO2/CdS and CdS

Species identification of spider mites (Tetranychidae: Tetranychinae): a review of methods

Spider mites (Acari: Tetranychidae) are dangerous pests of agricultural and ornamental crops, the most economically significant of them belonging to the genera Tetranychus, Eutetranychus, Oligonychus and Panonychus. The expansion of the distribution areas, the increased harmfulness and dangerous status of certain species in the family Tetranychidae and their invasion of new regions pose a serious threat to the phytosanitary status of agroand biocenoses. Various approaches to acarofauna species diagnosis determine a rather diverse range of currently existing methods generally described in this review. Identification of spider mites by morphological traits, which is currently considered the main method, is complicated due to the complexity of preparing biomaterials for diagnosis and a limited number of diagnostic signs. In this regard, biochemical and molecular genetic methods such as allozyme analysis, DNA barcoding, restriction fragment length polymorphism (PCR-RFLP), selection of species-specific primers and real-time PCR are becoming important. In the review, close attention is paid to the successful use of these methods for species discrimination in the mites of the subfamily Tetranychinae. For some species, e. g., the two-spotted spider mite (Tetranychus urticae), a range of identification methods has been developed – from allozyme analysis to loop isothermal amplification (LAMP), while for many other species a much smaller variety of approaches is available. The greatest accuracy in the identification of spider mites can be achieved using a combination of several methods, e. g., examination of morphological features and one of the molecular approaches (DNA barcoding, PCR-RFLP, etc.). This review may be useful to specialists who are in search of an effective system for spider mite species identification as well as when developing new test systems relevant to specific plant crops or a specific region

The choice of antibiotic for community-acquired pneumonia – the results of a survey of doctors and an analysis of real outpatient practice

Community-acquired pneumonia (CAP) remains an urgent problem in the structure of infectious diseases worldwide including the Russian Federation. According to medical statistics the incidence rate of CAP increased by 19.1 % by December 2018 as compared to year 2017. The authors addressed the problem of rational choice of antimicrobial agents (AMAs) for outpatient treatment of this disease. The aim was to assess structure and rationality of choice of AMAs for outpatient treatment of CAP by primary care physicians in municipal outpatient clinics in Moscow. A two-stage single-center pharmacoepidemic study was conducted. The survey involved 45 primary care physicians with average age 48.5 ± 13.2 years and medical experience 22.7 ± 13.6 years. Relevant information was also copied out from 650 ambulatory records of patients receiving AMs for CAP. According to the results of our survey the leaders of the respondents’ preferences are beta-lactam antibiotics — amoxicillin/clavulanate (49.0 %) followed by amoxicillin (25.5 %) and cephalosporins (15.7 %). Analysis of real clinical practice brings different picture: cephalosporins become comparable to amoxicillin/clavulanate in terms of frequency of administration, The latter significantly loses popularity, as does amoxicillin as monotherapy These data match with similar survey of 233 Moscow primary care physicians conducted earlier in 2013-2014 by one of the authors where amoxicillin/clavulanate accounted for 43.6 % of total AMAs recommendations. Thus, the initial choice of AMAs for outpatient treatment of CAP in most cases does not comply with current clinical guidelines. Physicians tend to start initial outpatient management of CAP with second-line AMAs (amoxicillin/clavulanate, cephalosporins, less commonly fluoroquinolones), underestimating macrolides and almost neglecting doxycycline. These circumstances result in unwarranted increase in drug load on the patient, decrease in safety of treatment and ineffectiveness of antimicrobial therapy in case if CAP is caused by atypical pathogens

A Novel Antiviral Strategy against MERS-CoV and HCoV-229E Using Binase to Target Viral Genome Replication

© 2016, Springer Science+Business Media New York.RNA viruses cause most of the dangerous communicable diseases. Due to their high mutation rates, RNA viruses quickly evade selective pressures and can adapt to a new host. Therefore, new antiviral approaches are urgently needed, which target more than one specific virus variant and which would optimally prevent development of viral resistance. Among the family of coronaviruses (CoV), several human pathogenic strains (HCoV) are known to cause respiratory diseases and are implied in enteric diseases. While most strains contribute to common cold-like illnesses, others lead to severe infections. One of these viruses is the newly emerged (2012), highly pathogenic Middle East respiratory syndrome coronavirus (MERS-CoV) of zoonotic origin. MERS-CoV causes a severe respiratory infection with a high mortality rate of 35 %. There is no specific treatment or infection prevention available. Here, we show that the bacterial ribonuclease Binase is able to inhibit the replication of MERS-CoV and of the low-pathogenic human coronavirus 229E (HCoV-229E) in cell culture. We demonstrate that at non-toxic concentrations, Binase decreased the titers of MERS-CoV and HCoV-229E. On a molecular level, Binase treatment reduced (i) the viral subgenomic RNAs and (ii) the viral nucleocapsidprotein (N) and non-structural protein 13 (nsp13) accumulation. Furthermore, we show that the quantity of the replication/transcription complexes within the infected cells is diminished. Thus, the data obtained might allow further development of new anti-coronaviral approaches affecting viral replication, independent of the specific virus strain