Immunization of Rainbow Trout (Oncorhynchus mykiss) against Lactococcus garvieae Using Vaccine Mixtures

The effectiveness of vaccine mixtures against lactococcosis was tested in rainbow trout (Oncorhynchus mykiss). The M1 strain of Lactococcus garvieae, isolated from a recent outbreak of lactococcosis at a rainbow trout farm in Turkey, was used in a trial comparing five immuniza- tion treatments: (a) formalin inactivated bacterin (vaccine), (b) the above bacterin together with Freund’s Incomplete Adjuvant (FIA), (c) the bacterin combined with β-glucan, (d) β-glucan only, and (e) phosphate buffered saline-PBS (control). Fish were given intrapritoneal injections and challenged by exposure to the bacteria 30, 75, or 125 days after vaccination. In fish exposed to the bacteria 30 days after injection, the relative percent survival (RPS) was 88.89% in the group that received only bacterin and 100% in the group that received the bacterin combined with FIA. Immunity remained high in the bacterin+FIA group, as the RPS in this group remained 100% in fish challenged at 75 days, significantly higher than in all other groups. In fish exposed to the bacteria 125 days after vaccination, the RPS was 54.55% in fish vaccinated with the bacterin only and 84.84% in fish vaccinated with bacterin+FIA. In the group that received only β-glucan, immunity did not improve after vaccination. Micro-agglutination tests of serums showed that immunized fish produced antibodies at high titers within 30 days. In short, the formalin-inacti- vated M1 strain provided longer lasting protection against Lactococcus garvieae in rainbow trout when combined with FIA than when administered alone or with β-glucan

functionally graded material under wet and dry sliding conditions

This study investigated that the effect of aging treatments on wear behavior of functionally graded material (FGM) that was reinforced via being integrated with aluminum 2014 alloy (AlCu4SiMg) and 15 vol% SiC. The specimens were obtained via centrifugal casting technique, and then two different aging treatments were applied. Wear experiments were applied at 1.256 m/s constant sliding velocity, under two different loads and two different sliding distances for each condition via a pin-on-disc wear apparatus. The variations that occurred on wear behavior of cast and aged materials were analyzed. The results show that the minimum wear loss values were obtained under dry sliding conditions due to the aging processes. On the other hand, with increasing sliding distances under wet sliding conditions, the aging processes' effect was decreased on wear resistance

steel

In the present study, gears made of 21NiCrMo2 (AISI 8620) steel were subjected to heat treatments including carburizing, decarburizing, boriding and hardening. Carburizing, decarburizing and boriding were performed in a gaseous atmosphere, a salt bath consisting of 60% NaCl and 40% NaCO3, and a liquid medium containing borax and silicon carbide, respectively. Borocarburazing was carried out in two steps: carburizing and then boriding. Some specimens were treated to obtain different carbon concentrations and to observe the effect of carbon content on the boriding process. This process, consisting of carburizing followed by decarburizing and finally boriding, is named borodecarburizing. The microstructures and phase compositions of the diffusion layers were examined by means of X-ray diffractometry, scanning electron microscopy and optical microscopy. The microhardness profiles of these layers were studied by a Vickers indenter. The hardness value obtained by the borodecarburizing process is 10% higher than a borocarburized specimen's hardness. One-phase iron boride zone (Fe2B) was observed in the layers. This phase is preferred due to its mechanical properties