Molecular Typing of Turkish Apple Chlorotic Leaf Spot Virus Isolates Based on Partial Coat Protein Gene

Apple chlorotic leaf spot virus (ACLSV) isolates from various hosts and geographic locations in Turkey were molecularly characterized by RFLP, nucleotide sequence analysis and the construction of a phylogenetic tree including ACLSV isolates from GenBank. Based on nucleotide sequence alignment and the phylogenetic tree, we proposed a classification of ACLSV isolates in which isolates were divided into three major groups. The first group contained mainly Far-Eastern isolates, the second group the Hungarian (eastern-European) ACLSV isolates, and the third group, which contained isolates of variable characteristics, was again divided into two subgroups, subgroup I containing mixed European isolates, and subgroup II containing central European isolates. Three representative Turkish ACLSV isolates belonged to the third group; of these, one was from the mixed European cluster (subgroup I) and two from the central European cluster (subgroup II). The nucleotide sequence divergence and geographic origin of the ACLSV isolates were correlated, which indicated the possible extraction of the Turkish isolates

Comparison of Serological and Molecular Detection Methods for Testing Individual and Composite Samples Using PPV-M and PPV-T Isolates

2nd International Symposium on Plum Pox Virus -- SEP 03-06, 2013 -- Olomouc, CZECH REPUBLICWOS: 000358036400024Sharka disease of stone fruit trees caused by Plum pox virus (PPV) was first described in 1968 in a limited area of Turkey, but during the last decade the disease has progressively spread to a large part of the country. Although PPV-Rec and -D strains were found in Turkey, the most common PPV strains were detected as PPV-M and PPV-T. In this study, DAS-ELISA (5B-IVIA/AMR) monoclonal antibody) and Spot Real-time RT-PCR techniques have been evaluated in order to determine the best sampling time and ratio of PPV infected samples in non-infected-infected plant mixtures for detection of PPV-T and PPV-M strains. Dormant buds in winter and fresh leaves in spring from PPV-infected trees were used for testing in 2012. Six repetitions were performed by single (3 leaves or buds from infected plant) or composite plant mixture samples (3 leaves or buds from infected plant + 3 leaves from healthy plant, and the other composite samples, i.e., 3+6 to 3+27). All combinations and all repetitions of composite leaf samples of both strains were detected as positive in Spot Real-time RT-PCR. However, in DAS-ELISA, the number of PPV positive samples decreased for T and M strain in 6th composite (3 infected+12 healthy leaves) and in 9th composite (3 infected+21 healthy leaves) in spring, respectively. At least 3 repetitions in all combinations of composite samples for PPV-T and -M were found positive in dormant season by Spot Real-time RTPCR whereas it was negative only in the last composite sample (3 infected+27 healthy buds) of PPV-T by DAS-ELISA.Int Soc Horticultural Sc

Fourthy-Five Years of Sharka Disease in Turkey

2nd International Symposium on Plum Pox Virus -- SEP 03-06, 2013 -- Olomouc, CZECH REPUBLICWOS: 000358036400004Sharka disease in Turkey has firstly been reported in 1968 in Edirne (Marmara region) which is located next to the Bulgarian border. Nowadays, new PPV outbreaks have been reported in Central Anatolia (Ankara, Kayseri), Aegean (Izmir) and Mediterranean regions (Adana, Mersin, Hatay). The distribution of PPV strains was mainly related to the geographical location and the period of PPV introduction in these regions. PPV-M was mainly detected in peach, nectarine and apricot which were recently imported from abroad to the Mediterranean region. PPV-T was detected in apricot and plums in Central Anatolia and in the Aegean Regions where PPV has been endemic and existing for years. These distributions might indicate that new outbreaks may be mainly due to latently infected material that has passed through the border control. Epidemiology and rootstock susceptibility to PPV has also been recently accomplished. A breeding program has been started in 2006 and its main aim is to obtain dried apricot cultivars resistant to PPV and well adapted to Turkish conditions.Int Soc Horticultural Sc

First Report of Phytophthora cinnamomi Causing Root and Crown Rot of Ficus carica in Turkey

WOS: 000462807700057

First Report of Phytophthora chlamydospora Causing Root Rot on Walnut (Juglans regia) Trees in Turkey.

WOS: 000385608800065

Experimental transmission efficiency of Plum pox virus T by Myzus persicae Sulzer (Homoptera: Aphididae)

3rd International Symposium on Plum Pox Virus -- MAY 09-13, 2016 -- Antalya, TURKEYWOS: 000428232700014…Int Soc Horticultural SciResearch Fund of Mustafa Kemal University [BAP-348]This research was supported by the Research Fund of Mustafa Kemal University (project number BAP-348). We are really thankful to the Quarantine Directorate of Izmir for technical support and Mr. Mahmood Ayyaz from Nigde University for helping with technical writing and improving the English of the manuscript

First Report of Neoscytalidium dimidiatum Causing Canker, Shoot Blight, and Root Rot of Pistachio in Turkey

WOS: 00046885630005

Characterization of bacterial knot disease caused by Pseudomonas savastanoi pv. savastanoi on pomegranate (Punica granatum L.) trees: a new host of the pathogen

WOS: 000343871400010PubMed ID: 25039423This study aimed to isolate and identify the causal organism causing hyperplastic outgrowths (knots) on stems and branches of pomegranate trees in the Eastern Mediterranean region of Turkey. Bacterial colonies were isolated from young knots on plates containing selective nutrient media. Biochemical tests, fatty acid analysis and PCR were performed to identify possible causal disease agent. Representative isolates were identified as Pseudomonas.pv.savastanoi (Psv) using biochemical tests, fatty acid profiling and PCR. Following inoculation of pomegranate plants (cv. hicaz) with bacterial suspensions, 25 of 54 bacterial isolates caused typical knots at the site of inoculation. PCR analysis, using specific primer for Psv, generated a single amplicon from all isolates. The similarity of the sequence of Turkish pomegranate isolate was 99% similar to the corresponding gene sequences of Psv in the databases. Based on symptoms, biochemical, molecular, pathogenicity tests and sequence analyses, the disease agent of knots observed on the pomegranate trees is Psv. To the best of our knowledge, this research has revealed pomegranate as a natural host of Psv, which extends the list of host plant species affected by the pathogen in the world and Turkey. Significance and Impact of the StudyPomegranate trees were affected by the disease with outgrowths (galls or knot) disease. Currently, there is no published study on disease agent(s) causing the galls or knots on pomegranate trees in worldwide. Bacterial colonies were isolated from young knots. The causal agent of the knot Pseudomonas savastanoi pv.savastanoi (Psv) was identified based on symptoms, biochemical, molecular methods, pathogenicity tests and sequence analysis. To the best of our knowledge, this is the first report of Psv on pomegranate as a natural host, which extends the growing list of plant species affected by this bacterium in the world and Turkey

Screening for Resistance to Plum Pox Virus in Some Local Turkish Apricot Cultivars and Their Crosses by Molecular Markers

2nd International Symposium on Plum Pox Virus -- SEP 03-06, 2013 -- Olomouc, CZECH REPUBLICWOS: 000358036400017Turkey is the most important producer and exporter country of apricot, Prunus armeniaca. Production of apricots for fresh market relies on foreign cultivars grown on Mediterranean and Aegean regions while Malatya is the most important region for production of dry apricots based on local cultivars. Plum pox virus (PPV) in Turkey has been known since 1968, but it was not widespread until recent years. Malatya region has been free of sharka disease so far, but the disease has already been reported from many different provinces since 2006. Because of that, introgression of resistance to PPV in the local cultivars with good pomological characteristics became an important objective for the apricot crop. In the current breeding program, obtaining new cultivars resistant to PPV, selection of resistant seedlings by using molecular markers linked to PPV resistance was aimed at. Nineteen local apricot genitors and progenies obtained from the crosses between the PPV resistant cultivar 'Stark Early Orange' (SEO), 'Harcot' and local cultivars such as 'Hacihaliloglu', 'Kabaasi', 'Hasanbey', 'Cologlu', 'Adilcevaz5', 'Sekerpare', 'MahmudunErigi', 'Soganci' and 'Cataloglu' were screened with markers. The markers PGS1.21 and PGS2.23 co-segregating with resistance to PPV were used to screen a total of 189 apricot progenies. None of the local genitors had alleles linked to PPV resistance. Among the progenies screened, 15 seedlings from 'Sekerpare' by SEO, 12 from 'Adilcevaz5' by SEO, 7 from 'Hacihaliloglu' by SEO, 9 from 'Kabaasi' by SEO, 5 from 'Cologlu' by SEO, 9 from 'Cataloglu' by SEO, 4 from 'Hasanbey' by SEO, and 1 from 'MahmudunErigi' by SEO and none of the 'Harcot' by 'Soganci' presented resistant alleles and were selected for further studies.Int Soc Horticultural Sc

Investigation of resistance of apricot progeny to Plum pox virus through molecular markers

3rd International Symposium on Plum Pox Virus -- MAY 09-13, 2016 -- Antalya, TURKEYWOS: 000428232700005Plum pox virus (PPV) is the causal agent of sharka disease, which is mainly destructive on apricot (Prunus armeniaca L.), plum (Prunus domestica L.) and peach (Prunus persica L.). There are no efficient control methods except using PPV-free propagating materials and planting PPV-resistant or at least less-susceptible rootstocks. Therefore, lots of studies have been conducted in recent years on breeding of PPV-resistant plants. The objective of this study was the introduction and development of marker-assisted selection (MAS) for PPV resistance in F-1 and F-2 progenies of some Turkish apricot cultivars. Local apricot cultivars 'Adilcevaz 5', 'cologlu', 'Sekerpare' and 'cataloglul crossed with PPV-resistant 'Stark Early Orange' (SEO) were screened with molecular markers PGS1.21 and PGS2.23 co segregating with resistance to PPV in 2011. Of all combinations, seven of 20 progeny obtained from SEO x 12 of 34 progeny obtained from SEO x 'Adilcevaz 5, five of 10 progeny obtained from SEO x 'coloklu, 15 of 37 progeny obtained from SEO x `Sekerpare' and eight of 33 progeny obtained from SEO x 'cataloglu' exhibited resistant alleles.Int Soc Horticultural Sc