In Vivo Consumption of Cranberry Exerts ex Vivo Antiadhesive Activity against <i>FimH</i>-Dominated Uropathogenic Escherichia coli: A Combined in Vivo, ex Vivo, and in Vitro Study of an Extract from Vaccinium macrocarpon

For investigation of the molecular interaction of cranberry extract with adhesins of uropathogenic Escherichia coli (UPEC), urine from four volunteers consuming standardized cranberry extract (proanthocyanidin content = 1.24%) was analyzed within ex vivo experiments, indicating time-dependent significant inhibition of 40–50% of bacterial adhesion of UPEC strain NU14 to human T24 bladder cells. Under in vitro conditions a dose-dependent increase in bacterial adhesion was observed with proanthocyanidin-enriched cranberry Vaccinium macrocarpon extract (proanthocyanidin content = 21%). Confocal laser scanning microscopy and scanning electron microscopy proved that <i>V.m.</i> extract led to the formation of bacterial clusters on the outer plasma membrane of the host cells without subsequent internalization. This agglomerating activity was not observed when a PAC-depleted extract (<i>V.m.</i> extract^≠PAC) was used, which showed significant inhibition of bacterial adhesion in cases where type 1 fimbriae dominated and mannose-sensitive UPEC strain NU14 was used. <i>V.m.</i> extract^≠PAC had no inhibitory activity against P- and F1C-fimbriae dominated strain 2980. Quantitative gene expression analysis indicated that PAC-containing as well as PAC-depleted cranberry extracts increased the <i>fimH</i> expression in NU14 as part of a feedback mechanism after blocking <i>FimH</i>. For strain 2980 the PAC-containing extract led to up-regulation of P- and F1C-fimbriae, whereas the PAC-depleted extract had no influence on gene expression. <i>V.m.</i> and <i>V.m.</i> extract^≠PAC did not influence biofilm and curli formation in UPEC strains NU14 and 2980. These data lead to the conclusion that also proanthocyanidin-free cranberry extracts exert antiadhesive activity by interaction with mannose-sensitive type 1 fimbriae of UPEC

Analysis and Desing of Information System

Import 05/08/2014Předložená bakalářská práce se zabývá analýzou a návrhem informační podpory pro správu vinohradů. Zadání pro bakalářskou práci definovala firma FULLCOM systems s.r.o., která se zabývá vývojem ERP systémů zejména pro potravinářský průmysl s cílem analyzovat a vytvořit rozšíření funkcionality jejich ERP systému FIS. V práci je uvedena vstupní analýza požadavků na systém, na základě které byl vypracován návrh databázové struktury a řešení informační podpory pro správu vinohradu včetně propojení s ERP systémem FIS.The bachelor thesis deals with analysis and design of information support for vineyard management. Task was defined by FULLCOM systems s.r.o., a company which main activity is area of ERP systems for food industry. The aim of the thesis is analyse and develop the extension of ERP system FIS for vineyard management. The thesis contains design of database structure and solution of information support based on requirements analysis. Thesis also includes solution of connection of designed system to ERP FIS.Prezenční545 - Institut ekonomiky a systémů řízenívelmi dobř

Additional file 3: Figure S1. of No evidence for a bovine mastitis Escherichia coli pathotype

Minimum spanning tree (MST) of the MLST results. Figure S2. Phylograms and tanglegrams for the 25 bovine-associated E. coli genomes based on WGA core genome, gene and VF content. Figure S3. Manhattan plots of Fisher’s exact test p-values for the OG/VF pathotype (MAEC/commensal isolates) and phylogroup (A/B1) associations. Figure S4. Gene organization of the AMR-SSuT/Tn10 gene cluster. Figure S5. Circular genome diagrams for the MAEC 1303 and ECC-1470 replicons with GI and prophage positions. Figure S6. Heatmap of VF presence/absence, including gene names/locus tags. Figure S7. Gene organization of the Flag-2 and ECC-1470 T6SS/1 gene clusters. (PDF 18 Mb

Content Management System in SMB

Import 05/08/2014Tato bakalářská práce se zabývá analýzou možností tvorby efektivní webové prezentace za použití redakčního systému. První část práce se věnuje firmě. Je zde nastíněn popis a organizace firmy a její informační toky. Dále jsou jmenovány požadavky na funkce redakčního systému. Druhá část práce se zabývá redakčními systémy. V teoretické části jsou vysvětleny základní funkce a principy redakčních systémů. Praktická část porovnává, nakolik funkce jednotlivých vybraných redakčních systémů splňují požadavky firmy. Nakonec je na základě zjištěných poznatků navrženo vhodné typové řešení pro danou firmu.This bachelor thesis is dedicated to analysis how to create an effective web presentation by means of content management system. The first part of the thesis is dedicated to a company. It contains description and organization of a company and its information flows. Furthermore, requirements for content management functions are stated. The second part of the thesis is dedicated to content management systems. In the theoretical part, basic functions and principles of content management systems are explained. In the practical part, it is compared to which extend functions of selected content management systems fulfill requirements of a company. In the end, a suitable type of solution is suggested for the given company based on gathered findings.Prezenční545 - Institut ekonomiky a systémů řízenívýborn

Creating and Comparing of Making Survey Sketch in Czech Republic and Slovakia

Import 05/08/2014Tato diplomová práce se zabývá vyhotovením geometrických plánů v České a Slovenské republice a jejich vzájemným porovnáním. Porovnává vyhotovení geometrických plánů z pohledu jejich tvorby, právních předpisů, formálních náležitostí, obsahu a dalších důležitých kritérií. Aby bylo možné geometrické plány vytvořit a porovnat, bylo nutné důkladně se seznámit s legislativou v obou zemích. V diplomové práci byly použity citace ze zákonů, vyhlášek a metodických pokynů jednotlivých zemí a příslušných katastrech nemovitostí. Práce se zároveň v jejím úvodu zabývá historickým vývojem katastru nemovitostí na územích České a Slovenské republiky obecně, jakož i konkrétní vývojem samotných geometrických plánů. Podrobně popisuje a porovnává jejich grafické a písemné části a zbylou dokumentaci. Obsahuje informace o aktuálních legislativních předpisech, které upravují tvorbu a obsah geometrických plánů na Slovensku a v České republice.This Master Thesis delves into the complex process of geometrical survey and map production in Slovakia and in the Czech Republic and compares geometrical survey processes in the two countries. This work assesses the processes involved in geometrical surveys in Slovakia and in the Czech Republic and compares them based on crucial criteria including survey concept and creation, legal requirements and other formalities. In order to realize such a comprehensive Master Thesis, it was necessary to familiarize oneself fully with the researched topic through extensive study of legislature, Land Registry guidelines and regulations and methodical directives of both countries in question. In its Intro, this Master Thesis elaborates on the history of Land Registries in Slovakia and in the Czech Republic, as well as on the history of geometrical maps in both countries. The author examines the graphic and the written part of geometrical maps in great detail and cross-examines further supporting documentation. This Master Thesis also includes contemporary legislative directives which govern the production and the content of geometrical maps in Slovakia and in the Czech Republic.Prezenční544 - Institut geodézie a důlního měřictvívýborn

Additional file 2: Table S2. of No evidence for a bovine mastitis Escherichia coli pathotype

MLST allele profiles, ST and CC numbers for the 64 E. coli and four Shigella spp. strains. (XLSX 10 kb

Additional file 5: Table S3. of No evidence for a bovine mastitis Escherichia coli pathotype

This file includes the SRA study accession numbers for the Illumina and 454 raw reads of the 14 E. coli genomes of this study. Additionally it lists the assembly statistics for all 23 bovine-associated E. coli draft genomes. (XLSX 8 kb

Regulatory network of the Csr system.

<p>Lines with an arrow denote positive control, and lines with a bar represent negative control.</p

Phenotypic characterization of Nissle 1917 and its isogenic Csr mutants.

<p>Each experiment was performed in three independent biological replicates. The data shown here are semi-quantitative.</p

<i>E</i>. <i>coli</i> AfaE-III suppresses epithelial exfoliation in CEAtg mice.

<p><i>(A)</i> Whole mount urogenital tracts of uninfected wild-type or CEAtg female mice were fixed and processed for scanning electron microscopy (SEM). Pictures show the luminal surface of the upper vaginal and cervical regions and are representative for five animals each treatment group. <i>(B)</i> Wild-type or CEAtg female mice were infected with <i>E</i>. <i>coli</i> AfaE-III or <i>E</i>. <i>coli</i> ΔAfaE-III for 24 h and the genital tracts processed as in (A). SEM pictures (at two different magnifications, as indicated by the scale bars) show the luminal surface of the upper vaginal and cervical regions. Whereas massive epithelial exfoliation is evident in infected wildtype mice and in CEAtg mice infected with <i>E</i>. <i>coli</i> ΔAfaE-III, a strongly reduced detachment of epithelial cells is observed in CEAtg mice infected with CEACAM-binding <i>E</i>. <i>coli</i> AfaE-III. Adherent bacteria can be observed at higher magnifications (arrows). Pictures are representative for five animals each treatment group. <i>(C)</i> Quantification of exfoliating epithelial cells from samples in (B). Bars represent mean ± S.D. of exfoliating cells from at least n = 26 areas (~0.075 mm²) derived from at least five animals each treatment group. Results were compared by Mann-Whitney U-test and highly significant differences (p<0.001) are indicated by ***.</p