Adsorption of mono- and multivalent cat- and anions on DNA molecules

Adsorption of monovalent and multivalent cat- and anions on a deoxyribose nucleic acid (DNA) molecule from a salt solution is investigated by computer simulation. The ions are modelled as charged hard spheres, the DNA molecule as a point charge pattern following the double-helical phosphate strands. The geometrical shape of the DNA molecules is modelled on different levels ranging from a simple cylindrical shape to structured models which include the major and minor grooves between the phosphate strands. The densities of the ions adsorbed on the phosphate strands, in the major and in the minor grooves are calculated. First, we find that the adsorption pattern on the DNA surface depends strongly on its geometrical shape: counterions adsorb preferentially along the phosphate strands for a cylindrical model shape, but in the minor groove for a geometrically structured model. Second, we find that an addition of monovalent salt ions results in an increase of the charge density in the minor groove while the total charge density of ions adsorbed in the major groove stays unchanged. The adsorbed ion densities are highly structured along the minor groove while they are almost smeared along the major groove. Furthermore, for a fixed amount of added salt, the major groove cationic charge is independent on the counterion valency. For increasing salt concentration the major groove is neutralized while the total charge adsorbed in the minor groove is constant. DNA overcharging is detected for multivalent salt. Simulations for a larger ion radii, which mimic the effect of the ion hydration, indicate an increased adsorbtion of cations in the major groove.Comment: 34 pages with 14 figure

Breech presentation at term and associated obstetric risks factors-a nationwide population based cohort study

Purpose The aim of this study was to estimate whether breech presentation at term was associated with known individual obstetric risk factors for adverse fetal outcome. Methods This was a retrospective, nationwide Finnish population-based cohort study. Obstetric risks in all breech and vertex singleton deliveries at term were compared between the years 2005 and 2014. A multivariable logistic regression model was used to determine significant risk factors. Results The breech presentation rate at term for singleton pregnancies was 2.4%. The stillbirth rate in term breech presentation was significantly higher compared to cephalic presentation (0.2 vs 0.1%). The odds ratios (95% CIs) for fetal growth restriction, oligohydramnios, gestational diabetes, a history of cesarean section and congenital fetal abnormalities were 1.19 CI (1.07-1.32), 1.42 CI (1.27-1.57), 1.06 CI (1.00-1.13), 2.13 (1.98-2.29) and 2.01 CI (1.92-2.11). Conclusions The study showed that breech presentation at term on its own was significantly associated with antenatal stillbirth and a number of individual obstetric risk factors for adverse perinatal outcomes. The risk factors included oligohydramnios, fetal growth restriction, gestational diabetes, history of caesarean section and congenital anomalies.Peer reviewe

Evaluating P-Band TomoSAR for Biomass Retrieval in Boreal Forest

P-band synthetic aperture radar (SAR) is sensitive to above-ground biomass (AGB) but retrieval accuracy has been shown to deteriorate in topographic areas. In boreal forest, the signal penetrates through the canopy to interact with the ground producing variations in backscatter depending on ground topography, forest structure, and soil moisture. Tomographic processing of multiple SAR images Tomographic SAR (TomoSAR) provides information about the vertical backscatter distribution. This article evaluates the use of P-band TomoSAR data to improve AGB retrievals from backscattered intensity by suppressing the backscattered signal from the ground. This approach can be used even when the tomographic resolution is insufficient to resolve the vertical backscatter profile. The analysis is based on P-band data from two campaigns: BioSAR-1 (2007) in Remingstorp, southern Sweden, and BioSAR-2 (2008) in Krycklan (KR), northern Sweden. BioSAR airborne data were also processed to correspond as closely as possible to future BIOMASS TomoSAR acquisitions, with BioSAR-2-based results shown. A power law AGB model using volumetric HV polarized backscatter performs best in KR, with training residual root mean-squared error (RMSE) of 30%-36% (27-33 t/ha) for airborne data and 38%-39% for simulated BIOMASS data. Airborne TomoSAR data suggest that both vertical and horizontal tomographic resolution are of importance and that it is possible to greatly reduce AGB retrieval bias when compared with airborne P-band SAR backscatter intensity-based retrievals. A lack of significant ground slopes in Remningstorp reduces the benefit of using TomoSAR data which performs similar to retrievals based solely on P-band SAR backscatter intensity