Tripanossomatídeos (protozoa: Kinetoplastida) Em Três Espécies De Cascudos Do Rio Mogi-guaçu, Pirassununga, São Paulo, Brasil

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Experimental infection of Aedes albopictus (Diptera : Culicidae) larvae with the xiphidiocercariae of a hematolechid

Aedes albopictus larvae were exposed, either individually or in groups, to different concentrations of xiphidiocercariae of Haematoloechus sp. for parasitological studies. It was observed the acute lethal effect and some aspects of the host-parasite relationship, such as delay or progress in the host life cycle, the number and location of the metacercariae in the host, adult host malformations and the amount of metacercariae required to cause death. A delay in the cycle and a high mortality rate was in general observed. Inside the larvae, the metacercariae were found predominantly in the thorax, abdominal segments and in the head, along with a reduced number in the anal lobe and cervix. It was shown that in addition to the quantity, of metacercariae present, their location in the larvae was also relevant in the determination of mortality and anomalies. Malformed adults developed from larvae containing from one to three metacercariae.97457357

Antigen, antibody and immune complex detection in serum samples from rats experimentally infected with Strongyloides venezuelensis

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)In order to establish an antigen, antibody and immune complex detection by enzyme-linked immunosorbent assay (ELISA) in serum samples, normal or immunocompromised Wistar rats experimentally infected with Strongyloides venezuelensis were used. The microtitre plates were coated with IgG anti-S. venezuelensis for antigen and immune complex detection and with alkaline parasite extract for antibody detection. Analysis revealed at least 12.5 mu g/mL of S. venezuelensis specific antigens in serum samples. Assay for antigen detection was not a good approach for evaluating infection in normal or immunocompromised rats. In normal rats IgG specific for S. venezuelensis was preferentially detected during the first 13 days post-infection (p.i.) and immune complex detection was significantly reduced in 21 p.i. day. On the other hand, in immunocompromised rats, IgG and immune complex were detected during the entire kinetic (5, 8, 13 and 21 p.i). These results suggest that immune complex screening seems to be an alternative for early strongyloidiasis diagnosis in immunocompromised individuals. (C) 2012 Elsevier Inc. All rights reserved.1303205208Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES

Hematological alterations caused by high intensity of L-3 larvae of Contracaecum sp Railliet & Henry, 1912 (Nematoda, Anisakidae) in the stomach of Hoplias malabaricus in lakes in Pirassununga, So Paulo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)The objective of the present study was to discover the hematological indices of Hoplias malabaricus infected by larvae of Contracaecum sp. A total of 105 fish were collected from two lakes located in the municipal district of Pirassununga, So Paulo, Brazil, of which 95.2 % were infested by L-3 larvae of Contracaecum sp., with an average intensity of 348.7 +/- 231.55 helminths/fish. A total of 76 fish were analyzed to establish hematological parameters. Following analysis, it was found that there was a significant difference between hematological parameters Hct, erythrocytes (Er), thrombocytopenia (p = 0.01), and Hb (p = 0.02) in lakes A and B, while there was no significant difference for leukocyte (p = 0.68), mean corpuscular volume, mean corpuscular hemoglobin (p = 0.06), and mean corpuscular hemoglobin concentration (p = 0.47). The intensity of infection correlated negatively with Er (r (s) = -0.42; p < 0.01) and positively for (r (s) = 0.48; p < 0.01). The high intensity of infection by Contracaecum sp. in H. malabaricus did not affect the health of the fish despite causing alterations in hematological variables.112827832789Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)CAPES [69/2010-UNICAMP