Phosphoinositide-dependent regulation of VAN3 ARF-GAP localization and activity essential for vascular tissue continuity in plants

ACAP-type ARF GTPase activating proteins (ARF-GAPs) regulate multiple cellular processes, including endocytosis, secretion, phagocytosis, cell adhesion and cell migration. However, the regulation of ACAP functions by other cellular proteins is poorly understood. We have reported previously that a plant ACAP, VAN3, plays a pivotal role in plant venation continuity. Here, we report on newly identified VAN3 regulators: the CVP2 (cotyledon vascular pattern 2) 5 PTase, which is considered to degrade IP3 and also to produce PtdIns(4) P from PtdIns(4,5) P-2; and a PH domain-containing protein, VAB (VAN3 binding protein). Combinational mutations of both CVP2 and its closest homologue CVL1 (CVP2 like 1) phenocopied the strong allele of van3 mutants, showing severe vascular continuity. The phenotype of double mutants between van3, cvp2 and vab suggested that VAN3, CVP2 and VAB function in vascular pattern formation in the same pathway. Localization analysis revealed that both CVP2 and VAB colocalize with VAN3 in the trans-Golgi network (TGN), supporting their functions in the same pathway. The subcellular localization of VAN3 was dependent on its PH domain, and mislocalization of VAN3 was induced in cvp2 or vab mutants. These results suggest that CVP2 and VAB cooperatively regulate the subcellular localization of VAN3 through the interaction between its PH domain and phosphoinositides and/or inositol phosphates. In addition, PtdIns(4) P, to which VAN3 binds preferentially, enhanced the ARF-GAP activity of VAN3, whereas IP3 inhibited it. These results suggest the existence of PtdIns(4) P and/or IP3-dependent subcellular targeting and regulation of VAN3 ACAP activity that governs plant vascular tissue continuity

Bayesian Approach to Find a Long-Term Trend in Erratic Polarization Variations Observed in Blazars

We developed a method to separate a long-term trend from observed temporal variations of polarization in blazars using a Bayesian approach. The temporal variation of the polarization vector is apparently erratic in most blazars, while several objects occasionally exhibited systematic variations, for example, an increase of the polarization degree associated with a flare of the total flux. We assume that the observed polarization vector is a superposition of distinct two components, a long-term trend and a short-term variation component responsible for short flares. Our Bayesian model estimates the long-term trend which satisfies the condition that the total flux correlates with the polarized flux of the short-term component. We demonstrate that assumed long-term polarization components are successfully separated by the Bayesian model for artificial data. We applied this method to photopolarimetric data of OJ 287, S5 0716+714, and S2 0109+224. Simple and systematic long-term trends were obtained in OJ 287 and S2 0109+224, while no such a trend was identified in S5 0716+714. We propose that the apparently erratic variations of polarization in OJ 287 and S2 0109+224 are due to the presence of the long-term polarization component. The behavior of polarization in S5 0716+714 during our observation period implies the presence of a number of polarization components having a quite short time-scale of variations.Comment: 12 pages, 7 figures, accepted for publication in PAS

Learning Large Causal Structures from Inverse Covariance Matrix via Matrix Decomposition

Learning causal structures from observational data is a fundamental yet highly complex problem when the number of variables is large. In this paper, we start from linear structural equation models (SEMs) and investigate ways of learning causal structures from the inverse covariance matrix. The proposed method, called $\mathcal{O}$-ICID (for {\it Independence-preserving} Decomposition from Oracle Inverse Covariance matrix), is based on continuous optimization of a type of matrix decomposition that preserves the nonzero patterns of the inverse covariance matrix. We show that $\mathcal{O}$-ICID provides an efficient way for identifying the true directed acyclic graph (DAG) under the knowledge of noise variances. With weaker prior information, the proposed method gives directed graph solutions that are useful for making more refined causal discovery. The proposed method enjoys a low complexity when the true DAG has bounded node degrees, as reflected by its time efficiency in experiments in comparison with state-of-the-art algorithms

Spectropolarimetric Study on Circumstellar Structure of Microquasar LS I +61deg 303

We present optical linear spectropolarimetry of the microquasar LS I +61$^{\circ}$ 303. The continuum emission is mildly polarized (up to 1.3 %) and shows almost no temporal change. We find a distinct change of polarization across the H$\alpha$ emission line, indicating the existence of polarization component intrinsic to the microquasar. We estimate the interstellar polarization (ISP) component from polarization of the H$\alpha$ line and derive the intrinsic polarization component. The wavelength dependence of the intrinsic component is well explained by Thomson scattering in equatorial disk of the Be-type mass donor. The position angle (PA) of the intrinsic polarization $\sim 25^{\circ}$ represents the rotational axis of the Be disk. This PA is nearly perpendicular to the PA of the radio jet found during quiescent phases. Assuming an orthogonal disk-jet geometry around the compact star, the rotational axis of the accretion disk is almost perpendicular to that of the Be disk. Moreover, according to the orbital parameters of the microquasar, the compact star is likely to get across the Be disk around their periastron passage. We discuss the peculiar circumstellar structure of this microquasar inferred from our observation and possible connection with its high-energy activities.Comment: 17pages, 7figures; accepted for Publications of the Astronomical Society of Japa

Photopolarimetric Monitoring of Blazars in the Optical and Near-Infrared Bands with the Kanata Telescope. I. Correlations between Flux, Color, and Polarization

We report on the correlation between the flux, color and polarization variations on time scales of days--months in blazars, and discuss their universal aspects. We performed monitoring of 42 blazars in the optical and near-infrared bands from 2008 to 2010 using TRISPEC attached to the "Kanata" 1.5-m telescope. We found that 28 blazars exhibited "bluer-when-brighter" trends in their whole or a part of time-series data sets. This corresponds to 88% of objects that were observed for >10 days. Thus, our observation unambiguously confirmed that the "bluer-when-brighter" trend is common in the emission from blazar jets. This trend was apparently generated by a variation component with a constant and relatively blue color and an underlying red component. Prominent short-term flares on time scales of days--weeks tended to exhibit a spectral hysteresis; their rising phases were bluer than their decay phases around the flare maxima. In contrast to the strong flux--color correlation, the correlation of the flux and polarization degree was relatively weak; only 10 objects showed significant positive correlations. Rotations of polarization were detected only in three objects: PKS 1510-089, 3C 454.3, and PKS 1749+096, and possibly in S5 0716+714. We also investigated the dependence of the degree of variability on the luminosity and the synchrotron peak frequency, \nu_peak. As a result, we found that lower luminosity and higher \nu_peak objects had smaller variations in their amplitudes both in the flux, color, and polarization degree. Our observation suggests the presence of several distinct emitting sources, which have different variation time-scales, colors, and polarizations. We propose that the energy injection by, for example, internal shocks in relativistic shells is a major factor for blazar variations on time scales of both days and months.Comment: 39 pages, accepted for publication in PAS

健常成人の対側，同側および両側耳への音刺激によるAEPの差異

Differences among AEPs evoked by left or right monaural, and binaural acoustic stimuli were studied with 40 healthy male subjects (21-33 y.o.). AEPs were evoked by click stimuli and derived from the five derivations and recorded averaging 100 responses, with 1024 msec of analysis time. The following results were obtained. 1) In all of five derivations, the contours of the group mean AEPs were roughly similar in both ipsilateral left and contralateral right monaural stimuli. These findings demonstrated that response characteristics in the cortex which contains receptive field of the two ears were well coincided with each other. 2) The latency of the N1 component was significantly shorter and the peak to peak amplitudes were significantly larger in both contralateral right monaural and binaural AEPs than in ipsilateral left monaural AEPs. These findings reconfirmed reports on both the hemispheric dominance of the contralateral ear and the stronger response of binaural stimuli than monaural stimuli. 3) The latency of the P2 component in contralateral right monaural AEPs was significantly longer than that in ipsilateral left monocular AEPs, and N 1-P 2, P 2-N 2 amplitudes were significantly larger. These findings supported the report that the sourse of P2 is the primary auditory area, and confirmed the hemispheric dominance of the contralateral ear. 4) The latencies of the long latency components in contralateral right monaural and binaural AEPs were significantly longer than those in ipsilateral left monaural AEPs, and their amplitudes were significantly larger. These findings suggested the existence of the complicated auditory imformation processing in the association cortex and long-recovery cycle system in the cortex